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Eymar, E.; Alegre, Julio; Toribio, M.; López‐Vela, Dolores

doi:10.1023/a:1006471519609

Cited by 27 publications

(6 citation statements)

References 16 publications

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“…Paek and Murthy (1977) have reported that activated charcoal prevents light entry to the environment and increases rooting. Moreover, addition of activated charcoal increases and keeps constant the level of pH of the culture medium and encourages growth by increasing nitrogen uptake (Eymar et al, 2000). The promotional effects of activated charcoal on morphogenesis may be also mainly due to its irreversible adsorption of inhibitory compounds in the culture medium and substantially decreasing the toxic metabolites, phenolic exudation, and brown exudate accumulation (Thomas, 2008).…”

Section: Seed Germination and Shoot Formationmentioning

confidence: 99%

In vitro seed germination, plantlet growth, tuberization, and syntheticseed production of Serapias vomeracea (Burm.f.) Briq.

Bektaş¹,

Sökmen²

2016

Turk J Bot

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Orchids are considered recalcitrant plants in in vitro propagation. Due to the lack of appropriate micropropagation techniques for mass production and damage to their ecological distribution posed by local gatherers, these species are threatened with extinction, including Serapias vomeracea (Burm.f.) Briq. In this research, we put forward a complete micropropagation method covering in vitro micropropagation, synthetic seed formation, germination in soil, and acclimatization to ambient conditions. To the best of our knowledge this is the first report of successful synthetic seed formation and germination of S. vomeracea. Initially, seeds were germinated in different culture media and also media supplemented with different concentrations of plant growth regulators. Effects of plant growth regulators on tuber formation, glucomannan contents, and different growth parameters were evaluated throughout the study. The best germination rate (84.03%) was achieved on Orchimax including activated charcoal medium and supplemented with 2.0 mg/L 6-benzyladenine. The longest shoot elongation amongst plantlets was observed on the same medium supplemented with 0.25 mg/L thidiazuron, whereas 2.0 mg/L indole-3-butyric acid favored leaf formation. Higher indole-3-butyric acid concentrations were found to be more effective in the formation and elongation of roots. Orchimax medium supplemented with zeatin (2.0 mg/L) was superior to the others in terms of tuber formation and glucomannan content therein. Adaptation of seedlings to soil conditions and germination abilities of synthetic seeds were also studied and seedlings were successfully acclimatized and adapted to soil conditions.

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Section: Seed Germination and Shoot Formationmentioning

confidence: 99%

In vitro seed germination, plantlet growth, tuberization, and syntheticseed production of Serapias vomeracea (Burm.f.) Briq.

Bektaş¹,

Sökmen²

2016

Turk J Bot

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“…It may also stimulate rooting by absorbing the toxins and excluding light from the medium (Paek and Murthy 1977;Yam et al 1989). Eymar et al (2000) observed that the addition of activated charcoal increased and maintained pH levels during culture, increased the nitrogen uptake and improved growth and visual aspects of the explants and reduced the inhibitory effect of exogenous cytokinin on root growth. However, activated carbon is likely to interfere with other additives as well.…”

Section: Genus: Cattleyamentioning

confidence: 96%

In Vitro Propagation and Germplasm Conservation of Wild Orchids from South America

Dolce

Medina

Terada

et al. 2020

Orchid Biology: Recent Trends &Amp; Challenges

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Orchids are an important part of plant biodiversity on the planet due to their high variability among species and their habitats. South America presents more than thirty percent of all known orchid species, being Colombia, Ecuador, Brazil, Peru, and Bolivia between the richest countries in the world in terms of orchid biodiversity. Nevertheless, concerning to the orchid conservation status, in Colombia precisely orchids occupy the unlucky first place as the plant family with the highest number of threatened species. Similar situation is happening in the rest of the South American countries. The two main threats to orchid survival are both anthropogenic: the first one is deforestation, and the second largest threat to orchids is collection from the wild. One desirable action to safeguard these endangered species is to develop procedures that make possible their massive propagation, which should provide material for the eco-rehabilitation of specimens into their natural habitats, the exchange with other entities, the supply to orchid merchants for avoid extractions of nature, and the availability of material for future research. Likewise, the development of systems that allow the ex situ conservation of orchid germplasm is imperative. This chapter reviews the progresses of different in vitro approaches for orchid propagation and germplasm conservation, safeguarding the genetic biodiversity of these species. Several study cases are presented and described to exemplify the protocols developed in the Botanical Institute of Northeast (UNNE-CONICET) for propagating and long-term storing the germplasm of wild orchids from Argentina (Cattleya lundii, Cohniella cepula, Cohniella jonesiana, Gomesa bifolia, Aa achalensis, Cyrtopodium brandonianum, Cyrtopodium hatschbachii, Habenaria bractescens). Moreover, it has been attempted to put together most of the available literature on in vitro propagation and germplasm conservation for South American orchids using different explants and procedures. There are researches of good scientific quality that even cover critical insights into the physiology and factors affecting growth and development as well as storage of several orchid materials. Anyway, studies are still necessary to cover a mayor number of South American species as well as the use of selected material (clonal) for both propagation and conservation approaches.

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“…Un ejemplo de estas sustancias es el 5hidroximetil-furfural, el cual inhibe el crecimiento vegetal (Thomas, 2008). Otras de las sustancias absorbidas por el carbón activado son liberadas por el tejido vegetal inoculado en el medio, y otras son ingredientes del medio de cultivo (Eymar et al, 2000). Pero el carbón activado tiende adsorber primero las sustancias moderadamente polares, y adsorbe mejor los compuestos aromáticos que los compuestos olefínicos insaturados.…”

Section: Introductionunclassified

“…Por otra parte, el carbón activado altera el pH del medio, lo cual es sumamente importante ya que el pH del medio afecta el crecimiento del tejido vegetal y los procesos morfogénicos, debido a que altera la disponibilidad y el consumo de los nutrientes (Eymar et al, 2000).…”

Section: Introductionunclassified

“…Lo anterior ocurre por el efecto que tiene el carbón activado sobre el pH del medio. Ya se han hecho pruebas con medio para el cultivo in vitro de tejidos vegetales, ajustando el pH del medio a 5.7, previo al autoclavado, y se ha observado que el medio de cultivo libre de carbón activado puede sufrir una disminución de su pH después de la esterilización, adquiriendo un pH de 4.67, mientras que un medio con la misma composición pero suplementado con 5 g/L de carbón activado puede sufrir una elevación de su pH a 6.08 (Eymar et al, 2000).…”

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Disinfection of adult pecan leaflets Carya illinoinensis (Wangenh.) K. Koch. (Juglandaceae), and in vitro callogenesis induction

et al. 2015

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Untitled

Cited by 27 publications

References 16 publications

In vitro seed germination, plantlet growth, tuberization, and syntheticseed production of Serapias vomeracea (Burm.f.) Briq.

In vitro seed germination, plantlet growth, tuberization, and syntheticseed production of Serapias vomeracea (Burm.f.) Briq.

In Vitro Propagation and Germplasm Conservation of Wild Orchids from South America

Disinfection of adult pecan leaflets Carya illinoinensis (Wangenh.) K. Koch. (Juglandaceae), and in vitro callogenesis induction

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