DJ-1–Mediated Protective Effect of Protocatechuic Aldehyde Against Oxidative Stress in SH-SY5Y Cells

Gao, Jianwei; Yamane, Toshimi; Maita, Hiroshi; Ishikawa, Sayaka; Iguchi‐Ariga, Sanae M.M.; Pu, Xiaoping; Ariga, Hiroyoshi

doi:10.1254/jphs.10271fp

Cited by 27 publications

(25 citation statements)

References 39 publications

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“…In several animal models, including cerebral ischemia model (Guo et al, 2016) and experimental model of sepsis (Xu et al, 2012). PA has shown pharmacological effects on inflammation and oxidative stress, which are highly correlated with pathogenesis of cisplatin nephropathy (Gao et al, 2011; Wei et al, 2013). For example, PA alleviated cerebral ischemia-reperfusion-induced oxidative injury via activating protein kinase C𝜀/Nrf2/HO-1 pathway (Guo et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Protocatechuic Aldehyde Attenuates Cisplatin-Induced Acute Kidney Injury by Suppressing Nox-Mediated Oxidative Stress and Renal Inflammation

et al. 2016

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Cisplatin is a classic chemotherapeutic agent widely used to treat different types of cancers including ovarian, head and neck, testicular and uterine cervical carcinomas. However, cisplatin induces acute kidney injury by directly triggering an excessive inflammatory response, oxidative stress, and programmed cell death of renal tubular epithelial cells, all of which lead to high mortality rates in patients. In this study, we examined the protective effect of protocatechuic aldehyde (PA) in vitro in cisplatin-treated tubular epithelial cells and in vivo in cisplatin nephropathy. PA is a monomer of Traditional Chinese Medicine isolated from the root of S. miltiorrhiza (Lamiaceae). Results show that PA prevented cisplatin-induced decline of renal function and histological damage, which was confirmed by attenuation of KIM1 in both mRNA and protein levels. Moreover, PA reduced renal inflammation by suppressing oxidative stress and programmed cell death in response to cisplatin, which was further evidenced by in vitro data. Of note, PA suppressed NAPDH oxidases, including Nox2 and Nox4, in a dosage-dependent manner. Moreover, silencing Nox4, but not Nox2, removed the inhibitory effect of PA on cisplatin-induced renal injury, indicating that Nox4 may play a pivotal role in mediating the protective effect of PA in cisplatin-induced acute kidney injury. Collectively, our data indicate that PA blocks cisplatin-induced acute kidney injury by suppressing Nox-mediated oxidative stress and renal inflammation without compromising anti-tumor activity of cisplatin. These findings suggest that PA and its derivatives may serve as potential protective agents for cancer patients receiving cisplatin treatment.

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Section: Discussionmentioning

confidence: 99%

Protocatechuic Aldehyde Attenuates Cisplatin-Induced Acute Kidney Injury by Suppressing Nox-Mediated Oxidative Stress and Renal Inflammation

et al. 2016

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“…For example, 3,4-DHBA inhibits the abnormal migration and proliferation of vascular smooth muscle cells induced by the ROS generated by platelet-derived growth factor [19]. In addition, it has been reported that 3,4-DHBA has anti-inflammatory activities [3], neuroprotective effects against oxidative stress [10] and anti-cancer activities [13]. …”

Section: Discussionmentioning

confidence: 99%

3,4-Dihydroxybenzaldehyde Derived from <i>Prunus mume</i> Seed Inhibits Oxidative Stress and Enhances Estradiol Secretion in Human Ovarian Granulosa Tumor Cells

Kono

Nomura

Okuno

et al. 2014

Acta Histochem. Cytochem.

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Granulosa cells form ovarian follicles and play important roles in the growth and maturation of oocytes. The protection of granulosa cells from cellular injury caused by oxidative stress is an effective therapy for female infertility. We here investigated an effective bioactive compound derived from Prunus mume seed extract that protects granulosa cells from hydrogen peroxide (H2O2)-induced apoptosis. We detected the bioactive compound, 3,4-dihydroxybenzaldehyde (3,4-DHBA), via bioactivity-guided isolation and found that it inhibited the H2O2-induced apoptosis of granulosa cells. We also showed that 3,4-DHBA promoted estradiol secretion in granulosa cells and enhanced the mRNA expression levels of steroidogenic factor 1, a promoter of key steroidogenic enzymes. These results suggest that P. mume seed extract may have clinical potential for the prevention and treatment of female infertility.

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“…The oxidative state of Cys106 of PARK7 was identified by LTQ Orbitrap mass spectrometry analysis as described previously (Gao et al 2011) with some modifications. The spermatozoa proteins were concentrated by centrifugation in ultrafiltration tubes (Millipore) at about 8 mg/ml.…”

Section: Immunoprecipitation and Mass Spectrometry Analysismentioning

confidence: 99%

PARK7 protein translocating into spermatozoa mitochondria in Chinese asthenozoospermia

Sun¹,

Zhang²,

Zhao³

et al. 2014

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PARK7 (DJ1) is a multifunctional oxidative stress response protein that protects cells against reactive oxygen species (ROS) and mitochondrial damage. PARK7 defects are known to cause various physiological dysfunctions, including infertility. Asthenozoospermia (AS), i.e. low-motile spermatozoa in the ejaculate, is a common cause of human male infertility. In this study, we found that downregulation of PARK7 resulted in increased levels of lipid peroxide and ROS, decreased mitochondrial membrane potential, and reduced mitochondrial complex I enzyme activity in the spermatozoa from AS patients. Furthermore, it was observed that PARK7 was translocated into the mitochondria of damaged spermatozoa in AS. Finally, we examined the oxidative state of PARK7 and the results demonstrated the enhancement of oxidation, expressed by increased sulfonic acid residues, the highest form of oxidation, as the sperm motility decreased. Taken together, these results revealed that PARK7 deficiency may increase the oxidative stress damage to spermatozoa. Our present findings open new avenues of therapeutic intervention targeting PARK7 for the treatment of AS.

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DJ-1–Mediated Protective Effect of Protocatechuic Aldehyde Against Oxidative Stress in SH-SY5Y Cells

Cited by 27 publications

References 39 publications

Protocatechuic Aldehyde Attenuates Cisplatin-Induced Acute Kidney Injury by Suppressing Nox-Mediated Oxidative Stress and Renal Inflammation

Protocatechuic Aldehyde Attenuates Cisplatin-Induced Acute Kidney Injury by Suppressing Nox-Mediated Oxidative Stress and Renal Inflammation

3,4-Dihydroxybenzaldehyde Derived from <i>Prunus mume</i> Seed Inhibits Oxidative Stress and Enhances Estradiol Secretion in Human Ovarian Granulosa Tumor Cells

PARK7 protein translocating into spermatozoa mitochondria in Chinese asthenozoospermia

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