Diversity of Escherichia coli strains isolated from day-old broiler chicks, their environment and colibacillosis lesions in 80 flocks in France

Delannoy, Sabine; Schouler, Catherine; Souillard, Rozenn; Yousfi, Lena; Devendec, Laëtitia Le; Lucas, Camille; Bougeard, Stéphanie; Keïta, Alassane; Fach, Patrick; Galliot, Pascal; Balaine, L.; Puterflam, Julie; Kempf, Isabelle

doi:10.1016/j.vetmic.2020.108923

Cited by 10 publications

(13 citation statements)

References 27 publications

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“…Out of the 32 distinct STs identified in the present study, almost 50% were only identified once. A similar trend has been observed in previous studies [ 32 – 34 ]. The high number of STs only represented by one or two isolates influences the high diversity of STs reported.…”

Section: Discussionsupporting

confidence: 92%

A molecular epidemiological study on Escherichia coli in young chicks with colibacillosis identified two possible outbreaks across farms

Kravik

Kaspersen

Sjurseth

et al. 2023

Vet Res

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Avian pathogenic Escherichia coli (APEC) is the cause of colibacillosis outbreaks in young poultry chicks, resulting in acute to peracute death. The high morbidity and mortality caused by colibacillosis results in poor animal welfare, reduced sustainability and economical loss worldwide. To advance the understanding of the molecular epidemiology, genomic relatedness and virulence traits of APEC, we performed systematic sampling from 45 confirmed colibacillosis broiler flocks with high first week mortality (FWM) during 2018–2021. From these flocks, 219 APEC isolates were whole genome sequenced (WGS) and bioinformatic analyses were performed. The bioinformatic analyses included sequence typing (ST), serotyping, detection of virulence-associated genes (VAGs) and phylogenetic analysis. Our results showed a high prevalence of ST23, ST429 and ST95 among APEC isolates from Norwegian broiler flocks, and identified ST23, ST429, ST117 and ST371 to cause disease more often alone, compared to ST95, ST69 and ST10. Phylogenetic analyses, together with associated metadata, identified two distinct outbreaks of colibacillosis across farms caused by ST429 and ST23 and gave insight into expected SNP distances within and between flocks identified with the same ST. Further, our results highlighted the need for combining two typing methods, such as serotyping and sequence typing, to better discriminate strains of APEC. Ultimately, systematic sampling of APEC from multiple birds in a flock, together with WGS as a diagnostic tool is important to identify the disease-causing APEC within a flock and to detect outbreaks of colibacillosis across farms.

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Section: Discussionsupporting

confidence: 92%

A molecular epidemiological study on Escherichia coli in young chicks with colibacillosis identified two possible outbreaks across farms

Kravik

Kaspersen

Sjurseth

et al. 2023

Vet Res

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“…Somatic antigen characterization allowed for the precise identification of the serogroup in 45% of the strains, with the most recurrent being O2 (21%) and O88 (13%). The first serogroup was one of the most frequently isolated in Europe, along with O1, O5, O8, O18 and O78 [ 12 , 13 , 14 ], and both O2 and O88 were linked to colibacillosis in avian species [ 11 ]. On the other hand, they were less frequent in eastern Asian countries, where the O78 serogroup was dominant [ 8 , 15 ].…”

Section: Discussionmentioning

confidence: 99%

“…In particular, a survey reported that half of the European strains belong to six different serogroups (O1, O2, O5, O8, O18 and O78) [ 12 ]. A study carried out in France identified 12 different serogroups, with the most frequently isolated ones being serogroups O2, O8, O25 and O78 [ 13 ]; likewise, a Greek survey mainly identified O78, O2 and O11 [ 14 ]. Studies from different Asian countries highlight a high prevalence of serogroups O78 and O2 [ 15 , 16 ], but also O1 and O18 displayed a relevant prevalence [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Virulence Factors and Antimicrobial Resistance Profile of Escherichia Coli Isolated from Laying Hens in Italy

Gambi

Rossini

Menandro

et al. 2022

Animals

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Colibacillosis is the most common bacterial disease in the poultry industry. The isolation of Escherichia coli (E. coli) strains with multiple resistance to various classes of antimicrobials has been increasing in recent years. In this study, antimicrobial resistance features, serotyping and the presence of avian pathogenic Escherichia coli (APEC) virulence genes were investigated on a total of 71 E. coli strains isolated during outbreaks of colibacillosis in laying hens. The correlation between these features was evaluated. The most frequently isolated serogroups were O2 and O88. Resistance was often detected with nalidixic acid (49%) and ampicillin (38%), while all strains were sensitive to ceftiofur and florfenicol. Overall, 25% of the isolates showed resistance to at least three or more antimicrobial classes (multidrug-resistant strains), and 56% of the isolates were defined as APEC strains (due to the presence of at least five virulence genes). Correlation between the different parameters (virulence genes, serogroup and antimicrobial resistance) did not reveal relevant associations. The comparison of the obtained results with those of similar studies highlighted the importance of continuous monitoring in order to have a better understanding of colibacillosis. An evaluation of the national epidemiological situation would allow, especially with regard to antimicrobial resistance, to focus on the right measures in order to prioritize the available resources for effective disease control.

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“…Another, more recent paper (Delannoy et al, 2021) evaluated the genetic diversity of E. coli strains isolated from 80 broiler flocks, monitored from before chick placement to colibacillosis outbreaks. The E. coli isolates were characterized using high-throughput qPCR to screen genetic markers related to 23 serogroups, five phylogroups and 66 virulence factors and determine genetic profiles.…”

Section: Introductionmentioning

confidence: 99%

“…Here, we describe two experiments. In the first one, three APEC strains that have been characterized (Delannoy, et al, 2021) in terms of virulence factor content, serogroup and phylogroup were used to develop in vivo models of colibacillosis for DOCs on two chicken breeds. The first one is the ANSES' own Leghorn breed and the second one is the Ross 308 breed (Aviagen company ®), and is the most common broiler breed in Europe.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of passive immunization in broiler chicks via an inactivated Escherichia coli autogenous vaccine administered to broiler breeder hens

Keïta¹,

Devendec²,

Amelot³

et al. 2022

Avian Pathology

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Diversity of Escherichia coli strains isolated from day-old broiler chicks, their environment and colibacillosis lesions in 80 flocks in France

Cited by 10 publications

References 27 publications

A molecular epidemiological study on Escherichia coli in young chicks with colibacillosis identified two possible outbreaks across farms

A molecular epidemiological study on Escherichia coli in young chicks with colibacillosis identified two possible outbreaks across farms

Virulence Factors and Antimicrobial Resistance Profile of Escherichia Coli Isolated from Laying Hens in Italy

Efficacy of passive immunization in broiler chicks via an inactivated Escherichia coli autogenous vaccine administered to broiler breeder hens

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