Identification and Characterization of Pathogenic
            <i>Aeromonas veronii</i>
            Biovar Sobria Associated with Epizootic Ulcerative Syndrome in Fish in Bangladesh

Rahman, Mahbubur; Colque-Navarro, Patricia; Kühn, Inger; Huys, Geert; Swings, Jean; Möllby, R.

doi:10.1128/aem.68.2.650-655.2002

Cited by 156 publications

(128 citation statements)

References 33 publications

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“…Thus, ASP could be an important virulence factor. As it has been reported that motile Aeromonas spp.-associated flurunculosis or ulcerative syndrome is mainly confined to freshwater but not seawater fish (19,26), our present findings probably provide an indication of a low infection rate in seawater fish due to this group of organisms.…”

Section: Discussionmentioning

confidence: 42%

Salt in Surroundings Influences the Production of Serine Protease into Milieu by Aeromonas sobria

Khan

Takahashi

Ramamurthy³

et al. 2007

Microbiology and Immunology

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Previously we have shown that the open reading frame 2 protein (ORF2 protein), which is encoded at the 3′ end of serine protease of Aeromonas sobria (ASP), functions as a chaperone protein in periplasm in the production of ASP. Both proteins, ASP and ORF2 protein, associate in periplasm and ORF2 protein helps ASP to take an active form. ASP which is dissociated from ORF2 protein emerges in milieu. In this study, we examined the effect of sodium chloride (NaCl) in medium on ASP production by A. sobria. The ASP activity of culture supernatant was extremely decreased when A. sobria was cultured in medium containing 3.0% NaCl (concentration almost equivalent to sea water salinity). Our analysis showed that the transcription of asp by A. sobria is not inhibited by NaCl in medium and that A. sobria synthesizes and releases ASP in milieu even under the condition of 3.0% NaCl. However, these ASPs in milieu formed complex as with ORF2 proteins. This indicates that the maturation pathway of ASP is disturbed in A. sobria cultured in medium containing 3.0% NaCl. It is likely that ASP does not associate with ORF2 protein in the correct form in periplasam when A. sobria is cultured in medium containing 3.0% NaCl, though both proteins, ASP and ORF2 protein, make complexes and emerge outside of the cell. This idea suggests that the chaperone system of ASP possesses the ability to sense NaCl in surroundings and regulates the production of active ASP.

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Section: Discussionmentioning

confidence: 42%

Salt in Surroundings Influences the Production of Serine Protease into Milieu by Aeromonas sobria

Khan

Takahashi

Ramamurthy³

et al. 2007

Microbiology and Immunology

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“…It was considered as a significant economic problem, particularly in China and India over the past decade (Citarasu et al 2011). In parts of south-east Asia such as in Bangladesh and India, it was reported that epizootic ulcerative syndrome (EUS) caused by A. sobria resulted in great damage to fish farms (Rahman et al 2002). Intensification of fish farming in Bangladesh has increased the number of disease outbreaks in intensive production systems.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and antibiotic susceptibility of Aeromonas hydrophila isolated from freshwater fishes

Sarder¹,

Khan²,

Saha³

et al. 2016

J. Fish.

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CitationSarder H, Khan T, Saha ML, Punom NJ, Mandal SC and Rahman MS (2016) Prevalence and antibiotic susceptibility of Aeromonas hydrophila isolated from freshwater fishes. Journal of Fisheries 4(3): 411-419. DOI: 10.17017/jfish.v4i3.2016.177 Abstract Aeromonas hydrophila is an opportunistic microorganism. It is a secondary biological agent that contributes to the occurrence of fish diseases and its deterioration. This research was undertaken to determine the prevalence of A. hydrophila in some freshwater fishes collected from three different fish markets of Dhaka City and to test their antibiotic susceptibility. Total bacterial count and total aeromonas on different aeromonas selective media were enumerated using serial dilution technique. Bacterial isolates were characterized to identify A. hydrophila using biochemical tests and with comparison to reference strain (ATCC 7966). The lowest Aeromonas count was detected to be 2.83±0.40×10 2 cfu/g in Anabas testudineus and the highest was 1.03±0.153×10 3 cfu/g in Oreochromis mossambicus. On market basis highest aeromonas count was found in Anando Bazar (8.10±1.09×10 2 cfu/g) and lowest in Hatirpool Bazar (5.63±0.90×10 2 cfu/g) with no significant difference. Maximum susceptibility to amikacin and gentamicin was observed whereas all of the isolates were found resistant to a commonly used antibiotic amoxycillin. The obtained results point that antimicrobial susceptibility was more or less similar regardless of the origin of the samples collected. All the fishes investigated in this study contained A. hydrophila in their different organs.

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“…These bacteria have a broad host range, and often have been implicated in the cause of numerous infections, such as humans with diarrhea and fish with hemorrhagic septicemia (6,20).…”

Section: Introductionmentioning

confidence: 99%

Characterization of pathogenic Aeromonas veronii bv. veronii associated with ulcerative syndrome from Chinese longsnout catfish (Leiocassis longirostris Günther)

Cai¹,

Wu²,

Jian³

et al. 2012

Braz. J. Microbiol.

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ABSTRACT273 bacterial strains were isolated from 20 Chinese longsnout catfish samples. The biochemical characteristics of all strains conformed to the species description of Aeromonas veronii bv. veronii on the basis of Vitek GNI+ card. Furthermore, 16S rDNA, gyrB and rpoD sequences of the representative strain PY50 were sequenced and showed high similarity with A. veronii bv. veronii in Genbank. Antibioticresistance of the representative strain PY50 was assessed by the Kirby-Bauer disk diffusion method, and the results showed it was susceptible and moderately susceptible to 13 and 4 of the 21 antimicrobial agents tested. Extracellular products of strain PY50 contained gelatinase, lecithinase, elastase, most of lipase and lipopolysaccharide. Virulence of strain PY50 and extracellular products to Chinese longsnout catfish were also tested, and LD 50 were about 3.47×10 4 CFU per fish and 11.22 g per fish in intraperitoneal injection respectively. This is the first report that A. veronii bv. veronii was the pathogenic agent of ulcerative syndrome in Chinese longsnout catfish.

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Identification and Characterization of Pathogenic Aeromonas veronii Biovar Sobria Associated with Epizootic Ulcerative Syndrome in Fish in Bangladesh

Cited by 156 publications

References 33 publications

Salt in Surroundings Influences the Production of Serine Protease into Milieu by Aeromonas sobria

Salt in Surroundings Influences the Production of Serine Protease into Milieu by Aeromonas sobria

Prevalence and antibiotic susceptibility of Aeromonas hydrophila isolated from freshwater fishes

Characterization of pathogenic Aeromonas veronii bv. veronii associated with ulcerative syndrome from Chinese longsnout catfish (Leiocassis longirostris Günther)

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