Diversity of serotypes and new cps loci variants among Streptococcus suis isolates from pigs in Poland and Belarus

Bojarska, Agnieszka; Janas, Katarzyna; Pejsak, Z.; Otulak, Katarzyna; Garbaczewska, Grażyna; Hryniewicz, Waleria; Sadowy, Ewa

doi:10.1016/j.vetmic.2019.108534

Cited by 17 publications

(13 citation statements)

References 24 publications

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“…This Gram‐positive bacterium is also considered as a severe zoonotic and foodborne pathogen (Ferrando et al., 2015). S. suis currently includes 29 recognized serotypes based on the antigenicity of capsular polysaccharides (CPS) and 28 novel cps loci (NCL) (Chz, NCL1‐26 and NCL21') (Bojarska et al., 2020; Huang et al., 2019; Pan et al., 2015; Zheng et al., 2017). At least ten serotypes have been reported that can cause human infections, consisting of serotypes 2, 4, 5, 7, 9, 14, 16, 21, 24 and 31 (Liang et al., 2021).…”

Section: Introductionmentioning

confidence: 99%

The characteristics of population structure and antimicrobial resistance of Streptococcus suis serotype 8, a non‐negligible pathotype

Liu

Liang

et al. 2022

Transbounding Emerging Dis

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Streptococcus suis, the leading causative agent of swine streptococcosis, is considered as a severe zoonotic and foodborne pathogen for humans. Characteristics of population structure and pathogenicity of S. suis vary significantly by serotypes. As one of the main pathogenic serotypes causing clinical disease in pigs, very little is known about the pathogenicity, population structure, and antimicrobial resistance of S. suis serotype 8 (SS8). In this study, the genome of 26 SS8 strains isolated from healthy and diseased pigs was sequenced. Together with 38 sequences from NCBI, we found that SS8 population was clustered into 12 sequence types (ST) and 4 minimum core genome (MCG) groups, linked to the geographical distribution. Noteworthily, 10 strains belonged to MCG group 1 which was defined to possess the capacity to cause global outbreaks in our previous study. We found that 75% (9/12) of representative SS8 strains were virulent in mice and zebrafish, including all ST1241 strains. No virulence indicators were identified from 67 putative virulence‐associated genes mainly identified among pathogenic serotype 2 strains. Instead, we found that the genotype of some of these genes was correlated to their evolution. All 26 isolates were classified as multidrug‐resistant strains by antimicrobial susceptibility testing. The high carrying rate of tetO and ermB, mainly disseminated by integrative mobilizable elements, contributed to the prevalent resistance phenotypes to macrolides, lincosamides and tetracyclines. These findings indicated that the pathogenic potential of SS8 cannot be ignored and provided valuable information for SS8 surveillance.

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Section: Introductionmentioning

confidence: 99%

The characteristics of population structure and antimicrobial resistance of Streptococcus suis serotype 8, a non‐negligible pathotype

Liu

Liang

et al. 2022

Transbounding Emerging Dis

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“…To date, serotyping is an important routine diagnostic procedure and is widely used for subtyping S. suis strains. Among 29 confirmed serotypes (1–19, 21, 23–25, 27–31, and 1/2) and 28 novel cps types [ 2–5 ], serotype 2 is most frequently isolated from clinical cases in swine and humans worldwide [ 1 , 6 ]. Two outbreaks featured by high rates of streptococcal toxic-shock-like syndrome (STSLS) were caused by S. suis serotype 2 sequence type (ST)7 strains in China [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Genomic and pathogenic investigations of Streptococcus suis serotype 7 population derived from a human patient and pigs

Liang

Wang

Gottschalk

et al. 2021

Emerging Microbes & Infections

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Streptococcus suis is one of the important emerging zoonotic pathogens. Serotype 2 is most prevalent in patients worldwide. In the present study, we first isolated one S. suis serotype 7 strain GX69 from the blood culture of a patient with septicemia complicated with pneumonia in China. In order to deepen the understanding of S. suis serotype 7 population characteristics, we investigated the phylogenetic structure, genomic features, and virulence of S. suis serotype 7 population, including 35 strains and 79 genomes. Significant diversities were revealed in S. suis serotype 7 population, which were clustered into 22 sequence types (STs), five minimum core genome (MCG) groups, and six lineages. Lineages 1, 3a, and 6 were mainly constituted by genomes from Asia. Genomes of Lineages 2, 3b, and 5a were mainly from Northern America. Most of genomes from Europe (41/48) were clustered into Lineage 5b. In addition to strain GX69, 13 of 21 S. suis serotype 7 representative strains were classified as virulent strains using the C57BL/6 mouse model. Virulence-associated genes preferentially present in highly pathogenic S. suis serotype 2 strains were not suitable as virulence indicators for S. suis serotype 7 strains. Integrative mobilizable elements were widespread and may play a critical role in disseminating antibiotic resistance genes of S. suis serotype 7 strains. Our study confirmed S. suis serotype 7 is a non-negligible pathotype and deepened the understanding of the population structure of S. suis serotype 7, which provided valuable information for the improved surveillance of this serotype.

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“…In comparison with the serotype distribution in other countries, serotype 2 is the most prevalent in China, Japan, Vietnam, Thailand, Spain, Italy, France, Poland and White Russia 20 . The second prevalent serotype varies with countries, which are mostly serotypes 3 and 4 in Korea, serotypes 3, 1/2 and 7 in the U.S., serotype 9 in the Netherlands, and serotypes 1 and 14 in Britain 21 , 22 . Our result is close to the data reported in the U.S., in which serotypes 3 and 7 are more prevalent.…”

Section: Discussionmentioning

confidence: 99%

Serotype and multilocus sequence typing of Streptococcus suis from diseased pigs in Taiwan

Chen

Chou

et al. 2023

Sci Rep

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Streptococcus suis (S. suis) infection can cause clinically severe meningitis, arthritis, pneumonia and septicemia in pigs. To date, studies on the serotypes, genotypes and antimicrobial susceptibility of S. suis in affected pigs in Taiwan are rare. In this study, we comprehensively characterized 388 S. suis isolates from 355 diseased pigs in Taiwan. The most prevalent serotypes of S. suis were serotypes 3, 7 and 8. Multilocus sequence typing (MLST) revealed 22 novel sequence types (STs) including ST1831-1852 and one new clonal complex (CC), CC1832. The identified genotypes mainly belonged to ST27, ST94 and ST1831, and CC27 and CC1832 were the main clusters. These clinical isolates were highly susceptible to ceftiofur, cefazolin, trimethoprim/sulfamethoxazole and gentamicin. The bacteria were prone to be isolated from cerebrospinal fluid and synovial fluid in suckling pigs with the majority belonging to serotype 1 and ST1. In contrast, ST28 strains that corresponded to serotypes 2 and 1/2 were more likely to exist in the lungs of growing-finishing pigs, which posted a higher risk for food safety and public health. This study provided the genetic characterization, serotyping and the most current epidemiological features of S. suis in Taiwan, which should afford a better preventative and treatment strategy of S. suis infection in pigs of different production stages.

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Diversity of serotypes and new cps loci variants among Streptococcus suis isolates from pigs in Poland and Belarus

Cited by 17 publications

References 24 publications

The characteristics of population structure and antimicrobial resistance of Streptococcus suis serotype 8, a non‐negligible pathotype

The characteristics of population structure and antimicrobial resistance of Streptococcus suis serotype 8, a non‐negligible pathotype

Genomic and pathogenic investigations of Streptococcus suis serotype 7 population derived from a human patient and pigs

Serotype and multilocus sequence typing of Streptococcus suis from diseased pigs in Taiwan

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