Diversity of trypsins in the Mediterranean corn borer Sesamia nonagrioides (Lepidoptera: Noctuidae), revealed by nucleic acid sequences and enzyme purification

Stage-specific gut proteinases of the cotton stainer bug Dysdercus peruvianus: Role in the release of entomotoxic peptides from Canavalia ensiformis urease

“…The data on azocasein hydrolysis suggested that adults have trypsin-like enzymes, while PMSF-sensitive enzymes were not found in nymphs. Dıaz-Mendoza et al (2005) reported that E-64 nonspecifically inhibited trypsin-1 enzymes from insects. The more pronounced effect of E-64 on AH activity upon the C-terminal substrate could thus represent the inhibition of cysteine proteinases plus nonspecific inhibition of trypsin-like enzymes.…”

Cloning and characterization of cDNAS encoding carboxypeptidase-like proteins from the gut of Hessian fly larvae [Mayetiola destructor (Say)]☆

“…Numerous digestive proteases and amylases have been characterized from many agriculturally important insects (Bown et al, 1998;Gaines et al, 1999;Cristofoletti et al, 2001;Coffeen and Wolpert, 2004;Gruden et al, 2004;Diaz-Mendoza et al, 2005;Zhu et al, 2005). Subsequently, various proteinaceous inhibitors have been identified against these enzymes from different sources (Farmer et al, 1992;Zhao et al, 1996;Koiwa et al, 2000;Moura and Ryan, 2001).…”

Structural and functional characterization of solution, gel, and aggregated forms of trypsin in organic solvent-assisted and pH-induced phase changes

“…Other conditions may also cause aggregation of the protein molecules which have amorphous physical appereance. As opposed to gelation, protein aggregation/precipitation is considered to be a phase separation resulting in formation of a solid phase in a solution [2][3]. The aim of this research is to compare the solution, gel and aggregate forms of trypsin obtained in three different environmental conditions using functional and structural means.…”