Diversity of medicinal plants used for skincare by Bulungan tribe in North Kalimantan, Indonesia and its melanin biosynthesis inhibition

Abstract

Abstract. Kartina, Batubara I, Kuspradini H, Kusuma IW, Wahyuni WT, Egra S, Matsunaga T. 2022. Diversity of medicinal plants used for skincare by Bulungan tribe in North Kalimantan, Indonesia and its melanin biosynthesis inhibition. Biodiversitas 23: 1246-1253. Indigenous people of the Bulungan tribe in North Kalimantan, Indonesia have been utilizing plants for health purposes, including for skincare such as preventing skin from being dark due to melanin production. This study aimed to evaluate the diversity of medicinal plants utilized by the Bulungan tribe for skincare and determine the melanin biosynthesis inhibition activity. Plants having medicinal uses were investigated through literature studies and interviews with the members of Bulungan tribe in Tanjung Palas Tengah Village. Melanin biosynthesis inhibition was determined by identifying the tyrosinase inhibition potential using commercial tyrosinase enzyme, while intracellular and extracellular melanin biosynthesis production activity was determined using B16 melanoma cells. The results showed at least 19 plant species that have medicinal uses, including for skincare, with some of them having the potential for melanogenesis, both in suppressing and producing melanin. We found a plant sample with melanin suppression potential on B16 melanoma cell, namely Jatropha curcas, at 6.2 µg/ml concentration, which could decrease extracellular melanin content by 18%. Tyrosinase activity with L-tyrosine showed that Cinnamomum burmannii (root) (IC50: 3.4 ?g/mL) showed an insignificant difference with kojic acid, which served as positive control by showing an inhibitory level of (IC50: 0.14 ?g/mL). Based on the data obtained, a pharmacological study is recommended to investigate melanin biosynthesis as a skin whitening agent. We believe that this study can become basic data to discover several active compounds in future studies.

Combination of Extraction and Distillation Red Ginger Rhizome on the Composition of Active Compounds and Tyrosinase Inhibitory Activity

“…Tyrosinase inhibitory activity was evaluated based on the potency of inhibition to monophenolase and diphenolase. The assay was carried out using L-tyrosine and L-DOPA as the substrates, following the method described by Kartina et al [20], and kojic acid was used as a positive control. ELISA plate well reader (Merck Biotec Epoc Spectro UV-Vis) was used for tyrosinase inhibition assay.…”

Antimicrobial activity of plant from educational forest in Universitas Borneo Tarakan

“…In this study, the grouping of screening bacteria into three parts, namely oral disease caused by the bacteria S. sobrinus and S. mutans, which cause bad breath and dental caries [16], In skin diseases caused by S. aureus and P. acne [17], these bacteria generally cause boils, acne, pneumonia, meningitis, and arthritis. The last group is caused by the bacterium S. typhi, which causes typhus and digestive diseases [18].…”

Garcidepsidon B from Garcinia parvifolia: Antimicrobial Activities of The Medicinal Plants from North Kalimantan against Dental Caries and Periodontal Disease Pathogen

“…This tree's fruit is edible, and the young leaves are occasionally consumed as vegetables (11). Locally, they are known as asam kandis (12). The leaves (13), twigs (14), latex (15), and bark (16).…”