Diversity of and yeasts (Basidiomycota) inhabiting arbuscular mycorrhizal roots or spores

Abstract

The genera Cryptococcus and Dioszegia contain basidiomycetous yeasts found in a wide range of habitats. Primers to amplify the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA (nrDNA) of arbuscular mycorrhizal fungi (AMF) also allow detecting members of this yeast group. Here we report the results of a sequence analysis using maximum parsimony on a set of 50 ITS sequences of yeasts associated with AMF structures (roots of 26 plant species, AM spores) from six field sites in Central Germany. Among 10 separated taxa, respectively five in the Tremellales and two in the Filobasidiales had unknown sequences. Therefore it was not possible to assign these sequences to any known species. The study indicates that exploring the diversity of Cryptococcus and Dioszegia in soil habitats with molecular methods might enlarge the actually estimated biodiversity of the group.

Fungicide Effects on Fungal Community Composition in the Wheat Phyllosphere

“…aurantiaca . These species have been isolated from both the phyllosphere [43] and the rhizosphere of different plants [44], [45]. Leucosporidium golubevii is a yeast discovered in freshwater [46], and has been reported from the phyllosphere of balsam poplar [47].…”

The isolation and characterization of resident yeasts from the phylloplane of Arabidopsis thaliana

“…Another group of yeast commonly found in the phyllosphere of many plants, Dioszegia spp. have been previously detected on plant leaves and roots, and even polar desert soil10434445. Some novel psychrophilic species in this genus isolated from Antarctica have been previously described45.…”

Dioszegia rishiriensis sp. nov., a novel yeast species from soil collected on Rishiri Island, Hokkaido, Japan

“…Interestingly, most of the species belonging to the genus were isolated from plant leaves and only one of the heretofore described and available species, D. hungarica, the type species, was isolated from soil. The paper of Renker et al (2004) raised the possibility of close associations between plants and Dioszegia species through roots, and isolation of Dioszegia species from soil samples has been reported by Vishniac (2006a) and Connell et al (2008Connell et al ( , 2010.In our course of study of the biodiversity of yeasts in Japan, we isolated two strains belonging to the genus Dioszegia from a soil sample from Rishiri Island in the RishiriRebun-Sarobetsu National Park. After sequence analyses of the D1/D2 domain of the large-subunit (LSU) rRNA gene and internal transcribed spacer (ITS) region, these yeasts were found to represent a new species, phylogenetically closely related to Dioszegia buhagiarii and D. hungarica in the Tremellales, Tremellomycetes, Basidiomycota.…”

“…Interestingly, most of the species belonging to the genus were isolated from plant leaves and only one of the heretofore described and available species, D. hungarica, the type species, was isolated from soil. The paper of Renker et al (2004) raised the possibility of close associations between plants and Dioszegia species through roots, and isolation of Dioszegia species from soil samples has been reported by Vishniac (2006a) and Connell et al (2008Connell et al ( , 2010.…”