DNA adduct formation by hormonal steroids in vitro

Seraj, Mohammed Jabed; Umemoto, Atsushi; Tanaka, Masaki; Kajikawa, Aiichiro; Hamada, Kazuhisa; Monden, Yasumasa

doi:10.1016/s0165-1218(96)90126-3

Cited by 19 publications

(6 citation statements)

References 24 publications

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“…However the hormonal replacement therapy has been reported to be associated with increased risk of cancer [19,20]. There are several reports on the genotoxic potential of estrogens and synthetic progestins in vivo and in vitro [21][22][23][24][25][26][27][28]. Most of the oral formulations have a definite proportion of estrogen and synthetic progesterone [29].…”

Section: Discussionmentioning

confidence: 99%

Effect of Oral Contraceptive Pills on the Blood Serum Enzymes and DNA Damage in Lymphocytes Among Users

et al. 2015

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The continuous use of synthetic hormones as contraceptive pill or hormonal replacement therapy among women is increasing day by day. The widespread use of different formulations as oral contraceptives by women throughout their reproductive cycle has given rise to a serious concern for studying the effects of oral contraceptives on enzymatic profile and DNA damage in peripheral blood lymphocytes among users. The present study was carried out on women taking oral contraceptives. The study was based on the questionnaire having the information of reproductive history, fasting, age, health, nature of menstrual cycle, bleeding and other disease. The profile of the blood serum enzymes i.e. alkaline phosphatase (ALP), gamma glutamyl transferase (GGT), lactate dehydrogenase (LDH), aminotransferases (SGOT and SGPT), serum proteins (albumin and globulin) and DNA damage in lymphocytes was studied among users and non-users. The results of the present study suggest that OCs not only effects enzymatic activity but also results in DNA damage that may vary with the duration of using oral contraceptives. A significant increase in LDH, GGT, SGPT, SGOT, globulin and decrease in ALP as well as albumin was found among users as compared to non-users. The observed DNA damage was more in users as compared to non-users. Hormonal contraceptives seem to exert DNA damage and also have significant effects on blood serum enzymes.

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Section: Discussionmentioning

confidence: 99%

Effect of Oral Contraceptive Pills on the Blood Serum Enzymes and DNA Damage in Lymphocytes Among Users

et al. 2015

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“…When testosterone was added at a final concentration of 2 mmol/l to DNA obtained from human surgical resections, rat liver, HepG2 cells, and calf thymus, did not form adducts with naked DNA. Furthermore, no adducts were observed in DNA isolated from HepG2 cells incubated with 10-100 mol/l testosterone for 24 h [39].…”

Section: Rapd Fingerprinting Patternmentioning

confidence: 91%

Androgenic profile and genotoxicity evaluation of testosterone propionate and novel synthesized heterocyclic steroids

Hana¹,

Khalil

Makawy

et al. 2008

The Journal of Steroid Biochemistry and Molecular Biology

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“…The IARC concluded that there is limited evidence for the carcinogenicity of progesterone in experimental animals and no evaluations of its carcinogenicity in humans can be made in the absence of epidemiological data (IARC,1979 , 1987) . Progesterone has shown no evidence of genotoxicity (IARC, 1987 ; Seraj et al, 1996) . In addition, progesterone did not induce any adverse effects on fertility and development in rats and rhesus monkeys (Wharton and Scott, 1964) .…”

Section: Potential Human Health Impacts Of Growth Hormones Used In Fomentioning

confidence: 99%

“…Increases of prostate gland weight and volume as well as amounts of serum testosterone, DHT, androstenedione, and estradiol were found by intramuscular injections of 200 mg of testosterone enanthate (equivalent to 8 mg/kg bw) for 28 weeks in adult male baboons (Karr et al, 1984) . Many studies on genotoxicity have shown that testosterone alone has no genotoxic potential (Han et al, 1995 ; Ho and Roy, 1994 ; Lasne et al, 1990 ; Seraj et al, 1996 ; Tsutsui et al, 1995) . Testosterone (10 mg) induced resorption of embryos in female SD rats when it was treated subcutaneously on day 10 of gestation (Sarkar et al, 1986) .…”

Section: Potential Human Health Impacts Of Growth Hormones Used In Fomentioning

confidence: 99%

Risk Assessment of Growth Hormones and Antimicrobial Residues in Meat

Jeong¹,

Kang²,

Lim³

et al. 2010

Toxicological Research

103

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Growth promoters including hormonal substances and antibiotics are used legally and illegally in food producing animals for the growth promotion of livestock animals. Hormonal substances still under debate in terms of their human health impacts are estradiol-17β, progesterone, testosterone, zeranol, trenbolone, and melengestrol acetate (MGA) . Many of the risk assessment results of natural steroid hormones have presented negligible impacts when they are used under good veterinary practices. For synthetic hormonelike substances, ADIs and MRLs have been established for food safety along with the approval of animal treatment. Small amounts of antibiotics added to feedstuff present growth promotion effects via the prevention of infectious diseases at doses lower than therapeutic dose. The induction of antimicrobial resistant bacteria and the disruption of normal human intestinal flora are major concerns in terms of human health impact. Regulatory guidance such as ADIs and MRLs fully reflect the impact on human gastrointestinal microflora. However, before deciding on any risk management options, risk assessments of antimicrobial resistance require large-scale evidence regarding the relationship between antimicrobial use in food-producing animals and the occurrence of antimicrobial resistance in human pathogens. In this article, the risk profiles of hormonal and antibacterial growth promoters are provided based on recent toxicity and human exposure information, and recommendations for risk management to prevent human health impacts by the use of growth promoters are also presented.

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DNA adduct formation by hormonal steroids in vitro

Cited by 19 publications

References 24 publications

Effect of Oral Contraceptive Pills on the Blood Serum Enzymes and DNA Damage in Lymphocytes Among Users

Effect of Oral Contraceptive Pills on the Blood Serum Enzymes and DNA Damage in Lymphocytes Among Users

Androgenic profile and genotoxicity evaluation of testosterone propionate and novel synthesized heterocyclic steroids

Risk Assessment of Growth Hormones and Antimicrobial Residues in Meat

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