Diversity of sponge-associated fungi from a mangrove forest in Kemujan Island, Karimunjawa National Park, Indonesia

Sibero, Mada Triandala; Pribadi, Rudhi; Larasati, Stefanie Jessica Henny; Calabon, Mark S.; Sabdono, Agus; Subagiyo, Subagiyo; Frederick, E H

doi:10.13057/biodiv/d221256

Cited by 5 publications

(5 citation statements)

References 44 publications

(66 reference statements)

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“…These orders have been found in different sponges around the world and might occupy important functions in the sponge holobiont (Yu et al, 2013;He et al, 2014;Bovio et al, 2019;Karthik and Li, 2019). The genera Aspergillus and Penicillium have often been recovered from marine sponges and macroalgae (Venkatachalam et al, 2015;Yang et al, 2018;Moreno-Pino et al, 2020;Sibero et al, 2021) and our study reports Aspergillus in the sponge Biemna sp. and that of Penicillium in the brown algae Turbinaria conoides.…”

Section: Discussionsupporting

confidence: 61%

Metabarcoding assessment of fungal diversity in brown algae and sponges of Mauritius

et al. 2022

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Marine fungi are largely associated with second most inhabitants of the marine ecosystem such as sponges and algae. They are important colonizers and play vital ecological roles, such as nutrient cycling, organic matter decomposition, and symbiosis with other organisms. High throughput sequencing methods have been used successfully to reveal unknown fungal communities associated with a number of hosts particularly in the marine environment. However, the diversity of marine fungi associated with sponges and brown algae in Mauritius remains largely unknown. Traditional methods based on culturing do not provide reliable estimate of fungal diversity as only those that are able to grow under laboratory conditions are dominant; in addition, a large proportion of fungi, cultured in vitro remain most of the time unidentifiable, given that there are no sporulating structures to be examined morphologically. To overcome these limitations, we employed Illumina sequencing to unravel fungi species present in the sponges, Iotrochota sp. and Biemna sp. and the brown algae Turbinaria conoides, Sargassum pfeifferae, and Sargassum obovatum, collected from the north of Mauritius. Diversity analyses revealed that Biemna sp. had the highest diversity from the sampled sponges with fungi from 24 orders being recovered while from brown algae; Turbinaria conoides had the highest diversity with recovery of fungal taxa of the orders Botryosphaeriales, Chaetothyriales, Eurotiales, Hypocreales, and Mucorales with the latter four orders being common in both sampled algae and sponges. Beta diversity analyses revealed clustering only in the algae, Turbinaria conoides, and Sargassum pfeifferae and not in the co-occurring sponges, indicating that sampling location did not have much influence on fungal diversity. Our findings provide the first amplicon sequencing based insights of the fungal communities associated with macro-algae and sponges in Mauritius and supplements research on the fungal community existing in the oceans around the world.

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Section: Discussionsupporting

confidence: 61%

Metabarcoding assessment of fungal diversity in brown algae and sponges of Mauritius

et al. 2022

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“…In addition, Buzzini et al (2017) reported their marine yeasts were classified as facultative marine fungi as well. Marine fungi are suggested to be carried from the terrestrial to the ocean through the biological vector, rain, wind or other natural phenomenon (Sibero et al 2021). They are also equipped with special mechanism to tolerate the environmental conditions, such as morphology regulation, cell anatomy, cation transport entity modulation, and secondary metabolite production (Hohmann 2002;Ariño et al 2010;Nagano et al 2010;Sharma and Sharma 2017).…”

Section: Discussionmentioning

confidence: 99%

First report of seaweed-associated yeast from Indonesia: Species composition and screening of their polysaccharides-degrading enzymes

et al. 2022

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Abstract. Sibero MT, Frederick EH, Sabdono A, Wijayanti DP, Pringgenies D, Radjasa OK, Zilda DS, Murwani R. 2022. First report of seaweed-associated yeast from Indonesia: Species composition and screening of their polysaccharides-degrading enzymes. Biodiversitas 23: 1408-1419. Yeast has been widely utilized in various industries due to its enzyme properties. Therefore, plenty of studies focus on exploring yeast biodiversity from diverse sources. However, there are limited reports regarding marine yeast biodiversity and its potential from Indonesia. This study aimed to isolate and identify the seaweed-associated yeast from Jepara in Central Java and Sepanjang Beach in Gunung Kidul, Yogyakarta, then examined their potential to produce extracellular polysaccharides-degrading enzymes (EPEs). Marine yeasts were isolated using standard marine agar (STD) and potato dextrose agar (PDA). All isolates were characterized by their salinity tolerance, morphology, and species confirmation using DNA barcoding. Three EPEs consisting of agarase, alginate-lyase, and carrageenase were screened using polysaccharides-enriched agar media. A specific agarase encoding gene was detected with specific primers. In total, 21 seaweed-associated yeast were successfully isolated from 6 seaweeds and noted as facultative marine yeast. The DNA barcoding study discovered that these yeasts belonged to 5 genera, consisted of Aureobasidium, Candida, Debaryomyces, Hortaea, and Rhodotorula. Moreover, Candida was noted as the most abundant genus (71.42%). It was noted that Hortaea werneckii MTJ.11 and MTJ.13 were positive for all enzymes; Aureobasidium melanogenum MTGK.31 gave a positive result for agarase and carrageenase; while [Candida] zeylanoides MTGK.23 only exhibited alginate-lyase activity. Unfortunately, none of the primers used to detect the presence of genes encoding polysaccharide-degrading enzymes were successfully amplified.

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“…Species of marine fungi reported in 2021–2022 were Aspergillus spp. [ 115 , 133 ], Aspergillus flavus [ 122 ] , Aspergillus fumigatus [ 122 ], Aspergillus ochraceus, Aspergillus niger , Aspergillus nomius [ 116 ], Aspergillus aculeatus [ 118 ], Penicillium spp. [ 122 , 126 ], Penicillium citrinum [ 118 ], Penicillium oligosporum [ 125 ], Diaporthe stewartii [ 118 ], Diaporthe spp.…”

Section: Indonesian Marine Macro Organismsmentioning

confidence: 99%

Indonesian marine and its medicinal contribution

Nugraha,

Firli,

Rani

et al. 2023

Nat. Prod. Bioprospect.

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The archipelagic country of Indonesia is populated by the densest marine biodiversity in the world which has created strong global interest and is valued by both Indigenous and European settlements for different purposes. Nearly 1000 chemicals have been extracted and identified. In this review, a systematic data curation was employed to collate bioprospecting related manuscripts providing a comprehensive directory based on publications from 1988 to 2022. Findings with significant pharmacological activities are further discussed through a scoping data collection. This review discusses macroorganisms (Sponges, Ascidian, Gorgonians, Algae, Mangrove) and microorganism (Bacteria and Fungi) and highlights significant discoveries, including a potent microtubule stabilizer laulimalide from Hyattella sp., a prospective doxorubicin complement papuamine alkaloid from Neopetrosia cf exigua, potent antiplasmodial manzamine A from Acanthostrongylophora ingens, the highly potent anti trypanosomal manadoperoxide B from Plakortis cfr. Simplex, mRNA translation disrupter hippuristanol from Briareum sp, and the anti-HIV-1 (+)-8-hydroxymanzamine A isolated from Acanthostrongylophora sp. Further, some potent antibacterial extracts were also found from a limited biomass of bacteria cultures. Although there are currently no examples of commercial drugs from the Indonesian marine environment, this review shows the molecular diversity present and with the known understudied biodiversity, reveals great promise for future studies and outcomes. Graphical Abstract

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Diversity of sponge-associated fungi from a mangrove forest in Kemujan Island, Karimunjawa National Park, Indonesia

Cited by 5 publications

References 44 publications

Metabarcoding assessment of fungal diversity in brown algae and sponges of Mauritius

Metabarcoding assessment of fungal diversity in brown algae and sponges of Mauritius

First report of seaweed-associated yeast from Indonesia: Species composition and screening of their polysaccharides-degrading enzymes

Indonesian marine and its medicinal contribution

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