Description of Paenibacillus tepidiphilus sp. nov., isolated from a tepid spring

Rao, Manik Prabhu Narsing; Dong, Zhou-Yan; Yu, Kuo-Tsong; Dong, Lei; Li, Shuai; Xiao, Min; Yang, Kang; Zhang, Kun; Li, Wenjun

doi:10.1099/ijsem.0.004004

Cited by 42 publications

(19 citation statements)

References 41 publications

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“…Growth was determined at various temperatures (20, 30, 40, 50, 60 and 70 °C) and pH levels (pH 5.0, 6.0, 7.0, 8.0, 9.0, and 10.0). The pH of the PCA medium was adjusted using the buffer system described by Narsing Rao et al [30]. Tolerance to NaCl was tested on International Streptomyces Project 2 agar at concentrations of 1–10 % (w/v).…”

Section: Full-textmentioning

confidence: 99%

Brevibacillus composti sp. nov., isolated from hyperthermophilic compost

Tang

Zhang

Yin

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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Two aerobic, Gram-stain-positive, rod-shaped, endospore-forming, thermophilic bacterial strains, designated FJAT-54423T and FJAT-54424, were isolated from hyperthermophilic compost sampled in Shanxi Province, PR China. Growth was observed at 30–60 °C (optimum, 50 °C) and pH 6.0–9.0 (optimum, pH 7.0), with up to 2.0 % (w/v) NaCl (optimum, 0 % NaCl). The 16S rRNA gene sequence similarity between FJAT-54423T and FJAT-54424 was 99.9%, and the maximum similarity to a valid taxon was observed with Brevibacillus borstelensis (98.3%). Further, in phylogenetic and phylogenomic trees, strains FJAT-54423T and FJAT-54424 branched with members of the genus Brevibacillus . The menaquinone was MK-7, and the major fatty acids were iso-C15 : 0 and anteiso-C15 : 0. The main polar lipids included phosphatidylmethylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylglycerol. The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid. The DNA G+C content of strains FJAT-54423T and FJAT-54424 were 54.3 and 54.4 mol%, respectively. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values of strain FJAT-54423T and its most closely related reference strain B. borstelensis DSM 6347T were 77.7 and 21.5 %, respectively, which were lower than the recommended species delineation thresholds of ANI (95%) and dDDH (70%). Based on the observed physiological properties, chemotaxonomic characteristics and ANI and dDDH values, FJAT-54423T and FJAT-54424 belong to a novel species of the genus Brevibacillus , for which the name Brevibacillus composti sp. nov. is proposed. The type strain is FJAT-54423T (=GDMCC 1.2054T=KCTC 43273T).

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Section: Full-textmentioning

confidence: 99%

Brevibacillus composti sp. nov., isolated from hyperthermophilic compost

Tang

Zhang

Yin

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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“…Genomic DNA extraction and 16S rRNA gene amplification, purification, cloning and sequencing of the raw sequence was carried out as described previously [15]. The obtained sequence was compared with the 16S rRNA gene sequences of species with validly published species in EzBioCloud server (www.ezbiocloud.net) [16].…”

Section: Full-textmentioning

confidence: 99%

Pontibacter pamirensis sp. nov., isolated from saline-alkaline soil

Liu

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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A novel bacterium, designated TRT317T, was isolated from saline-alkaline soil collected from the Pamir plateau in northwest China. Cells of this strain were Gram-stain-negative, aerobic rods and red-pink-coloured. Phylogenetic analysis using 16S rRNA gene sequences indicated that strain TRT317T showed the highest sequence similarity to the type strains of Pontibacter diazotrophicus (96.3 %) and Pontibacter yuliensis (96.2 %). Growth was observed at 4–40 °C, pH 6.0–10.0 and in the presence of up to 7 % (w/v) NaCl. The major fatty acids were iso-C15 : 0 and summed feature 4 (iso-C17 : 1 I/anteiso-C17 : 1 B). The polar lipids included phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unidentified phospholipid, four unidentified glycolipids and five unidentified lipids. The whole-cell sugars of strain TRT317T were mannose, rhamnose, glucose, galactose, xylose, arabinose and four unidentified sugars. The sole respiratory quinone was MK-7. The genomic DNA G+C content of strain TRT317T was 47.7 mol%. The average nucleotide identity (ANI) value of strain TRT317T with P. diazotrophicus was 88.3 %, which is below the standard ANI threshold for species identification (95–96 %). Combined results of physiological, genotypic, phylogenetic and chemotaxonomic analyses demonstrated that strain TRT317T represents a novel species within the genus Pontibacter , for which the name Pontibacter pamirensis sp. nov. is proposed. The type strain is TRT317T (=CGMCC1.18690T=KCTC 82818T).

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“…It indicated that the bacterial strain investigated in this study formed a distinct monophyletic clade from the genus Paenibacillus . Strain S150 T shared low sequence similarity with the type strains of: P. jilunlii Be17 GQ985393 T (97.5%) [14], P. sonchi X19-5 T DQ358736 (97.2%) [15], P. tepidiphilus SYSU G01001 T (96.7%) [16], suggesting that strain S150 T should be classified as a novel species in the genus Paenibacillus .…”

Section: Figurementioning

confidence: 99%

Paenibacillus xylinteritussp. nov., a novel bacterial species isolated from grapevine wood

Haidar

Antonielli

Compant

et al. 2022

Preprint

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Bacteria naturally colonize grapevine wood, and some populations interact synergistically with fungal pathogens to promote wood degradation. In this study, we characterized a bacterial strain, designated S150T, isolated from the wood of grapevine cultivar Sauvignon Blanc. The strain was previously known as promoting wood degradation caused by the causal agent of white-rot, Fomitiporia mediterranea. Phylogenetic analysis based on the full-length 16S rRNA gene sequence showed that the strain S150T belonged to the genus Paenibacillus. Based on 16S rRNA gene sequences, S150T shared low sequence similarity values (between 96.5 and 97.5%) with closed recognized members of the genus (P. jilunlii Be17, P. tepidiphilus SYSU G01001, P. tengchongensis SYSU-G-01003, P. sonchi X19-5, P. helianthi P26E). The cell wall peptidoglycan of S150 contains meso-diaminopimelate. MK6 and MK7 are the two respiratory quinones. The main cellular fatty acids are iso-C16:0 and anteiso-C15:0. The polar lipids are diphosphatidylglycerol, phosphatidylethanolamine, three amino phospholipids, two phospholipids and three polar lipids. The whole-genome is 7.45 Mb, with a G+C content of 52.54%. The average nucleotide identity (ANI) value between S150T and the closely related Paenibacillus member (P. sonchi X19-5) was 85.06%. Biochemical and physiological analyses revealed that strain S150T has different characteristics from reference strains Paenibacillus spp.. Phenotypic, phylogenetic and chemotaxonomic analyses show strain S150T is a novel species of the genus Paenibacillus, for which the name Paenibacillus xylinteritus is proposed.

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Description of Paenibacillus tepidiphilus sp. nov., isolated from a tepid spring

Cited by 42 publications

References 41 publications

Brevibacillus composti sp. nov., isolated from hyperthermophilic compost

Brevibacillus composti sp. nov., isolated from hyperthermophilic compost

Pontibacter pamirensis sp. nov., isolated from saline-alkaline soil

Paenibacillus xylinteritussp. nov., a novel bacterial species isolated from grapevine wood

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