Diversity of bacterial endophyte in Eucalyptus clones and their implications in water stress tolerance

Dasgupta, Modhumita Ghosh; Burragoni, Sravanthi Goud; Amrutha, Sivanantham; Muthupandi, Muthusamy; Parveen, Abdul Bari Muneera; Sivakumar, Veerasamy; Ulaganathan, Kandasamy

doi:10.1016/j.micres.2020.126579

Cited by 11 publications

(13 citation statements)

References 109 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The major phyla in GB stem were Bacteroidetes, Proteobacteria and Actinobacteria while major phyla in PQ stem were Proteobacteria, Bacteroidetes, Acidobacteria and Actinobacteria. A similar finding was reported from a study of Eucalyptus microbiota where Proteobacteria, Bacteroidetes and Firmicutes were the dominant phyla in Eucalyptus leaf 33 . In another study, Proteobacteria was the most abundant phyla in rice phyllosphere followed by Actinobacteria, Bacteroidetes and Firmicutes 34 .…”

Section: Discussionsupporting

confidence: 89%

Niche specificity and functional diversity of the bacterial communities associated with Ginkgo biloba and Panax quinquefolius

Shehata

Ragupathy

Henry

et al. 2021

Sci Rep

View full text Add to dashboard Cite

Plant-associated bacteria can establish mutualistic relationships with plants to support plant health. Plant tissues represent heterogeneous niches with distinct characteristics and may thus host distinct microbial populations. The objectives of this study are to investigate the bacterial communities associated with two medicinally and commercially important plant species; Ginkgo biloba and Panax quinquefolius using high Throughput Sequencing (HTS) of 16S rRNA gene, and to evaluate the extent of heterogeneity in bacterial communities associated with different plant niches. Alpha diversity showed that number of operational taxonomic units (OTUs) varied significantly by tissue type. Beta diversity revealed that the composition of bacterial communities varied between tissue types. In Ginkgo biloba and Panax quinquefolius, 13% and 49% of OTUs, respectively, were ubiquitous in leaf, stem and root. Proteobacteria, Bacteroidetes, Actinobacteria and Acidobacteria were the most abundant phyla in Ginkgo biloba while Proteobacteria, Bacteroidetes, Actinobacteria, Plantomycetes and Acidobacteria were the most abundant phyla in Panax quinquefolius. Functional prediction of these bacterial communities using MicrobiomeAnalyst revealed 5843 and 6251 KEGG orthologs in Ginkgo biloba and Panax quinquefolius, respectively. A number of these KEGG pathways were predicted at significantly different levels between tissues. These findings demonstrate the heterogeneity, niche specificity and functional diversity of plant-associated bacteria.

show abstract

Section: Discussionsupporting

confidence: 89%

Niche specificity and functional diversity of the bacterial communities associated with Ginkgo biloba and Panax quinquefolius

Shehata

Ragupathy

Henry

et al. 2021

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Various microorganisms associated with plants have a significant effect on plant fitness, health and nutrition status. Studies on endophytic bacterial community are most restricted to crop plants, whereas in tree species, such studies are limited to reports from forest ecosystems [ 50 ]. Paulownia sp.…”

Section: Discussionmentioning

confidence: 99%

“…In studies analysing bacteria in the endosphere of a number of plants, Proteobacteria are considered the core dominant phyla, accounting for approximately 50 % of the total bacteria, which is consistent with our results [ 65 , 66 ]. A high relative abundance of this phylum has been reported for different tree species, including apple [ 67 ], ash [ 68 ] and eucalyptus [ 50 ]. The bacterial communities inhabiting the leaves of a given hybrids varied within and across individuals from different localities; however, there was far more variability in the bacterial community structure across the different seasons ( Figure 6 ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Microbial Community, Metabolic Potential and Seasonality of Endosphere Microbiota Associated with Leaves of the Bioenergy Tree Paulownia elongata × fortunei

Woźniak

Gałązka

Marzec-Grządziel

et al. 2022

IJMS

View full text Add to dashboard Cite

The microbial structure and metabolic function of plant-associated endophytes play a key role in the ecology of various environments, including trees. Here, the structure and functional profiles of the endophytic bacterial community, associated with Paulownia elongata × fortunei, in correlation with seasonality, were evaluated using Biolog EcoPlates. Biolog EcoPlates was used to analyse the functional diversity of the microbiome. The total communities of leaf endophyte communities were investigated using 16S rRNA V5–V7 region amplicon deep sequencing via Illumina MiSeq. Community level physiological profiling (CLPP) analysis by the Biolog EcoPlate™ assay revealed that the carboxylic acids (19.67–36.18%) and amino acids (23.95–35.66%) were preferred by all by all communities, whereas amines and amides (0.38–9.46%) were least used. Seasonal differences in substrate use were also found. Based on the sequencing data, mainly phyla Proteobacteria (18.4–97.1%) and Actinobacteria (2.29–78.7%) were identified. A core microbiome could be found in leaf-associated endophytic communities in trees growing in different locations. This work demonstrates the application of Biolog EcoPlates in studies of the functional diversity of microbial communities in a niche other than soil and shows how it can be applied to the functional analyses of endomicrobiomes. This research can contribute to the popularisation of Biolog EcoPlates for the functional analysis of the endomicrobiome. This study confirms that the analysis of the structure and function of the plant endophytic microbiome plays a key role in the health control and the development of management strategies on bioenergy tree plantations.

show abstract

“…The genus Eucalyptus is found in diverse ecological settings and is valued by the bioenergy industry for its short rotation, rapid growth, superior wood properties and high adaptability to different climatic conditions [3,4]. In recent years, some Eucalyptus species have gained market attention as superior sources of lignocellulosic biomass and terpenes, and for their potential in the production of high-power and high-value biofuels [5].…”

Section: Introductionmentioning

confidence: 99%

Clonal propagation of Eucalyptus urophylla under effect of Cyperus rotundus extract and indole-3-acetic acid

et al. 2021

View full text Add to dashboard Cite

Cyperus rotondus tubers possess substances with allelopathic qualities. Some of these substances can act as synergists of plant hormones and be used for root induction in cuttings. This study aimed to shed light on the development and rooting of Eucalyptus urophylla cuttings treated with C. rotondus (nutgrass) tuber extract and IAA (indole-3-acetic acid). The experiments were conducted using a completely randomized design (0; 25; 50; 75 and 100% nutgrass extract and 0; 50; 250; 500; 750 and 1000 mg L-1 of IAA), with four repetitions per treatment and four cuttings per plot. Plantlet height, stem width and root length were measured. The greatest width and height values were recorded for the control (0% nutgrass extract and 0 mg L-1 of IAA), indicating that these substances did not contribute to the development of these variables. However, treatments of nutgrass extract up to 50% were associated with greater root length. C. rotundus extract concentrations of 53% can be used to improve root development in E. urophylla cuttings, and contribute to the production of higher quality plantlets.

show abstract

Diversity of bacterial endophyte in Eucalyptus clones and their implications in water stress tolerance

Cited by 11 publications

References 109 publications

Niche specificity and functional diversity of the bacterial communities associated with Ginkgo biloba and Panax quinquefolius

Niche specificity and functional diversity of the bacterial communities associated with Ginkgo biloba and Panax quinquefolius

Microbial Community, Metabolic Potential and Seasonality of Endosphere Microbiota Associated with Leaves of the Bioenergy Tree Paulownia elongata × fortunei

Clonal propagation of Eucalyptus urophylla under effect of Cyperus rotundus extract and indole-3-acetic acid

Contact Info

Product

Resources

About