Diversity of Thermophiles in Terrestrial Hot Springs of Yunnan and Tibet, China

Xian, Wen-Dong; Rao, Manik Prabhu Narsing; Zhou, En‐Min; Liu, Lan; Xiao, Min; Li, Wenjun

doi:10.1007/978-981-13-0329-6_3

Cited by 6 publications

(6 citation statements)

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“…Microorganisms exhibiting optimal growth and multiplication at a temperature above 60°C are considered true thermophiles. Their relentless adaptation to survival physiology and ability to thrive under extreme conditions leads to genomic evolution ( Xian et al, 2018 ). The resultant abundance of genes necessary for survival strategies was predicted to be functional in high-temperature spring sample DJ03 (65°C).…”

Section: Discussionmentioning

confidence: 99%

“…The resultant abundance of genes necessary for survival strategies was predicted to be functional in high-temperature spring sample DJ03 (65°C). DNA repair and replication genes and pathways are the adaptive features of microorganisms responsible for taking care of DNA damage due to extreme temperature ( Xian et al, 2018 ). These thermophiles are highly evolved and thrive against the environment’s deleterious effects compared to mesophiles ( Li et al, 2014 ; Saxena et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

“…These thermophiles are highly evolved and thrive against the environment’s deleterious effects compared to mesophiles ( Li et al, 2014 ; Saxena et al, 2016 ). A high abundance of translational machinery directs a higher percentage of A + G in mRNA, making the thermophiles more stable and protects them from adverse environments ( Xian et al, 2018 ). The dominance of energy metabolism pathways in the 65°C spring sample is another tactic followed by the thermophiles used to counter the heat stress as a physiological response, which is further supported by previous reports of the upregulation of Glycolytic pathway-related proteins Geobacillus sp.…”

Section: Discussionmentioning

confidence: 99%

“…The dominance of energy metabolism pathways in the 65°C spring sample is another tactic followed by the thermophiles used to counter the heat stress as a physiological response, which is further supported by previous reports of the upregulation of Glycolytic pathway-related proteins Geobacillus sp. ( Xian et al, 2018 ). Despite the adapted survivability strategies of the dwellers of spring DJ03, it shows low species richness, which indicates that a smaller portion of the microbiome acclimatizes in these extreme conditions.…”

Section: Discussionmentioning

confidence: 99%

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Bacterial Diversity and CAZyme Potential Revealed in Pandanus Rich Thermal Spring Cluster of India: A Non-cultivable 16S rRNA Sequencing Approach

et al. 2021

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In the present study, we explored four different geothermal spots of the Deulajhari spring cluster at a proximity of 10–20 meters with temperatures of 43 to 65°C to unravel their genesis, bacterial diversity and CAZyme potential. However, minor variations in physicochemical properties; TOC, sodium, chloride, zinc and nitrate were observed, including the pH of the spring openings. Illumina based amplicon sequencing revealed Firmicutes, Proteobacteria and Chloroflexi as the major bacterial phylum with higher abundance in the DJ04 sample. The alpha diversity of all the springs was almost same, whereas beta diversity revealed variations in the degree of uniqueness of OTUs at different temperatures. Statistical analysis established a positive correlation between sulfur content with Heliobacterium, Thermodesulfovibrio, Thermodesulfobacterium and Herpetosipho as well as TOC and HCO3 with Thermoanaerobacter, Desulfovibrio, Candidatus solibacter and Dehalogenimona. The major hydrocarbon family genes and Carbohydrate Active Enzyme pathways were predicted to be highest in DJ04 with elevated concentrations of HCO3 and TOC. Higher homogeneity in geo-physicochemical and microbial features direct the possibility of the common origin of these springs through plumbing systems. However, the minor variations in diversity and functionality were due to variations in temperature in spring openings through the mixing of subsurface water contaminated with carbohydrates from leaf biomass litter. Functional characterization of the thermophilic bacteria of this spring provides essential scope for further industrial applications. The biogeochemical reasons hypothesized for the genesis of unique multiple openings in the cluster are also of interest to conservation scientists for taking measures toward necessary laws and regulations to protect and preserve these springs.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Bacterial Diversity and CAZyme Potential Revealed in Pandanus Rich Thermal Spring Cluster of India: A Non-cultivable 16S rRNA Sequencing Approach

et al. 2021

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“…China is a hotspot for hot springs and the Geothermal Professional Committee of the China Energy Research Society in 1986 has identified nearly 3398 hot springs distributed across PR China and most of them are located in Yunnan and Tibet [1]. The hot springs of Yunnan Province have unique physical and chemical features, and in the past few years, we have made an attempt to understand the microbial diversity of Tengchong (Yunnan province, south-west PR China) hot springs.…”

Section: Full-textmentioning

confidence: 99%

Description of Paenibacillus tepidiphilus sp. nov., isolated from a tepid spring

Rao

Dong

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

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China is a hotspot for hot springs and during microbial diversity analysis of Tengchong hot spring, Yunnan province, south-west PR China, two strains designated SYSU G01001T and SY-13 were isolated. SYSU G01001T and SY-13 were Gram-stain-positive, motile and spore-forming. Colonies were white, circular, raised and punctiform. SYSU G01001T and SY-13 grew at pH 6.0–9.0 (optimum pH 8.0) and at 23–37 °C (optimum 28 °C). The 16S rRNA gene sequence similarity between SYSU G01001T and SY-13 was 99.6 % but these strains shared low sequence similarity with Paenibacillus azotifigens (97.5 %) indicating that they represented a novel species. On the basis of the results, SYSU G01001T was selected for further investigations and SY-13 was considered to represent a second strain of the species. The cell wall peptidoglycan of SYSU G01001T was meso-2,6-diaminopimelic acid and MK-7 was the only respiratory quinone. The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified aminolipids (AL), two unidentified amino phospholipids (APL), an unidentified phospholipid (PL) and an unidentified polar lipid (L). The G+C content of the genomic DNA was 53.9 mol%. The average nucleotide identity (ANIb and ANIm) values between SYSU G01001T and Paenibacillus azotifigens LMG 29963T were below the cut-off level (95–96 %) recommended as the average nucleotide identity (ANI) criterion for interspecies identity. On the basis of the above results strain SYSU G01001T represents a novel species of the genus Paenibacillus , for which the name Paenibacillus tepidiphilus sp. nov. is proposed. The type strain is SYSU G01001T (=KCTC 33952T=CGMCC 1.13870T).

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Taxonomic Characteristics of Dominant Microbial Communities in Hot Spring Sediments in Western Georgia

Geliashvili

Jaiani

Тедиашвили

et al. 2021

Microorganisms for Sustainability

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Diversity of Thermophiles in Terrestrial Hot Springs of Yunnan and Tibet, China

Cited by 6 publications

References 66 publications

Bacterial Diversity and CAZyme Potential Revealed in Pandanus Rich Thermal Spring Cluster of India: A Non-cultivable 16S rRNA Sequencing Approach

Bacterial Diversity and CAZyme Potential Revealed in Pandanus Rich Thermal Spring Cluster of India: A Non-cultivable 16S rRNA Sequencing Approach

Description of Paenibacillus tepidiphilus sp. nov., isolated from a tepid spring

Taxonomic Characteristics of Dominant Microbial Communities in Hot Spring Sediments in Western Georgia

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