Diversity of Timing Systems in Cyanobacteria and Beyond

Schmelling, Nicolas; Scheurer, Nina; Köbler, Christin; Wilde, Annegret; Axmann, Ilka M.

doi:10.1007/978-3-030-72158-9_10

Cited by 4 publications

(4 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In S. elongatus , there is a single kaiABC clock gene cluster which encodes the central core clock proteins mediating the clockwork ( Ishiura et al, 1998 ; Johnson et al, 2017 ), but in Synechocystis , there are three kaiC homologs organized with and without kaiA and kaiB homologs ( Figure 2A ) ( Aoki & Onai, 2009 ). When the presence of kaiC homologs among bacterial species is assessed globally, it is not unusual to find species with two or more kaiC homologs ( Aoki & Onai, 2009 ; Schmelling et al, 2021 ). Since S. elongatus is able to elaborate a precise clockwork with only one kaiC homolog, what is the function of multiple kaiC genes in those species that harbor more than one copy?…”

Section: Resultsmentioning

confidence: 99%

“…In hindsight, it was fortuitous that the first cyanobacterial species to be analyzed genetically for circadian rhythmicity was S. elongatus rather than Synechocystis ( Kondo et al, 1993 ; Kondo et al, 1994 ; Ishiura et al, 1998 ). First, S. elongatus has a single kaiABC cluster ( Ishiura et al, 1998 ), whereas Synechocystis and many other cyanobacterial species have multiple copies/clusters of kai genes that might have complicated the initial analysis of the genetic basis of the circadian clockwork ( Aoki & Onai, 2009 ; Schmelling et al, 2021 ). Second, the first luciferase reporter created in S. elongatus for other experimental goals, the P psbAI :: luxAB reporter, turned out to exhibit excellent high-amplitude rhythms ( Kondo et al, 1993 ) that were optimal for enlistment to the high-throughput screen for clock mutations ( Kondo et al, 1994 ) that ultimately led to the discovery of the kaiABC clock gene cluster ( Ishiura et al, 1998 ).…”

Section: Discussionmentioning

confidence: 99%

“…However, in comparison with S. elongatus , Synechocystis has been understudied in terms of its circadian properties ( Aoki & Onai, 2009 ; Schmelling et al, 2021 ). The analysis of circadian phenomena in Synechocystis may have been hampered by the fact that the P dnaK ::luxAB reporter developed for Synechocystis ( Aoki et al, 1995 ) was never as robust as the P psbAI ::luxAB reporter that revolutionized circadian analyses in S. elongatus ( Kondo et al, 1993 ; Kondo et al, 1994 ; Johnson & Xu, 2009 ; Johnson et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Synechocystis: A model system for expanding the study of cyanobacterial circadian rhythms

Zhao

Wang

et al. 2023

Front. Physiol.

View full text Add to dashboard Cite

The study of circadian rhythms in bacteria was transformed by studies of the cyanobacterium Synechococcus elongatus. However, in a number of respects S. elongatus is atypical, and while those unusual characteristics were helpful for rapid progress in the past, another commonly used cyanobacterial species, Synechocystis sp. PCC 6803, may be more representative and therefore more productive for future insights into bacterial clock mechanisms. In the past, circadian studies of Synechocystis have suffered from not having an excellent reporter of circadian gene expression, but we introduce here a new luminescence reporter that rivals the reporters that have been used so successfully in S. elongatus. Using this new system, we generate for the first time in Synechocystis circadian period mutants resulting from point mutations. The temperature compensation and dark-pulse resetting that mediates entrainment to the environment is characterized. Moreover, we analyse the complex organization of clock genes in Synechocystis and identify which genes are essential for circadian rhythmicity and adaptive fitness for entrainment and optimal phase alignment to environmental cycles (and which genes are not). These developments will provide impetus for new approaches towards understanding daily timekeeping mechanisms in bacteria.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Synechocystis: A model system for expanding the study of cyanobacterial circadian rhythms

Zhao

Wang

et al. 2023

Front. Physiol.

View full text Add to dashboard Cite

show abstract

“…The revelation of conserved phosphorylation-dependent signaling pathways highlights the evolutionary importance of protein phosphorylation as a conserved mechanism facilitating metabolic adaptation across diverse microbial taxa [98]. Furthermore, the elucidation of cyanobacteria-specific phosphorylation networks exposes unique adaptations tailored to their ecological niches, presenting promising targets for biotechnological applications and environmental engineering [99].…”

Section: Protein Phosphorylation: a Key Player In Metabolic Integrationmentioning

confidence: 99%

Protein Phosphorylation Nexus of Cyanobacterial Adaptation and Metabolism

Nawaz,

Fahad,

Zhou

2024

Kinases and Phosphatases

View full text Add to dashboard Cite

Protein phosphorylation serves as a fundamental regulatory mechanism to modulate cellular responses to environmental stimuli and plays a crucial role in orchestrating adaptation and metabolic homeostasis in various diverse organisms. In cyanobacteria, an ancient phylum of significant ecological and biotechnological relevance, protein phosphorylation emerges as a central regulatory axis mediating adaptive responses that are essential for survival and growth. This exhaustive review thoroughly explores the complex terrain of protein phosphorylation in cyanobacterial adaptation and metabolism, illustrating its diverse forms and functional implications. Commencing with an overview of cyanobacterial physiology and the historical trajectory of protein phosphorylation research in prokaryotes, this review navigates through the complex mechanisms of two-component sensory systems and their interplay with protein phosphorylation. Furthermore, it investigates the different feeding modes of cyanobacteria and highlights the complex interplay between photoautotrophy, environmental variables, and susceptibility to photo-inhibition. The significant elucidation of the regulatory role of protein phosphorylation in coordinating light harvesting with the acquisition of inorganic nutrients underscores its fundamental importance in the cyanobacterial physiology. This review highlights its novelty by synthesizing existing knowledge and proposing future research trajectories, thereby contributing to the deeper elucidation of cyanobacterial adaptation and metabolic regulation through protein phosphorylation.

show abstract