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Gouveia, Ester Ribeiro; Neto, Álvaro de Baptista; Badino, Alberto Colli; Hokka, Carlos O.

doi:10.1023/a:1010356231673

Cited by 20 publications

(10 citation statements)

References 10 publications

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“…The productivity of microbial metabolites is, in general, closely related to the fermentation process (6,12,18). In order to reduce the costs of a bioprocess, it is necessary to develop strains with increased productivity, use inexpensive raw materials, improve filtration properties, perform the process under favorable conditions (30), thus requiring its optimization.…”

Section: Streptomyces Jumonjinensis Streptomyces Katsurahamanusmentioning

confidence: 99%

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Optimization of clavulanic acid production by Streptomyces daufpe 3060 by response surface methodology

Marques

Cunha

Araújo

et al. 2011

Braz. J. Microbiol.

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Clavulanic acid is a β-lactam antibiotic which has a potent β-lactamase inhibiting activity. In order to optimize its production by the new isolate Streptomyces DAUFPE 3060, the influence of two independent variables, temperature and soybean flour concentration, on clavulanic acid and biomass concentrations was investigated in 250 mL-Erlenmeyers according to a 2² central composite design. To this purpose, temperature and soybean flour (SF) concentration were varied in the ranges 26-34°C and 10-50 g/L, respectively, and the results evaluated utilizing the Response Surface Methodology. The experimental maximum production of clavulanic acid (629 mg/L) was obtained at 32°C and 40 g/L SF after 48 h, while the maximum biomass concentration (3.9 g/L) at 30°C and 50 g/L soybean flour, respectively. These values are satisfactorily close to those (640 mg/L and 3.75 g/L, respectively) predicted by the model, thereby demonstrating the validity of the mathematical approach adopted in this study

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Section: Streptomyces Jumonjinensis Streptomyces Katsurahamanusmentioning

confidence: 99%

“…These results are of particular concern, when compared supplemented with amino acids (31), and in a glycerol-based medium with protein extract of soybean (1178 mg/L) (12).…”

Section: Relationship Between Ca Production and Biomass Growthmentioning

confidence: 99%

Optimization of clavulanic acid production by Streptomyces daufpe 3060 by response surface methodology

Marques

Cunha

Araújo

et al. 2011

Braz. J. Microbiol.

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“…The types of nutrients, their concentrations, and the operating conditions have different effects on the accumulation of metabolites, which is controlled by intracellular effectors [7][8][9]. Selection of the most suited culture medium composition is of primary importance to increase the productivity of any bioprocess.…”

Section: Introductionmentioning

confidence: 99%

Screening of Variables Influencing the Clavulanic Acid Production by Streptomyces DAUFPE 3060 Strain

Viana

Carneiro-Cunha

Araújo

et al. 2009

Appl Biochem Biotechnol

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Clavulanic acid (CA) is a beta-lactam antibiotic, which has a potent beta-lactamase inhibiting activity. The influence of five variables, namely pH (6.0, 6.4, and 6.8), temperature (28 degrees C, 30 degrees C, and 32 degrees C), agitation intensity (150, 200, and 250 rpm), glycerol concentration (5.0, 7.5, and 10 g/L) and soybean flour concentration (5.0, 12.5, and 20 g/L), on CA production by a new isolate of Streptomyces (DAUFPE 3060) was investigated in 250-mL Erlenmeyer flasks using a fractional factorial design. Temperature and soybean flour concentration were shown to be the two variables that exerted the most important effects on the production of CA at 95% confidence level. The highest CA concentration (494 mg/L) was obtained after 48 h at 150 rpm, 32 degrees C, pH 6.0, 5.0 g/L glycerol, and 20 g/L soybean flour concentrations. Under these conditions, the yields of biomass and product on consumed substrate were 0.26 g(X)/g(S) and 64.3 mg(P)/g(S), respectively. Fermentations performed in 3.0-L bench-scale fermenter allowed increasing the CA production by about 60%.

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“…The number of test groups is relatively small, which saves human and material resources. This method has been applied to optimize a variety of biological processes (DeMeo et al 1985;Rama et al 1999;Ester et al 2001). In this study, a Box-Behnken design was used to arrange the testing levels (Table 2) (Box and Behnken 1960).…”

Section: Methodsmentioning

confidence: 99%

Investigation of Shear Strength of Engineered Wood Flooring Bonded with PUR by Response Surface Methodology

et al. 2017

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The effects of process parameters (adhesive spreading rate, pressing time, and applied pressure) on the response parameter (shear strength) of engineered wood flooring bonded with polyurethane (PUR) were studied by response surface methodology. A mathematical model was established to provide a satisfying prediction for the experimental results observed. The model indicated a high degree of fitting. The results also showed that the major factors were adhesive spreading rate and pressing time. The optimized parameters for highest shear strength of engineered wood flooring were 98 g/m 2 adhesive spreading rate with pressing time of 24 s and applied pressure of 1.2 MPa, respectively, within certain ranges.

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Untitled

Cited by 20 publications

References 10 publications

Optimization of clavulanic acid production by Streptomyces daufpe 3060 by response surface methodology

Optimization of clavulanic acid production by Streptomyces daufpe 3060 by response surface methodology

Screening of Variables Influencing the Clavulanic Acid Production by Streptomyces DAUFPE 3060 Strain

Investigation of Shear Strength of Engineered Wood Flooring Bonded with PUR by Response Surface Methodology

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