Molecular authentication of multi-species honeysuckle tablets

Jiang, Chao; Yao, Yuan; Chen, M.; Huang, Luqi

doi:10.4238/2013.october.22.2

Cited by 15 publications

(5 citation statements)

References 22 publications

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“…For certain species, an even higher frequency of substitution appears in turmeric powder (20%) 5 , Ginkgo biloba leaf (24.3%) 6 , black cohosh dietary supplements (25%) 7 , commercial teas (33%) 8 , and Rhodiola decoctions (60%) 9 . Similar to the situation for herbal dietary supplements, substitutions and counterfeits have also been documented in patented Chinese drugs 10 11 12 . Moreover, due to their heterogeneous origins and differences in preparation methods, the substitutes for patented Chinese drugs may be present at higher levels than indicated by current estimates.…”

mentioning

confidence: 81%

Fluorescence Visual Detection of Herbal Product Substitutions at Terminal Herbal Markets by CCP-based FRET technique

Jiang

Guang

Jin

et al. 2016

Sci Rep

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Inaccurate labeling of materials used in herbal products may compromise the therapeutic efficacy and may pose a threat to medicinal safety. In this paper, a rapid (within 3 h), sensitive and visual colorimetric method for identifying substitutions in terminal market products was developed using cationic conjugated polymer-based fluorescence resonance energy transfer (CCP-based FRET). Chinese medicinal materials with similar morphology and chemical composition were clearly distinguished by the single-nucleotide polymorphism (SNP) genotyping method. Assays using CCP-based FRET technology showed a high frequency of adulterants in Lu-Rong (52.83%) and Chuan-Bei-Mu (67.8%) decoction pieces, and patented Chinese drugs (71.4%, 5/7) containing Chuan-Bei-Mu ingredients were detected in the terminal herbal market. In comparison with DNA sequencing, this protocol simplifies procedures by eliminating the cumbersome workups and sophisticated instruments, and only a trace amount of DNA is required. The CCP-based method is particularly attractive because it can detect adulterants in admixture samples with high sensitivity. Therefore, the CCP-based detection system shows great potential for routine terminal market checks and drug safety controls.

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mentioning

confidence: 81%

Fluorescence Visual Detection of Herbal Product Substitutions at Terminal Herbal Markets by CCP-based FRET technique

Jiang

Guang

Jin

et al. 2016

Sci Rep

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“…Jin Yin Hua possesses biological and pharmaceutical properties such as antioxidant, antiangiogenic, antipyretic, antidiabetic, antibacterial, anti-inflammatory, antitumoral, antiviral, antiangiogenic, hepatoprotective, and antinociceptive activities. 37 Studies have shown its chemical components to have inhibitory actions on viruses including adenovirus, mice influenza virus, respiratory syncytial virus, herpes simplex virus 1 (HSV1), coxsackievirus B3, and parainfluenza. 38…”

Section: Lonicerae Japonicae ( J In Yín Hu A)mentioning

confidence: 99%

Properties of the Novel Chinese Herbal Medicine Formula Qu Du Qiang Fei I Hao Fang Warrant Further Research to Determine Its Clinical Efficacy in COVID-19 Treatment

Cruz

Trombley

Carrington

et al. 2021

Medical Acupuncture

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Introduction: COVID-19, the infectious disease induced by the virus severe acute respiratory syndromerelated coronavirus-2, has caused increasing global health concerns, and novel strategies to prevent or ameliorate the condition are needed. Traditional Chinese Medicine (TCM) herbal formulas have been used in the treatment of epidemics in China for over 2000 years. This study investigated the therapeutic effects of Qu Du

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“…Xu et al (2015) used SSR technology to construct SSR fingerprints in Excel format for six different types of L. japonica, providing the earliest data support for variety identification. In addition, there have been no studies on the identification of Lonicera varieties based on DNA molecular markers (Jiang et al, 2013). The SNP molecular marker technology used in this study refers to the DNA sequence polymorphism caused by the variation of a single base in the genomic DNA sequence (Gazendam et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Construction of SNP fingerprint and population genetic analysis of honeysuckle germplasm resources in China

Chang²,

Huang³

et al. 2023

Front. Plant Sci.

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IntroductionThe flower buds of Lonicera japonica Thunb. are widely used in Chinese medicine for their anti-inflammatory properties, and they have played an important role in the fight against SARS COVID-19 and other major epidemics. However, due to the lack of scientific and accurate variety identification methods and national unified standards, scattered and non-standardized management in flower bud production has led to mixed varieties that have caused significant difficulties in the cataloging and preservation of germplasm resources and the identification, promotion, and application of new L. japonica varieties.MethodsIn this study, we evaluated the population structure, genetic relationships, and genetic fingerprints of 39 germplasm resources of Lonicera in China using simplified genome sequencing technology.ResultsA total of 13,143,268 single nucleotide polymorphisms (SNPs) were identified. Thirty-nine samples of Lonicera were divided into four subgroups, and the population structure and genetic relationships among existing Lonicera germplasm resources were determined using principal component analysis, population structure analysis, and phylogenetic tree analysis. Through several stringent selection criteria, 15 additional streamlined, high-quality DNA fingerprints were filtered out of the validated 50 SNP loci and verified as being able to effectively identify the 39 Lonicera varieties.DiscussionTo our knowledge, this is the first comprehensive study measuring the diversity and population structure of a large collection of Lonicera varieties in China. These results have greatly broadened our understanding of the diversity, phylogeny, and population structure of Lonicera. The results may enhance the future analysis of genetic diversity, species identification, property rights disputes, and molecular breeding by providing a scientific basis and reference data for these efforts.

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Molecular authentication of multi-species honeysuckle tablets

Cited by 15 publications

References 22 publications

Fluorescence Visual Detection of Herbal Product Substitutions at Terminal Herbal Markets by CCP-based FRET technique

Fluorescence Visual Detection of Herbal Product Substitutions at Terminal Herbal Markets by CCP-based FRET technique

Properties of the Novel Chinese Herbal Medicine Formula Qu Du Qiang Fei I Hao Fang Warrant Further Research to Determine Its Clinical Efficacy in COVID-19 Treatment

Construction of SNP fingerprint and population genetic analysis of honeysuckle germplasm resources in China

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