DLP printed hDPSC-loaded GelMA microsphere regenerates dental pulp and repairs spinal cord

Qian, Ying; Gong, Jiaxing; Lu, Kaining; Hong, Yi; Zhu, Z.H.; Zhang, Jingyu; Zou, Yiwei; Zhou, Feifei; Zhang, Chaoying; Zhou, Siyi; Gu, Tianyi; Sun, Miao; Wang, Shaolong; He, Jianxiang; Yang, Li; Lin, Junxin; Yuan, Yuan; Ouyang, Hongwei; Yu, Mengfei; Wang, Huiming

doi:10.1016/j.biomaterials.2023.122137

Cited by 24 publications

(21 citation statements)

References 58 publications

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“…The findings revealed the formation of vascularized 3c). 53 The DLP-printed hDPSCs microspheres exhibited excellent biocompatibility, dryness, and multidirectional differentiation potential. The study revealed that hDPSCs-loaded microspheres could regenerate neural tissue at both tissue and functional levels, resulting in vascularization and neurogenesis of dental pulp.…”

Section: Gelatin Methacrylate (Gelma)mentioning

confidence: 94%

“…Qian et al. employed DLP technology to fabricate GelMA microspheres encapsulating human dental pulp stem cells (hDPSCs), investigating cellular heterogeneity after cell expansion through single-cell RNA sequencing, and conducting a series of characterizations on the microspheres (Figure c) . The DLP-printed hDPSCs microspheres exhibited excellent biocompatibility, dryness, and multidirectional differentiation potential.…”

Section: Functionalization Of Gelatinmentioning

confidence: 99%

Section: Functionalization Of Gelatinmentioning

confidence: 99%

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Chemical and Structural Engineering of Gelatin-Based Delivery Systems for Therapeutic Applications: A Review

Jia,

Fan,

Chen

et al. 2024

Biomacromolecules

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As a biodegradable and biocompatible protein derived from collagen, gelatin has been extensively exploited as a fundamental component of biological scaffolds and drug delivery systems for precise medicine. The easily engineered gelatin holds great promise in formulating various delivery systems to protect and enhance the efficacy of drugs for improving the safety and effectiveness of numerous pharmaceuticals. The remarkable biocompatibility and adjustable mechanical properties of gelatin permit the construction of active 3D scaffolds to accelerate the regeneration of injured tissues and organs. In this Review, we delve into diverse strategies for fabricating and functionalizing gelatin-based structures, which are applicable to gene and drug delivery as well as tissue engineering. We emphasized the advantages of various gelatin derivatives, including methacryloyl gelatin, polyethylene glycolmodified gelatin, thiolated gelatin, and alendronate-modified gelatin. These derivatives exhibit excellent physicochemical and biological properties, allowing the fabrication of tailor-made structures for biomedical applications. Additionally, we explored the latest developments in the modulation of their physicochemical properties by combining additive materials and manufacturing platforms, outlining the design of multifunctional gelatin-based micro-, nano-, and macrostructures. While discussing the current limitations, we also addressed the challenges that need to be overcome for clinical translation, including high manufacturing costs, limited application scenarios, and potential immunogenicity. This Review provides insight into how the structural and chemical engineering of gelatin can be leveraged to pave the way for significant advancements in biomedical applications and the improvement of patient outcomes.

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Section: Gelatin Methacrylate (Gelma)mentioning

confidence: 94%

Section: Functionalization Of Gelatinmentioning

confidence: 99%

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Chemical and Structural Engineering of Gelatin-Based Delivery Systems for Therapeutic Applications: A Review

Jia,

Fan,

Chen

et al. 2024

Biomacromolecules

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“…This endows photocuring crosslinking and thermal response capabilities, leading to exceptional printability [142]. Photocuring can be utilized for crosslinking while printing layerby-layer from bottom to top, allowing a precise 3D spinal cord structure to be generated while ensuring the stability of each layer so the scaffold structure does not collapse [52,143]. For example, a 3D GelMA hydrogel was found to sustain the survival and differentiation of photo-encapsulated iPSC-derived NSCs [33].…”

Section: −Hydrophobicity −Slow Degradation Rate −Inhibits Cell Adhesionmentioning

confidence: 99%

“…(I) Cell survival after 3D bioprinting. Reprinted from[52], Copyright (2023), with permission from Elsevier. (J) Immunofluorescence images of encapsulated cells in GelMA with 3D printed hydrogels, which were treated without or with ES for 2 d. Cells were stained with neurofilament (green), Tuj1 (red), and cell nuclei (blue).…”

mentioning

confidence: 99%

3D bioprinting approaches for spinal cord injury repair

Jiu,

Liu,

et al. 2024

Biofabrication

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Regenerative healing of spinal cord injury poses an ongoing medical challenge by causing persistent neurological impairment and a significant socioeconomic burden. The complexity of spinal cord tissue presents hurdles to successful regeneration following injury, due to the difficulty of forming a biomimetic structure that faithfully replicates native tissue using conventional tissue engineering scaffolds. 3D bioprinting is a rapidly evolving technology with unmatched potential to create 3D biological tissues with complicated and hierarchical structure and composition. With the addition of biological additives such as cells and biomolecules, 3D bioprinting can fabricate preclinical implants, tissue or organ-like constructs, and in vitro models through precise control over the deposition of biomaterials and other building blocks. This review highlights the characteristics and advantages of 3D bioprinting for scaffold fabrication to enable spinal cord injury repair, including bottom-up manufacturing, mechanical customization, and spatial heterogeneity. This review also critically discusses the impact of various fabrication parameters on the efficacy of spinal cord repair using 3D bioprinted scaffolds, including the choice of printing method, scaffold shape, biomaterials, and biological supplements such as cells and growth factors. High-quality preclinical studies are required to accelerate the translation of 3D bioprinting into clinical practice for spinal cord repair. Meanwhile, other technological advances will continue to improve the regenerative capability of bioprinted scaffolds, such as the incorporation of nanoscale biological particles and the development of 4D printing.

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Injectable Nano‐Micro Composites with Anti‐bacterial and Osteogenic Capabilities for Minimally Invasive Treatment of Osteomyelitis

Lu,

Zhao,

Wang

et al. 2024

Advanced Science

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The effective management of osteomyelitis remains extremely challenging due to the difficulty associated with treating bone defects, the high probability of recurrence, the requirement of secondary surgery or multiple surgeries, and the difficulty in eradicating infections caused by methicillin‐resistant Staphylococcus aureus (MRSA). Hence, smart biodegradable biomaterials that provide effective and precise local anti‐infection effects and can promote the repair of bone defects are actively being developed. Here, a novel nano‐micro composite is fabricated by combining calcium phosphate (CaP) nanosheets with drug‐loaded GelMA microspheres via microfluidic technology. The microspheres are covalently linked with vancomycin (Van) through an oligonucleotide (oligo) linker using an EDC/NHS carboxyl activator. Accordingly, a smart nano‐micro composite called “CaP@MS‐Oligo‐Van” is synthesized. The porous CaP@MS‐Oligo‐Van composites can target and capture bacteria. They can also release Van in response to the presence of bacterial micrococcal nuclease and Ca2+, exerting additional antibacterial effects and inhibiting the inflammatory response. Finally, the released CaP nanosheets can promote bone tissue repair. Overall, the findings show that a rapid, targeted drug release system based on CaP@MS‐Oligo‐Van can effectively target bone tissue infections. Hence, this agent holds potential in the clinical treatment of osteomyelitis caused by MRSA.

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DLP printed hDPSC-loaded GelMA microsphere regenerates dental pulp and repairs spinal cord

Cited by 24 publications

References 58 publications

Chemical and Structural Engineering of Gelatin-Based Delivery Systems for Therapeutic Applications: A Review

Chemical and Structural Engineering of Gelatin-Based Delivery Systems for Therapeutic Applications: A Review

3D bioprinting approaches for spinal cord injury repair

Injectable Nano‐Micro Composites with Anti‐bacterial and Osteogenic Capabilities for Minimally Invasive Treatment of Osteomyelitis

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