Diversity in the Incorporation into Tissue Phospholipids and Effects on Eicosanoid Production oftrans-Monoene Fatty Acid in Rats Fed with Different Dietary Proteins

Linoleic and α-linolenic acids differently modify the effects of elaidic acid on polyunsaturated fatty acid metabolism and some immune indices in rats

“…In contrast, the compositions of PUFA in liver phospholipids were not essentially modified by the difference in the geometry of octadecenoic acids, and they apparently reflected those of dietary PUFA. It has been reported that trans fatty acids interfere with the desaturation of linoleic acid to arachidonic acid, and hence eicosanoid production (Kinsella et al 1981;Sugano et al 1989), but this effect disappears when the supply of essential linoleic acid is satisfactory (Zevenbergen & Haddeman, 1989;Koga et al 1994). In the present study, the perilla oil diet supplied approximately 2 % energy as linoleic acid, which corresponds to the minimal requirement of this essential fatty acid for rats.…”

“…In the present study, the perilla oil diet supplied approximately 2 % energy as linoleic acid, which corresponds to the minimal requirement of this essential fatty acid for rats. Although a similar response pattern of the PUFA composition of spleen phospholipids is deduced between the cis and trans groups (Koga et al 1994), this dietary level of linoleic acid may be border line in its ability to compensate for the effect of elaidic acid, since splenic production of prostaglandin decreased significantly in rats fed on trans fat in combination with safflowerseed oil. The observation that splenic leukotriene C4 production was not influenced by the dietary fat sources indicates a possible difference in the sensitivity of the enzymic systems producing individual eicosanoids.…”

“…Liver and serum lipids were analysed for cholesterol, triacylglycerol and phospholipid as described elsewhere (Sugano et al 1984). Liver phospholipids were separated into phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine and cardiolipin by TLC, and their fatty acid compositions were analysed as methyl esters by GLC (Koga et al 1994). The adipose tissue lipids were also extracted and their fatty acid compositions analy sed.…”

“…Leukotriene C4 was extracted from the medium and measured by radioimmunoassay using a commercial kit (NEK-030, New England Nuclear, Boston, MA, USA) under the linear relationship with respect to the homogenate volume and incubation time (Gu et al 1994). Splenic prostaglandin E2 production was similarly measured (NEK 020, New England Nuclear) (Koga et al 1994). Serum immunoglobulin (Ig) levels were measured by the sandwich ELISA as reported previously (Gu et al 1994).…”

Effect of Dietary Cabbage Fermentation Extract and Young Barley Leaf Powder on Immune Function of Sprague-Dawley Rats.

“…They are biosynthesized from polyunsaturated fatty acid (PUPA) and closely involved as triggers in inflammatory response and type I allergy (5,6). We have previously reported that dietary fats and some kind of fatty acids modulate production and re lease of eicosanoids (7)(8)(9)(10). These results suggest that lipid metabolism in the liver and the proportion of PUFA in immune-related tissues and cells are responsible for the modulation of eicosanoid production.…”