Diversity of rotavirus serotypes in Mexican infants with gastroenteritis

Abstract

One hundred thirty-two stool specimens from infants with rotavirus gastroenteritis hospitalized in two Mexican cities (Mexico City and Mérida) were examined by serotype-and subgroup-specific enzyme immunoassays. Among them, 38 (29%) were serotype 1, 15 (11%) were serotype 2, 13 (10%) were serotype 3, 22 (17%) were serotype 4, none was serotype 5 or 6, and 44 (33%) could not be serotyped. By subgrouping, 121 specimens were characterized as follows: 24 (18%) were subgroup 1, 97 (74%) were subgroup 2, and none had both subgroup specificities. While serotype 1 rotavirus predominated in the Mexico City area for 4 consecutive years (1984 to 1987), serotype 4 predominated in Mérida during the single epidemic season studied (1985). These data demonstrate that all four primary human rotavirus serotypes circulated in Mexico, with serotype 1 being the most prevalent. The seroneutralization responses of 14 of the 22 patients infected with serotype 4 strains had been previously studied. Of these 14 infants, 11 appeared to have primary infections, as indicated by absence of neutralizing antibodies in the acute-phase sera and their young age (8 months on average) at the time of illness. Seven patients seroresponded to serotypes 1 and 4; two seroresponded to serotypes 1, 3, and 4; three seroresponded to serotype 1; and two had low-level seroresponses to serotype 3 or 4. These data indicate that heterotypic neutralizing antibody responses occur frequently following infection with serotype 4 rotaviruses.

Antigenic and genetic characterization of serotype G2 human rotavirus strains from South Africa from 1984 to 1998

“…Iturriza-Gómara et al [2001] detected an amino acid substitution at position 96 (Asp ! Asn) likely to be responsible for failure to type G2 strains with G2specific monoclonal antibodies S2-2G10 [Taniguchi et al, 1987], RV5:3 [Coulson et al, 1987], and 1C10 [Padilla-Noriega et al, 1990]. Similarly to Iturriza Gómara's results, two strains (405GR/87 and 514GR/ 87) displaying identical amino acid sequences in antigenic regions A, B, and C to the British 1991 isolates reacted with both G2-specific monoclonal antibodies (Fig.…”

“…G (VP7) Serotyping ELISA VP7 serotyping was completed according to the method described by Taniguchi et al [1987]. The following G2-specific capture monoclonal antibodies were used S2-2G10 [Taniguchi et al, 1987] and IC10 [Padilla-Noriega et al, 1990]. Mab-60 [Shaw et al, 1985] was also included as a positive control to detect the presence of the intact double-shelled viral particles.…”

Rotaviruses: Immunological Determinants of Protection Against Infection and Disease

“…Sera from animals parenterally inoculated with human rotaviruses have been used to define at least nine different human rotavirus G types (G types 1-4, 6, 8, 9, 10, and 12). G types 1-4 are the most important G types isolated in epidemiological studies in both developed and developing countries; G type 1 is the most common (Flores et al, 1988;Matson et al, 1990b;Padilla-Noriega et al, 1990;Bishop et al, 1991;Bingnan et al, 1991;Ahmed et al, 1991;Noel et al, 1991;Woods et al, 1992). Hybridization analysis has been used to distinguish at least five human rotavirus P types Gorziglia et al, 1990;Estes and Cohen, 1989).…”

Classical and molecular techniques for the diagnosis of viral gastroenteritis

“…The epidemiology of rotavirus group A infections in man is complex: at any one time and site there is co-circulation of rotaviruses of different G types, types G1-G4 representing 95% of co-circulating strains worldwide and type GI approximately 50% (Flores et al, 1988;Desselberger, 1989;Beards et al, 1989;Got/vea et al, 1990b;Matson et al, 1990;Padilla-Noriega et al, 1990;Noel et al, 1991). In one country, different serotypes can show regional differences (Noel et al, 1991).…”