Diversity of cellulolytic microorganisms and microbial cellulases

Liu, Lirui; Huang, Wen-Cong; Yang, Liting; Li, Meng

doi:10.1016/j.ibiod.2021.105277

Cited by 46 publications

(27 citation statements)

References 189 publications

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“…Interestingly, the enzyme preparation derived from I. lacteus showed a slightly higher cellulose hydrolysis potential than the commercial enzyme. This can be attributed to a more balanced mixture of enzymes and their synergistic interactions [3] as well as to a higher β-glucosidase content, which reduced the inhibitory effect of forming cellobiose. It is worth noting that recently, Mezule and Civzele [19] performed biomass hydrolysis using enzyme preparation obtained from the same strain I. lacteus but reaction mixtures containing 3% w/v of dry biomass (hay, wood or sawing residue chips, barley straw) and 0.2-0.3 FPU/mL were incubated at pH 5.5 and 30 • C. The highest conversion yields were obtained from hay substrate, where more than 20% of the dry matter of biomass has been converted to fermentable sugar within 24 h of incubation.…”

Section: Saccharification Of Pretreated Wheat Straw With An Enzyme Preparation From I Lacteusmentioning

confidence: 99%

“…It is a cheap but complex material consisting of polymers of cellulose, hemicellulose, and lignin; therefore, a wide range of hydrolytic and oxidative enzymes is required for their degradation. Hydrolysis of biomass polysaccharides into fermentable sugars by cellulases and hemicellulases is the key step for enzymatic conversion of lignocellulose [1][2][3][4]. Cellulases comprise endoglucanases (EC 3.2.1.4) which cleave internal β-1,4-glucosidic bonds of cellulose chains, exoglucanases (EC 3.2.1.91) which processively act on the reducing and non-reducing ends of cellulose to release short-chain cello-oligosaccharides, and βglucosidases (EC 3.2.1.21) which hydrolyze soluble cello-oligosaccharides to glucose.…”

Section: Introductionmentioning

confidence: 99%

“…Cellulases are widely used in biofuel, food and feed, textile, paper-pulp, cosmetics, chemicals, and other industries [1,2]. The main challenges for wide and large-scale application of cellulases and xylanases remain the reduction of their cost and the development of more efficient enzyme cocktails with high specific activity and stability [3,4]. Wooddecomposing white-rot basidiomycetes (WRB) are excellent producers of enzymes that deconstruct the cell wall of plants [5].…”

Section: Introductionmentioning

confidence: 99%

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The Carbon Source Controls the Secretion and Yield of Polysaccharide-Hydrolyzing Enzymes of Basidiomycetes

2021

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In the present study, the polysaccharide-hydrolyzing secretomes of Irpex lacteus (Fr.) Fr. (1828) BCC104, Pycnoporus coccineus (Fr.) Bondartsev and Singer (1941) BCC310, and Schizophyllum commune Fr. (1815) BCC632 were analyzed in submerged fermentation conditions to elucidate the effect of chemically and structurally different carbon sources on the expression of cellulases and xylanase. Among polymeric substrates, crystalline cellulose appeared to be the best carbon source providing the highest endoglucanase, total cellulase, and xylanase activities. Mandarin pomace as a growth substrate for S. commune allowed to achieve comparatively high volumetric activities of all target enzymes while wheat straw induced a significant secretion of cellulase and xylanase activities of I. lacteus and P. coccineus. An additive effect on the secretion of cellulases and xylanases by the tested fungi was observed when crystalline cellulose was combined with mandarin pomace. In I. lacteus the cellulase and xylanase production is inducible in the presence of cellulose-rich substrates but is suppressed in the presence of an excess of easily metabolizable carbon source. These enzymes are expressed in a coordinated manner under all conditions studied. It was shown that the substitution of glucose in the inoculum medium with Avicel provides accelerated enzyme production by I. lacteus and higher cellulase and xylanase activities of the fungus. These results add new knowledge to the physiology of basidiomycetes to improve cellulase production.

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Section: Saccharification Of Pretreated Wheat Straw With An Enzyme Preparation From I Lacteusmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Carbon Source Controls the Secretion and Yield of Polysaccharide-Hydrolyzing Enzymes of Basidiomycetes

2021

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“…Recently, the importance of bacterial cellulases has been rising. Cellulolytic enzymes are produced by aerobic and anaerobic bacteria, e.g., bacteria of the genus Bacillus, Butyrivibrio, Cellulomonas, Clostridium, Paenibacillus, and Ruminococcus [13,14]. Of the large number of enzymes produced by resistance to harsh environmental conditions, spore-forming bacteria of the genus Bacillus and related genera seem to be the most interesting [15].…”

Section: Introductionmentioning

confidence: 99%

Cellulolytic Properties of a Potentially Lignocellulose-Degrading Bacillus sp. 8E1A Strain Isolated from Bulk Soil

2022

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Cellulolytic enzymes produced by spore-forming bacteria seem to be a potential solution to the degradation of lignocellulosic waste. In this study, several dozen bacterial spore-forming strains were isolated from soil and one of them was selected for further studies. The studied bacterial strain was identified to genus Bacillus (strain 8E1A) by 16S rRNA gene sequencing. Bacillus sp. 8E1A showed an activity of carboxymethyl cellulase (CMCase) with visualization with Congo Red-25 mm (size of clear zone). To study CMCase, filter paper hydrolase (FPase), and microcrystalline cellulose Avicel hydrolase (Avicelase) production, three different cellulose sources were used for bacterial strain cultivation: carboxymethyl cellulose (CMC), filter paper (FP), and microcrystalline cellulose Avicel. The highest CMCase (0.617 U mL−1), FPase (0.903 U mL−1), and Avicelase (0.645 U mL−1) production of Bacillus sp. 8E1A was noted for using CMC (after 216 h of incubation), Avicel cellulose (after 144 h of incubation), and CMC (after 144 h of incubation), respectively. Subsequently, the cellulases’ activity was measured at various temperatures and pH values. The optimal temperature for CMCase (0.535 U mL−1) and Avicelase (0.666 U mL−1) activity was 70 °C. However, the highest FPase (0.868 U mL−1) activity was recorded at 60 °C. The highest CMCase and Avicelase activity was recorded at pH 7.0 (0.520 and 0.507 U mL−1, respectively), and the optimum activity of FPase was noted at pH 6.0 (0.895 U mL−1). These results indicate that the cellulases produced by the Bacillus sp. 8E1A may conceivably be used for lignocellulosic waste degradation in industrial conditions.

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“…In the new era of technology for the rehabilitation of degraded soils, the role of cellulolytic microorganisms is getting more attention because their enrichment in the soil can accelerate the increase in available nutrients in the growing media, especially nutrients that are absorbed in compounds that are difficult to decompose so that they are difficult to be utilized by plant roots. Lignocellulosic biomass management also has the potential as renewable energy, so that it contributes significantly to increasing energy sources [2,3]. Cellulolytic bacteria and fungi can convert lignocellulosic biomass materials into ethanol by hydrolysis and subsequence fermentation [4,5].…”

Section: Introductionmentioning

confidence: 99%

Comparison of decomposition and adaptation capability of indigenous peat cellulolytic microorganisms

Hafif

Sasmita

Khaerati

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Cellulolytic microorganisms play an essential role in the weathering of lignocellulosic materials. An experiment conducted was to study the potential of indigenous peat cellulolytic microorganisms to decompose peat and adapt to life outside the peat ecosystem. Indigenous cellulolytic bacteria of peat, Comamonas testosteroni, and Delftia lacustris, and indigenous cellulolytic fungi of peat, Penicillium singorense, Aspergillus aculeatus, and Trichoderma sp. were tested their capability for that case. In a greenhouse, each cellulolytic bacteria and fungi colony inoculated to the peat were as treatments and peat without inoculation as control. The study also tested their adaptability to grow in media outside the peat, such as charcoal and zeolite mixed with processed coffee and cacao residue in Lab. The cellulolytic fungi were a little stronger than cellulolytic bacteria in peat decomposition. The fungi reduced the organic C of peat by 13.9%, while the bacteria were only 6.4%. The CO2 flux from peat inoculated by fungi colony, 0.68 mg CO2/kg peat, and by bacteria colony, 0.64 mg CO2/kg peat. Both microbes adapted to the environment outside of peat, especially charcoal and zeolite mixed with coffee and cacao residue. However, cellulolytic bacteria were more robust than cellulolytic fungi if living simultaneously in the same media.

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Diversity of cellulolytic microorganisms and microbial cellulases

Cited by 46 publications

References 189 publications

The Carbon Source Controls the Secretion and Yield of Polysaccharide-Hydrolyzing Enzymes of Basidiomycetes

The Carbon Source Controls the Secretion and Yield of Polysaccharide-Hydrolyzing Enzymes of Basidiomycetes

Cellulolytic Properties of a Potentially Lignocellulose-Degrading Bacillus sp. 8E1A Strain Isolated from Bulk Soil

Comparison of decomposition and adaptation capability of indigenous peat cellulolytic microorganisms

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