Evaluation of a modified culture medium for Borrelia burgdorferi sensu lato

Rodríguez, Islay; Lienhard, Reto; Gern, Lise; Veuve, Marie Colette; Jouda, Fatima; Siegrist, Hans H.; Fernández, Carmen; Rodríguez, José

doi:10.1590/s0074-02762007000800017

Cited by 3 publications

(3 citation statements)

References 16 publications

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“…However, after 8-10 days, further growth of the spiral form ceased and spirochetes tended to convert into different morphological forms (data not shown) as described previously 39-[42]. Multiple attempts to revert them back to spiral form were unsuccessful regardless of the use of several different media such as the original BSK-II medium 27, modified Kelly medium (MKP medium) 43, MPM medium 44, and BSK modified medium 45. In addition, several solid BSK-based media 46 and PMR-agar media 43 were similarly tested but likewise were not capable of maintaining spirochetal growth.…”

Section: Resultssupporting

confidence: 67%

Improved Culture Conditions for the Growth and Detection of Borrelia from Human Serum

Sapi¹,

Pabbati²,

Datar³

et al. 2013

Int. J. Med. Sci.

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In this report we present a method to cultivate Borrelia spirochetes from human serum samples with high efficiency. This method incorporates improved sample collection, optimization of culture media and use of matrix protein. The method was first optimized utilizing Borrelia laboratory strains, and later by demonstrating growth of Borrelia from sera from fifty seropositive Lyme disease patients followed by another cohort of 72 Lyme disease patients, all of whom satisfied the strict CDC surveillance case definition for Lyme disease. The procedure resulted in positive cultures in 47% at 6 days and 94% at week 16. Negative controls included 48 cases. The positive identification of Borrelia was performed by immunostaining, PCR, and direct DNA sequencing.

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Section: Resultssupporting

confidence: 67%

Improved Culture Conditions for the Growth and Detection of Borrelia from Human Serum

Sapi¹,

Pabbati²,

Datar³

et al. 2013

Int. J. Med. Sci.

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“…The antimalarial activity of artemisinin might involve endoperoxide activation by free ferrous iron from hemoglobin digestion by malaria parasites ( Wells et al, 2009 ). However, the content of ferrous iron or hemoglobin is very low in the B. burgdorferi culture ( Rodriguez et al, 2007 ), so the activation of endoperoxide might not be the main mechanism of artemisinin activity against B. burgdorferi round bodies. In yeast, artemisinin impairs the membrane structure and causes depolarization of the mitochondrial membrane ( Li et al, 2005 ; Wang et al, 2010 ).…”

Section: Discussionmentioning

confidence: 99%

A Drug Combination Screen Identifies Drugs Active against Amoxicillin-Induced Round Bodies of In Vitro Borrelia burgdorferi Persisters from an FDA Drug Library

et al. 2016

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Although currently recommended antibiotics for Lyme disease such as doxycycline or amoxicillin cure the majority of the patients, about 10–20% of patients treated for Lyme disease may experience lingering symptoms including fatigue, pain, or joint and muscle aches. Under experimental stress conditions such as starvation or antibiotic exposure, Borrelia burgdorferi can develop round body forms, which are a type of persister bacteria that appear resistant in vitro to customary first-line antibiotics for Lyme disease. To identify more effective drugs with activity against the round body form of B. burgdorferi, we established a round body persister model induced by exposure to amoxicillin (50 μg/ml) and then screened the Food and Drug Administration drug library consisting of 1581 drug compounds and also 22 drug combinations using the SYBR Green I/propidium iodide viability assay. We identified 23 drug candidates that have higher activity against the round bodies of B. burgdorferi than either amoxicillin or doxycycline. Eleven individual drugs scored better than metronidazole and tinidazole which have been previously described to be active against round bodies. In this amoxicillin-induced round body model, some drug candidates such as daptomycin and clofazimine also displayed enhanced activity which was similar to a previous screen against stationary phase B. burgdorferi persisters not exposure to amoxicillin. Additional candidate drugs active against round bodies identified include artemisinin, ciprofloxacin, nifuroxime, fosfomycin, chlortetracycline, sulfacetamide, sulfamethoxypyridazine and sulfathiozole. Two triple drug combinations had the highest activity against amoxicillin-induced round bodies and stationary phase B. burgdorferi persisters: artemisinin/cefoperazone/doxycycline and sulfachlorpyridazine/daptomycin/doxycycline. These findings confirm and extend previous findings that certain drug combinations have superior activity against B. burgdorferi persisters in vitro, even when pre-treated with amoxicillin. These findings may have implications for improved treatment of Lyme disease.

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“…Rodríguez et al (2007), after replacing CRML 1066 with 199 medium, compared the growth of different strains of Borrelia in relation to the modified Barbour-Stoenner-Kelly (BSK)-H medium with added amino acids and antibiotics and indicated that the modified medium could function as an alternative growth media for the cultivation of Borrelia strains.…”

mentioning

confidence: 99%

Growth, cysts and kinetics of Borrelia garinii (Spirochaetales: Spirochaetacea) in different culture media

Oliveira

Fonseca

Costa

et al. 2010

Mem. Inst. Oswaldo Cruz

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Evaluation of a modified culture medium for Borrelia burgdorferi sensu lato

Abstract: The aim of the present study was to assess the possible use of a modified medium, prepared

Cited by 3 publications

References 16 publications

Improved Culture Conditions for the Growth and Detection of Borrelia from Human Serum

Improved Culture Conditions for the Growth and Detection of Borrelia from Human Serum

A Drug Combination Screen Identifies Drugs Active against Amoxicillin-Induced Round Bodies of In Vitro Borrelia burgdorferi Persisters from an FDA Drug Library

Growth, cysts and kinetics of Borrelia garinii (Spirochaetales: Spirochaetacea) in different culture media

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