Diversity of Aeromonas sp. in Flemish drinking water production plants as determined by gas‐liquid chromatographic analysis of cellular fatty acid methyl esters (FAMEs)

Huys, Geert; Kersters, Llse; Vancanneyt, Marc; Coopman, Renata; Janssen, Paul; Kersters, Karel

doi:10.1111/j.1365-2672.1995.tb03432.x

Cited by 37 publications

(19 citation statements)

References 52 publications

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“…Presumptive Aeromonas isolates were further identified to the genomic species level by using gas-liquid chromatographic analysis of cellular fatty acid methyl esters (FAMEs) as described previously (14). Unknown FAME profiles were compared to the laboratory-based identification library AER48C (13). Isolates that remained unidentified or yielded unreliable FAME identifications were further subjected to whole-genome fingerprinting by using amplified fragment length polymorphism (AFLP) analysis according to the method of Huys and Swings (15).…”

Section: Isolationmentioning

confidence: 99%

Identification and Characterization of Pathogenic Aeromonas veronii Biovar Sobria Associated with Epizootic Ulcerative Syndrome in Fish in Bangladesh

Rahman

Colque-Navarro

Kühn

et al. 2002

Appl Environ Microbiol

156

115

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Sparse information is available on the virulence factors of Aeromonas strains isolated from diseased fish, from the environment, and from humans. In the present study, 52 Aeromonas isolates obtained from epizootic ulcerative syndrome (EUS) lesions in fish, from the aquatic environment, and from children with diarrhea in Bangladesh were identified by biochemical phenotyping (i.e., PhenePlate [PhP] typing) and DNA fingerprinting and then characterized with respect to certain putative virulence factors. The isolates from the fish exhibiting EUS symptoms were identified to be Aeromonas veronii biovar sobria by fatty acid methyl ester analysis and amplified fragment length polymorphism fingerprinting. Biochemical phenotyping revealed that all EUS-associated isolates belonged to a unique phenotype which was not identified among more than 1,600 environmental and diarrheal isolates in a previously collected database of PhP types of Bangladeshi Aeromonas isolates. The 52 Aeromonas isolates were investigated for the production of hemolysin and cytotoxin; for hemagglutination with erythrocytes from fish, human, and rabbit sources; for the presence of a cytolytic enterotoxin gene; and for adhesion to and invasion into fish cell lines. All of the EUS isolates produced all of the virulence factors investigated, as did also some of the environmental isolates, but the isolates from EUS were unique in their ability to agglutinate fish erythrocytes. Our results suggest that a clonal group of A. veronii biovar sobria is associated with, and may be a causative agent of, EUS in fish in Bangladesh.

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Section: Isolationmentioning

confidence: 99%

Identification and Characterization of Pathogenic Aeromonas veronii Biovar Sobria Associated with Epizootic Ulcerative Syndrome in Fish in Bangladesh

Rahman

Colque-Navarro

Kühn

et al. 2002

Appl Environ Microbiol

156

115

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“…Further characterization was carried out by gas-liquid chromatographic analysis of cellular fatty acid methyl esters using the Microbial Identification System (Microbial ID Inc.) as previously described (22). The resulting fatty acid methyl ester patterns were identified automatically and compared with the database AER48C (20) for the identification of mesophilic aeromonads. Isolates that could not be unambiguously classified into one of the known Aeromonas DNA hybridization groups (HGs) or genomic species by comparison with the AER48C database were subjected to whole-genome analysis with the fluorescent amplified fragment length polymorphism analysis technique as described previously (21).…”

mentioning

confidence: 99%

Distribution of Oxytetracycline Resistance Plasmids between Aeromonads in Hospital and Aquaculture Environments: Implication of Tn 1721 in Dissemination of the Tetracycline Resistance Determinant Tet A

Rhodes

Huys

Swings

et al. 2000

Appl Environ Microbiol

349

262

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Oxytetracycline-resistant (OT r ) mesophilic aeromonads were recovered from untreated hospital effluent (72 isolates) and from fish farm hatchery tanks (91 isolates) at sites within the English Lake District, Cumbria, England. The transfer of OT r plasmids from these isolates was investigated. Using Escherichia coli J53-1 as a recipient, 11 isolates from the hospital site and 6 isolates from the fish farm site transferred OT r plasmids (designated pFBAOT1 to 17). Original isolates were identified using fatty acid methyl ester and fluorescent amplified fragment length polymorphism comparisons as either Aeromonas hydrophila HG3 (eight isolates), A. veronii b.v. sobria HG8 (six isolates), and A. caviae HGB5 (one isolate). One isolate remained unidentified, and one could not be assigned a taxonomic designation beyond the genus level. Plasmids pFBAOT1 to -17 were screened for the presence of the tetracycline resistance determinants Tet A to E and Tet G. Only determinant Tet A (10 plasmids) was detected in these plasmids, with 7 tet gene determinants remaining unclassified. In all cases, Tet A was located on a 5.5-kb EcoRI restriction fragment. Hybridization with inc-rep probes N, P, Q, W, and U showed pFBAOT3, -4, -5, -6, -7, -9, and -11, from the hospital environment, to be IncU plasmids. Further, restriction fragment length polymorphism (RFLP) analyses and DNA probing demonstrated that pFBAOT plasmids were closely related to IncU OT r plasmids pASOT, pASOT2, pASOT3, pRAS1 (originally isolated from A. salmonicida strains from fish farms in Scotland and Norway, respectively), and pIE420 (isolated from a German hospital E. coli strain). In addition, DNA analyses demonstrated that plasmids pRAS1 and pIE420 had identical RFLP profiles and that all fragments hybridized to each other. The presence of tetracycline resistance transposon Tn1721 in its entirety or in a truncated form in these plasmids was demonstrated. These results provided direct evidence that related tetracycline resistance-encoding plasmids have disseminated between different Aeromonas species and E. coli and between the human and aquaculture environments in distinct geographical locations. Collectively, these findings provide evidence to support the hypothesis that the aquaculture and human compartments of the environment behave as a single interactive compartment.

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“…Im Grundwasser lassen sich A. hydrophila in sehr geringen Konzentrationen (35 KbE/100 ml) nachweisen [20,21].…”

Section: Vorkommen Im Wasserunclassified

“…B. von Kraftwerken lassen die Aeromonadenzahl (A. hydrophila) in Seen stark ansteigen, bei Temperaturen über 45°C sind sie nicht mehr nachweisbar [23,24]. In Oberflächengewässer dominiert A. hydrophila, gefolgt von A. caviae und A. sobria [21]. Der Eutrophierungsgrad korreliert mit der Anzahl der mesophilen Aeromonaden [25,26].…”

Section: Vorkommen Im Wasserunclassified

Die Bedeutung der beweglichen Aeromonaden als Krankheitserreger für den Menschen

Pund

Theegarten

2008

Bundesgesundheitsbl.

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The genus Aeromonas belongs to the family Aeromonadaceae of the order Aeromonadales and consists of 14 phenospecies and 17 genomospecies. They are gram-negative, oxidase-positive, facultatively anaerobic and glucose-fermenting rods. Members of the mesophilic genus Aeromonas spp. are ubiquitous in the aquatic environment and can be isolated from fresh, brackish and marine water. In nutrient-enriched waters they can attain large populations, particularly in the warm seasons at higher temperatures. Sewage effluents are also a major allochthonous source of the mesophilic aeromonads in the aquatic environment. Organisms of the mesophilic aeromonads also occur in fresh and processed foods, often in very high numbers. They have been isolated from a wide range of both animal and plant food, including raw red meat, fish, seafood or vegetables. This reflects undoubtedly the contact of that food with contaminated water. Species of Aeromonas (A. hydrophila, A. caviae, A. veronii) have been shown to be associated with food borne gastroenteritis, with wound infections acquired via water or with sepsis, peritonitis or meningitis. Unlike gastroenteritis, these infections can have fatal or serious debilitating outcomes. Aeromonas sepsis generally arises secondary to gastroenteritis or wound infection and is associated with high mortality rates. Underlying diseases, particularly liver cirrhosis, or immunsuppressive states also play a major role in the acquisition and outcome of these infections.

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Diversity of Aeromonas sp. in Flemish drinking water production plants as determined by gas‐liquid chromatographic analysis of cellular fatty acid methyl esters (FAMEs)

Cited by 37 publications

References 52 publications

Identification and Characterization of Pathogenic Aeromonas veronii Biovar Sobria Associated with Epizootic Ulcerative Syndrome in Fish in Bangladesh

Identification and Characterization of Pathogenic Aeromonas veronii Biovar Sobria Associated with Epizootic Ulcerative Syndrome in Fish in Bangladesh

Distribution of Oxytetracycline Resistance Plasmids between Aeromonads in Hospital and Aquaculture Environments: Implication of Tn 1721 in Dissemination of the Tetracycline Resistance Determinant Tet A

Die Bedeutung der beweglichen Aeromonaden als Krankheitserreger für den Menschen

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