Diversity of OspA and OspC among cerebrospinal fluid isolates of Borrelia burgdorferi sensu lato from patients with neuroborreliosis in Germany

Thomssen, Henrike; Hoffmann, Boris; Schank, Marion; Elewaut, Dirk; Büschenfelde, Karl‐Hermann Meyer zum; Märker‐Hermann, E.

doi:10.1007/s004300050007

Cited by 68 publications

(101 citation statements)

References 26 publications

(35 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…BbN40 is a North American tickisolate, 15 Bb297 is a CSF isolate from a patient in Connecticut. 16 BgPbi and BgPli are CSF isolates from Europe, 17 and Bg793 is a tick isolate from Europe (a gift from Martin Schriefer, Division of Vector-Borne Infectious Disease, Center for Disease Control and Prevention, Fort Collins, CO, USA).…”

Section: Borrelia Strainsmentioning

confidence: 99%

Spinal cord involvement in the nonhuman primate model of Lyme disease

Bai

Narayan

Dail

et al. 2004

Laboratory Investigation

View full text Add to dashboard Cite

Lyme borreliosis is a multisystemic disease caused by infection with various genospecies of the spirochete Borrelia burgdorferi. The organs most often affected are the skin, joints, the heart, and the central and peripheral nervous systems. Multiple neurological complications can occur, including aseptic meningitis, encephalopathy, facial nerve palsy, radiculitis, myelitis, and peripheral neuropathy. To investigate spinal cord involvement in the nonhuman primate (NHP) model of Lyme borreliosis, we inoculated 25 adult Macaca mulatta with B. burgdorferi sensu strictu strains N40 by needle (N ¼ 9) or by tick (N ¼ 4) or 297 by needle (N ¼ 2), or with B. burgdorferi genospecies garinii strains Pbi (N ¼ 4), 793 (N ¼ 2), or Pli (N ¼ 4) by needle. Immunosuppression either transiently (TISP) or permanently (IS) was used to facilitate establishment of infection. Tissues and fluids were collected at necropsy 7-24 weeks later. Hematoxylin and eosin staining was used to study inflammation, and immunohistochemistry and digital image analysis to measure inflammation and localize spirochetes. The spirochetal load and C1q expression were measured by TaqMan RT-PCR. The results showed meningoradiculitis developed in only one of the 25 NHP's examined, TISP NHP 321 inoculated with B. garinii strain Pbi. Inflammation was localized to nerve roots, dorsal root ganglia, and leptomeninges but rarely to the spinal cord parenchyma itself. T cells and plasma cells were the predominant inflammatory cells. Significantly increased amounts of IgG, IgM, and C1q were found in inflamed spinal cord. Taqman RT-PCR found spirochetes in the spinal cord only in IS-NHP's, mostly in nerve roots and ganglia rather than in the cord parenchyma. C1q mRNA expression was significantly increased in inflamed spinal cord. This is the first comprehensive study of spinal cord involvement in Lyme borreliosis.

show abstract

Section: Borrelia Strainsmentioning

confidence: 99%

Spinal cord involvement in the nonhuman primate model of Lyme disease

Bai

Narayan

Dail

et al. 2004

Laboratory Investigation

View full text Add to dashboard Cite

show abstract

“…nov.) that was isolated from a patient with neuroborreliosis in 1984 was included within this species. However, it was noted that strain PBi and closely related strains formed a distinct subtype, outer surface protein A (ospA) type 4, within B. garinii (Wilske et al, 1996). RFLP analysis revealed that strains of this subtype were genetically highly homogeneous (Marconi et al, 1999).…”

mentioning

confidence: 99%

Borrelia bavariensis sp. nov. is widely distributed in Europe and Asia

Margos

Wilske²,

Sing³

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

105

View full text Add to dashboard Cite

Since the original description of Borrelia bavariensis sp. nov. in 2009, additional samples available from humans and ticks from Europe and Mongolia, respectively, have been used to further characterize Borrelia strains belonging to this group of spirochaetes that utilize rodents as reservoir hosts. These investigations suggested the presence of related strains in Europe and Asia and confirmed their status as representing a distinct species. Furthermore, samples that were investigated by researchers from China and Japan confirm the ecological relationship of members of this proposed species with rodents and suggest that it has a wide distribution in Eurasia. Here, we use phylogenetic and genetic distance analyses to validate B. bavariensis sp. nov. as a species within the Borrelia burgdorferi sensu lato species complex. The type strain is PBiT ( = DSM 23469T = BAA-2496T).

show abstract

“…However, some differences in the region of 20-25 kDa proteins (presumably OspC) were observed. Considerable antigenic variations have been documented for Borrelia strains; besides the adaptive mechanisms of the spirochetes that influence protein expression, each strain has its own potential to express a particular protein (Wilske et al 1996, Ruzic-Sabljic et al 2001). According to these results, the antigen obtained could be used in a Western-blot for the diagnosis of Lyme borreliosis in humans.…”

mentioning

confidence: 99%