Diversity of Cellulase-Producing Filamentous Fungi From Tibet and Transcriptomic Analysis of a Superior Cellulase Producer Trichoderma harzianum LZ117

Li, Jiaxiang; Zhang, Fei; Jiang, Dan-Dan; Li, Jun; Wang, Feng-Lou; Zhang, Zhang; Wang, Wei; Zhao, Xin‐Qing

doi:10.3389/fmicb.2020.01617

Cited by 25 publications

(15 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although T. harzianum strains have shown high cellulolytic activity (Horta et al, 2018;Li et al, 2020b), most studies investigating this species have focused on biological control (Maruyama et al, 2020;Yan and Khan, 2021). Thus, the regulatory mechanisms underlying hydrolytic enzyme production by these fungi are still poorly explored at the transcriptional level (Delabona et al, 2017(Delabona et al, , 2020(Delabona et al, , 2021.…”

Section: Xyr1 and Cre1 Response To Cellulose Degradationmentioning

confidence: 99%

Network Analysis Reveals Different Cellulose Degradation Strategies acrossTrichoderma harzianumStrains Associated with XYR1 and CRE1

Rosolen¹,

Aono²,

Almeida³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: Trichoderma species have attracted attention as alternative plant polysaccharide-degrading enzyme sources, among other reasons. Most Trichoderma spp., such as Trichoderma atroviride and Trichoderma harzianum, are described as mycoparasitic fungi and are widely used in agriculture as biocontrol agents. T. harzianum is also a potential candidate for hydrolytic enzyme production in which gene expression is tightly controlled. Thus, a better understanding of transcriptional regulation related to the main activator (XYR1) and repressor (CRE1) of cellulases and hemicellulases is necessary. Herein, we explore the genetic mechanisms of the key regulators of genes encoding plant cell wall-degrading enzymes by inferring a gene coexpression network for T. harzianum (IOC-3844 and CBMAI-0179) and, for comparative purposes, for T. atroviride CBMAI-0020.Results: Phylogenetic analysis indicated that both regulatory proteins are widely distributed among ascomycete fungi and suggested how T. atroviride is differentiated from T. harzianum. The coexpression network analyses separated the transcripts into several groups according to the expression profile during growth in cellulose or glucose.Groups with xyr1 or cre1 were identified, and within them, transcripts encoding carbohydrate-active enzymes (CAZymes), TFs, sugar and ion transporters, and proteins with unknown function were coexpressed. However, qualitative and quantitative differences among groups and strains were observed. Core transcripts from these groups (hubs) were identified, and they included transcripts not yet characterized or described as related to cellulose degradation. Several metabolic pathways triggered were recognized with high similarity between both regulators during cellulose degradation, but they differed according to the strains. An enrichment related to mycoparasitism in T. atroviride, especially that associated with CRE1, was observed. It was also noticed that the transcripts encoding CAZymes related to polysaccharide degradation in T.harzianum are not necessarily coexpressed with the TFs studied herein. Conclusion: Our results suggest that different strategies related to XYR1 and CRE1 are used by Trichoderma spp. during cellulose degradation and provide new insights into transcripts coexpressed with both TFs in T. harzianum. This knowledge can be exploited to improve the understanding of the genetic mechanisms involved in hydrolytic enzyme regulation, expanding the potential of T. harzianum use in several industrial applications. 3 Due to the expanding population and industrialization, the demand for energy is increasing throughout the world. Currently, the primary energy source is fossil fuels, i.e., nonrenewable sources such as natural gas, oil, and coal [1]. To reduce the dependency on these finite resources, the development of new sustainable alternatives has become critical. In this context, increasing attention has been given to the development of biofuels from biomass [2, 3]. The United States and Brazil are the world's largest bioet...

show abstract

Section: Xyr1 and Cre1 Response To Cellulose Degradationmentioning

confidence: 99%

Network Analysis Reveals Different Cellulose Degradation Strategies acrossTrichoderma harzianumStrains Associated with XYR1 and CRE1

Rosolen¹,

Aono²,

Almeida³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Although T. harzianum strains have shown high cellulolytic activity ( Horta et al, 2018 ; Li J. X. et al, 2020 ), most studies investigating this species have focused on biological control ( Maruyama et al, 2020 ; Yan and Khan 2021 ). Thus, the regulatory mechanisms underlying hydrolytic enzyme production by these fungi are still poorly explored at the transcriptional level ( Delabona et al, 2017 ; Delabona et al, 2020 ; Delabona et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Network Analysis Reveals Different Cellulose Degradation Strategies Across Trichoderma harzianum Strains Associated With XYR1 and CRE1

et al. 2022

View full text Add to dashboard Cite

Trichoderma harzianum, whose gene expression is tightly controlled by the transcription factors (TFs) XYR1 and CRE1, is a potential candidate for hydrolytic enzyme production. Here, we performed a network analysis of T. harzianum IOC-3844 and T. harzianum CBMAI-0179 to explore how the regulation of these TFs varies between these strains. In addition, we explored the evolutionary relationships of XYR1 and CRE1 protein sequences among Trichoderma spp. The results of the T. harzianum strains were compared with those of Trichoderma atroviride CBMAI-0020, a mycoparasitic species. Although transcripts encoding carbohydrate-active enzymes (CAZymes), TFs, transporters, and proteins with unknown functions were coexpressed with cre1 or xyr1, other proteins indirectly related to cellulose degradation were identified. The enriched GO terms describing the transcripts of these groups differed across all strains, and several metabolic pathways with high similarity between both regulators but strain-specific differences were identified. In addition, the CRE1 and XYR1 subnetworks presented different topology profiles in each strain, likely indicating differences in the influences of these regulators according to the fungi. The hubs of the cre1 and xyr1 groups included transcripts not yet characterized or described as being related to cellulose degradation. The first-neighbor analyses confirmed the results of the profile of the coexpressed transcripts in cre1 and xyr1. The analyses of the shortest paths revealed that CAZymes upregulated under cellulose degradation conditions are most closely related to both regulators, and new targets between such signaling pathways were discovered. Although the evaluated T. harzianum strains are phylogenetically close and their amino acid sequences related to XYR1 and CRE1 are very similar, the set of transcripts related to xyr1 and cre1 differed, suggesting that each T. harzianum strain used a specific regulation strategy for cellulose degradation. More interestingly, our findings may suggest that XYR1 and CRE1 indirectly regulate genes encoding proteins related to cellulose degradation in the evaluated T. harzianum strains. An improved understanding of the basic biology of fungi during the cellulose degradation process can contribute to the use of their enzymes in several biotechnological applications and pave the way for further studies on the differences across strains of the same species.

show abstract

“…Antagonistic fungi play an important role in inhibiting disease development and stimulating the growth of the plant root system (Chandra et al, 2020). Fungi from the genus Trichoderma produce helicase, which destroys the cell walls of pathogens, and other enzymes, in particular, gluconase, chitinase (Abdel-lateif, 2017) and cellulase, which promotes the competitive population of the rhizosphere of plants by this fungus (Cao et al, 2019;Li et al, 2020). Fungi from the genus Trichoderma also limit development of phytopathogens such as Rhizoctonia, Alternaria, Armillaria, Botrytis, Fusarium, Pythium, Phoma, Phytophthora, Ascochyta, Helmintosporium, Colletotrichum and prevent the damage of cereals and vegetable crops caused by root rot (Domsh et al).…”

Section: Resultsmentioning

confidence: 99%

Soybean Productivity in Rice Crop Rotation Depending on the Impact of Biodestructor on Post-Harvest Rice Residues

Dudchenko¹,

Markovska²,

Sydiakina³

2021

Ecol. Eng. Environ. Technol.

View full text Add to dashboard Cite

The use of biodestructors in agricultural technologies for efficient decomposition of crop residues affects the number and species composition of soil fungi, especially pathogenic species, and as a consequence, plant productivity. However, to date, this issue has not been extensively studied. The purpose of this experiment was to develop an effective method of destruction of post-harvest residues using biological products to realize the productive potential of soybeans in rice crop rotation. The work was conducted on the experimental plots of the Institute of Rice NAAS (Skadovsk district, Kherson region, Ukraine) during 2016-2018. In the experiment, the treatment of post-harvest rice residues with a biodestructor Biocomplex-BTU "Ecostern" (1 l/ha) in combination with concentrated amide water-soluble fertilizer, carbamide (30 kg/ha) was carried out in autumn. Application of carbamide alone (30 kg/ha) was used as a control. "Ecostern" is a concentrated agent, which comprises antagonists of pathogenic microorganisms as well as fungi and bacteria that accelerate decomposition of plant residues. The application of biodestructor Biocomplex-BTU "Ecostern" (1 l/ha) in combination with carbamide increased the total number of pathogenic and saprotrophic fungi in the soil from 65.5 to 80.5 thousand /g of soil or by 22.9%. However, the content of pathogenic microflora under this condition was 21.8% lower compared to the control (30 kg/ha carbamide), and the number of saprotrophs increased 3.3-fold. Following the combined use of biodestructor "Ecostern" and carbamide, the number of antagonist fungi has doubled, while the number of toxin-forming fungi decreased by 9.4%. The yield of soybeans also increased by 0.6 t/ha or by 17.9% compared to the control. The increase in yield was observed due to the higher standing density of plants and the number of beans per plant. Before the harvest, the standing density of soybean plants was 45 pcs/m 2 , which is 9.7% higher than the control (41 pcs/m 2 ), due to the high level of field germination of seeds. The number of beans was 24 and 28 pieces per plant, exceeding the control by 16.7%, and the weight of 1000 grains was 156.2 g and 157.5 g, which is 0.8% than the control.

show abstract

Diversity of Cellulase-Producing Filamentous Fungi From Tibet and Transcriptomic Analysis of a Superior Cellulase Producer Trichoderma harzianum LZ117

Cited by 25 publications

References 63 publications

Network Analysis Reveals Different Cellulose Degradation Strategies acrossTrichoderma harzianumStrains Associated with XYR1 and CRE1

Network Analysis Reveals Different Cellulose Degradation Strategies acrossTrichoderma harzianumStrains Associated with XYR1 and CRE1

Network Analysis Reveals Different Cellulose Degradation Strategies Across Trichoderma harzianum Strains Associated With XYR1 and CRE1

Soybean Productivity in Rice Crop Rotation Depending on the Impact of Biodestructor on Post-Harvest Rice Residues

Contact Info

Product

Resources

About