Diversity of aquatic Pythium and Phytopythium spp. from rivers and a pond of Gifu city, Japan

Abdelzaher, Hani M.A.; Kageyama, Koji

doi:10.21608/nrmj.2020.130851

Cited by 3 publications

(2 citation statements)

References 13 publications

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“…In Egypt, there is no active control method to reduce damping-off of many crops and vegetable plants caused by P. aphanidermatum although, that pathogenic is widely distributed throughout the country [17,18,21,22]. However, fungicides are regularly used by farmers to manage such diseases without obvious results [6,23]. Pythium aphanidermatum was isolated in this study as the causal agent of G. max damping-off and the pathogenicity test concerned with the pathogenic isolate of P. aphanidermatum was highly virulent to G. max seedlings causing 100% damping off.…”

Section: Discussionmentioning

confidence: 99%

“…The specimens were washed three times with buffer and then postfixed in osmium tetraoxide for 2 h, washed again with buffer, and dehydrated using ascending series of ethanol level (30-100%) for 15 min at each step. The specimens were dried at the critical point and coated with carbon to be examined using the scanning electron microscope (JEOL JSM-6380 LA) operating at 20 kV [30] Pathogenicity and possible control of P. aphanidermatum using P. oligandrum were investigated according to the method described by Abdelzaher and Kageyam [23]. G. max seeds were surface sterilized and germinated to form radicals and plumules for 3 days at 25 • C, then three viable seeds were grown onto 2% WA in a 250 mL Erlenmeyer flask.…”

Section: Identification Of Pythium Speciesmentioning

confidence: 99%

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Myco-Suppression Analysis of Soybean (Glycine max) Damping-Off Caused by Pythium aphanidermatum

Sayed

Abdelmohsen

Abdelzaher

et al. 2021

Plants

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The role of Pythium oligandrum as a biocontrol agent against Pythium aphanidermatum was investigated to avoid the harmful impacts of fungicides. Three isolates of P. oligandrum (MS15, MS19, and MS31) were assessed facing the plant pathogenic P. aphanidermatum the causal agent of Glycine max damping-off. The tested Pythium species were recognized according to their cultural and microscopic characterizations. The identification was confirmed through sequencing of rDNA-ITS regions including the 5.8 S rDNA. The biocontrol agent, P. oligandrum, isolates decreased the mycelial growth of the pathogenic P. aphanidermatum with 71.3%, 67.1%, and 68.7% through mycoparasitism on CMA plates. While the half-strength millipore sterilized filtrates of P. oligandrum isolates degrade the pathogenic mycelial linear growth by 34.1%, 32.5%, and 31.7%, and reduce the mycelial dry weight of the pathogenic P. aphanidermatum by 40.1%, 37.4%, and 36.8%, respectively. Scanning electron microscopy (SEM) of the most effective antagonistic P. oligandrum isolate (MS15) interaction showed coiling, haustorial parts of P. oligandrum to P. aphanidermatum hyphae. Furthermore, P. oligandrum isolates were proven to enhance the germination of Glycine max seedling to 93.3% in damping-off infection using agar pots and promote germination of up to 80% during soil pot assay. On the other hand, P. oligandrum isolates increase the shoot, root lengths, and the number of lateral roots.

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Section: Discussionmentioning

confidence: 99%

Section: Identification Of Pythium Speciesmentioning

confidence: 99%

Myco-Suppression Analysis of Soybean (Glycine max) Damping-Off Caused by Pythium aphanidermatum

Sayed

Abdelmohsen

Abdelzaher

et al. 2021

Plants

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Plant Pathogenic Oomycetes Inhabiting River Water Are a Potential Source of Infestation in Agricultural Areas

Kageyama¹,

Hayano²,

Kikuchi³

et al. 2022

River Basin Environment: Evaluation, Management and Conservation

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New species <i>Pythium amaminum</i> sp. nov. isolated from river water on Amami Island in Japan

Kikuchi,

Hieno,

Suga

et al. 2024

Mycoscience

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Pythium amaminum sp. nov. was isolated from river and reservoir water on Amami island, Kagoshima Prefecture, Japan. The species can grow at temperatures between 10 °C and 35 °C. At the optimum temperature of 25 °C, the radial growth rate is 22.5 mm per day. Pythium amaminum produces filamentous sporangia consisting of branched, lobulate or digitate elements forming large complexes. Zoospores form inside the vesicle, which is discharged through a long tube at least 320 μm. Globose oogonia are ornamented with conical blunt spines. Oospores are aplerotic and globose. Antheridia twine around the oogonia or stick to them. These features having a both of the long discharge tube from sporangium and oogonia with spines are not observed in any other species of the genus Pythium , and thus we conclude that P. amaminum is a new Pythium species.

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Diversity of aquatic Pythium and Phytopythium spp. from rivers and a pond of Gifu city, Japan

Cited by 3 publications

References 13 publications

Myco-Suppression Analysis of Soybean (Glycine max) Damping-Off Caused by Pythium aphanidermatum

Myco-Suppression Analysis of Soybean (Glycine max) Damping-Off Caused by Pythium aphanidermatum

Plant Pathogenic Oomycetes Inhabiting River Water Are a Potential Source of Infestation in Agricultural Areas

New species <i>Pythium amaminum</i> sp. nov. isolated from river water on Amami Island in Japan

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