Diversity of Fruits

Malik, Arvind; Mushtaq, Rafiya; Bhat, Zulfiqar Ali; Ganie, Mumtaz A.

doi:10.1201/9780429264481-1

Cited by 1 publication

(1 citation statement)

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“…Very high heating (>16 °C/s) and cooling rates (>13 °C/s) can be achieved by providing the maximum rated current to the Peltier device (as shown in Figure ). As opposed to what was disclosed in previous designs, our thermal cycler assembly consists only of inexpensive, commercially available components and it requires no movable parts, as the heat produced by the TEC is passively dissipated by the device’s metal body.…”

Section: Design Process and System Setupmentioning

confidence: 99%

Small-Size Polymerase Chain Reaction Device with Improved Heat Transfer and Combined Feedforward/Feedback Control Strategy

Gregorini

Mikutis

Grass

et al. 2019

Ind. Eng. Chem. Res.

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We report improvements on the heat transfer and on the control strategy of a thermal cycler implemented on a novel device for performing a fast polymerase chain reaction (PCR). The reduction of the thermal mass of the sample holder and the direct contact with the sample allow for a significant reduction of the transition times, while the hybrid feedforward/feedback controller can rely on inexpensive components (actuators, sensors, processor) and still achieve minimal over/undershooting and good temperature stability. The design of the device has been improved by performing transient heat conduction analysis on the highly heat-conductive sample holder and on the solid metal body of the device which rapidly dissipates the excess heat produced during the thermal cycling. In the current setup, the sample holder hosts nine 1 μL samples covered with mineral oil, which can be simultaneously read in real time by the detection module designed in-house and installed on the device. An increase in speed of the PCR amplification was achieved with a reduction of the transition time of 63.8% when compared against a commercial real-time PCR machine. Our work shows that a complete, stand-alone, and ready-to-use quantitative PCR instrument can be fabricated from inexpensive and easily available components and it can achieve fast thermal cycling thanks to a hybrid control strategy.

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Section: Design Process and System Setupmentioning

confidence: 99%