DMBT1 is frequently downregulated in well-differentiated gastric carcinoma but more frequently upregulated across various gastric cancer types

Abstract

Well-differentiated gastric carcinomas are considered to represent a distinct entity emerging via specific molecular changes different from those found in other gastric carcinoma types. The gene deleted in malignant brain tumours 1 (DMBT1) at 10q25.3-q26.1 codes for a protein presumably involved in cell differentiation and protection and has been proposed as a candidate tumour suppressor for brain and epithelial cancer. One study reported a loss of DMBT1 expression in 12.5% (5/40) of gastric cancer samples. Here, we examined in more detail DMBT1 protein and mRNA expression in 78 primary gastric tumour samples and corresponding normal gastric mucosa. DMBT1 was expressed in all non-tumour gastric mucosa tissues. Eleven out of 71 (15%) gastric tumours were negative for the DMBT1 protein in immunohistochemical analyses. Lack of DMBT1 expression was significantly more frequently found in well-differentiated gastric tumours (6/18 well-differentiated tumours vs. 5/53 other subtypes; P=0.025). Quantitative RT-PCR revealed a downregulation of the DMBT1 mRNA for 8/21 (38%) cases, while the remaining 13 cases (62%) displayed a substantial upregulation. Our data suggest that a loss of DMBT1 expression may preferentially take place in well-differentiated gastric carcinoma. However, an upregulation of DMBT1 expression is more frequently found across all gastric cancer types.

Gastric De Novo Muc13 Expression and Spasmolytic Polypeptide-Expressing Metaplasia during Helicobacter heilmannii Infection

“…7A). The mRNA expression level of Dmbt1, which has a function in epithelial cell differentiation and gastric mucosal protection (24), was highly increased between 20 and 52 weeks postinfection in the fundus of H. heilmannii-and H. pylori-infected mice (Fig. 7B).…”

Deleted in malignant brain tumor 1 is a novel prognostic marker in colorectal cancer

“…Infrequent mutation of DMBT1 has been reported in astrocytic tumors (19). In addition, upregulation of DMBT1 has been reported in specific glioblastomas (20) and carcinomas of the stomach (21) and lung (22). These diverse observations imply complex roles of DMBT1 in carcinogenesis.…”

The mouth: a gateway or a trap for HIV?

“…Prakobphol et al [89] using a proteomic approach demonstrated that SAG was identical to the previously identified and cloned lung Scavenger Receptor Cysteine-Rich (SRCR) glycoprotein gp340, which is a product of the DMBT1 gene. There is an extensive literature relating to DMBT-1 functioning as a tumor suppressor in a variety of epithelial derived tumors [90–92]. …”