“…Therefore, we focused on E3 ubiquitin ligases reported to target ΔNp63: Fbw7 (Galli et al, 2010; Restelli et al, 2014), Itch (Rossi et al, 2006a; Rossi et al, 2006b), HDM2 (Galli et al, 2010), Pirh2 (Jung et al, 2013; Yan et al, 2013), and WWP1 (Li et al, 2008). Colo16 cells were transfected with siRNAs against each of these factors individually and treated with the HDACi, JNJ-26481585, or the vehicle alone, DMSO.…”