DNA Barcodes for Discriminating the Medicinal Plant Scutellaria baicalensis (Lamiaceae) and Its Adulterants

Guo, Xinfeng; Wang, Xiaoguo; Su, Wenhua; Zhang, Guangfei; Zhou, Rui

doi:10.1248/bpb.34.1198

Cited by 66 publications

(42 citation statements)

References 25 publications

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“…Although individual DNA barcodes were sufficient to discriminate each species, we strongly recommend using both barcode regions, as suggested for discriminating Scutellaria baicalensis from its adulterants in a previous report, because matK and rbcL did not exhibit unique marker nucleotides for A. erubescens and P. pedatisecta, respectively, and multilocus DNA barcodes are more effective for identification of plant species (Stoeckle, 2003;Guo et al, 2011;Purushothaman et al, 2014). In addition to the use of both matK and rbcL barcode regions, we also suggest using phylogenetic analyses to authenticate herbal medicines and their adulterants because phylogenetic clustering provides additional information that can be used for species discrimination on the basis of sequence divergence and co-relationships.…”

Section: Discussionmentioning

confidence: 89%

“…A desirable DNA barcode should contain sufficient sequence variation to permit species discrimination and show significant inter-species differences with minimal intra-species variation, together with convenient flanking regions for PCR amplification and sequencing (CBOL Plant Working Group, 2009;Guo et al, 2011). The CBOL proposed using a combination of matK and rbcL as a plant barcode due to the above-mentioned features (Saarela et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…These candidates are widely used as genetic markers to identify plant species and to analyze phylogenetic relationships (Kato et al, 1998;Kress and Erickson, 2007;Saarela et al, 2013). Chloroplast matK and rbcL DNA barcode regions have been used to identify species of diverse plant taxa, including herbal materials, and to clarify taxonomic origins (Cabrera et al, 2008;Sucher and Carles, 2008;Guo et al, 2011;Chao et al, 2014). In particular, DNA barcoding can provide objective tools that permit the authentication and identification of herbal medicines, especially for medicines that are often sold as dried slices or powders.…”

Section: Introductionmentioning

confidence: 99%

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Molecular identification of the traditional herbal medicines, Arisaematis Rhizoma and Pinelliae Tuber, and common adulterants via universal DNA barcode sequences

Moon

Kim

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Methods to identify Pinelliae Tuber and Arisaematis Rhizoma are required because of frequent reciprocal substitution between these two herbal medicines and the existence of several closely related plant materials. As a result of the morphological similarity of dried tubers, correct discrimination of authentic herbal medicines is difficult by conventional methods. Therefore, we analyzed DNA barcode sequences to identify each herbal medicine and the common adulterants at a species level. To verify the identity of these herbal medicines, we collected five authentic species (Pinellia ternata for Pinelliae Tuber, and Arisaema amurense, A. amurense var. serratum, A. erubescens, and A. heterophyllum for Arisaematis Rhizoma) and six common adulterant plant species. Maturase K (matK) and ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) genes were then amplified using universal primers. In comparative analyses of two DNA barcode sequences, we obtained 45 species-specific nucleotides sufficient to identify each species (except A. erubescens with matK) and 28 marker nucleotides for each species (except P. pedatisecta with rbcL). Sequence differences at corresponding positions of the two combined DNA barcodes provided genetic marker nucleotides that could be used to identify specimens of the correct species among the analyzed medicinal plants. Furthermore, we generated a phylogenetic tree showing nine distinct groups depending on the species. These results can be used to authenticate Pinelliae Tuber and Arisaematis Rhizoma from their adulterants and to identify each species. Thus, comparative analyses of plant DNA barcode sequences identified useful genetic markers for the authentication of Pinelliae Tuber and Arisaematis Rhizoma from several adulterant herbal materials.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular identification of the traditional herbal medicines, Arisaematis Rhizoma and Pinelliae Tuber, and common adulterants via universal DNA barcode sequences

Moon

Kim

et al. 2016

Genet. Mol. Res.

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“…Multilocus DNA barcodes have been proposed to be much effective and accurate in the identification of plant species (Group et al 2009). The rbcL has been proven to be an effective and accurate DNA barcode for the species identification of medicinal plants (Guo et al 2011). While, matK and rpoC1 would not be sufficient to solely identify the studied UAE plant species.…”

Section: Resultsmentioning

confidence: 99%

DNA barcoding of selected UAE medicinal plant species: a comparative assessment of herbarium and fresh samples

Enan

Palakkott

Ksiksi

2017

Physiol Mol Biol Plants

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It is commonly difficult to extract and amplify DNA from herbarium samples as they are old and preserved using different compounds. In addition, such samples are subjected to the accumulation of intrinsically produced plant substances over long periods (up to hundreds of years). DNA extraction from desert flora may pause added difficulties as many contain high levels of secondary metabolites. Herbarium samples from the Biology Department (UAE University) plant collection and fresh plant samples, collected from around Al-Ain (UAE), were used in this study. The three barcode loci for the coding genes matK, rbcL and rpoC1-were amplified. Our results showed that T. terresteris, H. robustum,T. pentandrus and Z. qatarense were amplified using all three primers for both fresh and herbaium samples. Both fresh and herbarium samples of C. comosum, however, were not amplified at all, using the three primers. Herbarium samples from A. javanica, C. imbricatum, T. aucherana and Z. simplex were not amplified with any of the three primers. For fresh samples 90, 90 and 80% of the samples were amplified using matK, rbcL and rpoC1, respectively. In short, fresh samples were significantly better amplified than those from herbarium sources, using the three primers. Both fresh and herbarium samples from one species (C. comosum), however, were not successfully amplified. It is also concluded that the rbcL regions showed real potentials to distinguish the UAE species under investigation into the appropriate family and genus.

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“…In recent years, several technologies such as morphology, chemical analysis (Xie et al, 2006), biological activity assay (Bai et al, 1997;Liu et al, 2006), and DNA molecular markers (Yip et al, 2007;Al-Qurainy et al, 2011;Guo et al, 2011) have been developed for authenticating raw herbal materials. With their characteristics of non-reliance on morphology, reproducibility, extreme reliability, and easy manipulation, DNA-based molecular tools are urgently needed in authenticating raw herbal materials.…”

Section: Resultsmentioning

confidence: 99%

Molecular authentication of multi-species honeysuckle tablets

Jiang

Yao²,

Chen³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Authenticating multi-species original raw materials in commercial formulations is difficult. Jin Yin Hua and Shan Yin Hua, both classified as raw honeysuckle materials in the Chinese Pharmacopoeia, are used in various medicines. Differentiating one variety from another is difficult based on chemical analysis. We developed molecular authentication of multi-species original honeysuckle in 3 brands of commercial tablets using allele-specific PCR. All 3 tablets contained both Jin Yin Hua and Shan Yin Hua. We also built a PCR-enzyme digestion method and enzymatic mutation detection in the PCR fragments of psbAtrnH and trnL-trnF, and the restriction endonucleases HinfI and NlaIV, respectively. The PCR-enzyme digestion method produced the same result as the allele-specific PCR. Sequence and phylogenetic analyses show that the tablets YXC and YQJ contained Lonicera japonica and L. macranthoides as original raw materials, and LYG contained L. japonica, L. hypoglauca, and L. macranthoides.

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DNA Barcodes for Discriminating the Medicinal Plant Scutellaria baicalensis (Lamiaceae) and Its Adulterants

Cited by 66 publications

References 25 publications

Molecular identification of the traditional herbal medicines, Arisaematis Rhizoma and Pinelliae Tuber, and common adulterants via universal DNA barcode sequences

Molecular identification of the traditional herbal medicines, Arisaematis Rhizoma and Pinelliae Tuber, and common adulterants via universal DNA barcode sequences

DNA barcoding of selected UAE medicinal plant species: a comparative assessment of herbarium and fresh samples

Molecular authentication of multi-species honeysuckle tablets

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