Diversity in Indian and Some Exotic Onion Cultivars as Revealed by Genomic and Mitochondrial Dna

Chaurasia, Akhilesh K.; Adsul, G. G.; Nair, Dhanya N.; Subramaniam, V. R.; Krishna, Bal; Sane, P. V.

doi:10.17660/actahortic.2010.859.24

Cited by 8 publications

(6 citation statements)

References 24 publications

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“…Previous studies of cytoplasm determination in Indian onion varieties and exotic accessions (Chaurasia et al 2010) revealed that all the varieties, except Arka Kirthiman and 'Arka Pitambar', contained N type cytoplasm. Havey (1993) studied Indian onion accessions and reported them mostly as N cytoplasmic except PI288272 which had N+S cytoplasm.…”

Section: Discussionmentioning

confidence: 96%

“…Preliminary reports on use of PCR markers for cytoplasm determination (Chaurasia et al. , Saini et al. , Sheemar and Dhatt ) have been documented.…”

mentioning

confidence: 99%

“…Two hybrids, Arka Kirthiman and Arka Lalima, have been released by IIHR, Bengaluru, but somehow they did not gain attention of the farmers and were not released at the national level to the farmers. Preliminary reports on use of PCR markers for cytoplasm determination (Chaurasia et al 2010, Saini et al 2015, Sheemar and Dhatt 2015 have been documented.…”

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confidence: 99%

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Limitations of PCR‐based molecular markers to identify male‐sterile and maintainer plants from Indian onion (Allium cepa L.) populations

Khar

Saini

2016

Plant Breeding

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The main aim of this study was to validate PCR markers for determining cytoplasm and genotypes at the Ms locus in short-day onion. Three cytoplasmic (OSN, MKFR and accD) and four nuclear (OPT, jnurf13, AcSKP1 and AcPMS1) markers were employed. Sel. 121-1 had 100% S cytoplasm, whereas Sel. 121-2, 'Pusa Red' and 'Pusa Madhavi' had 88%, 33% and 17% S cytoplasm, respectively. 'Early Grano' and 'Pusa Riddhi' did not possess S cytoplasm. Observations in 33 commercial varieties revealed two with sterile (S) cytoplasm. Nuclear markers were not found in linkage disequilibrium with the Ms locus, and the constitution of Ms alleles by OPT was different from other three markers, which were in conformity with each other. The other three markers predicted that most of the plants should be homozygous recessive. Anther colour also did not confirm the sterility status. It can be concluded that accD may be used for cytoplasm determination based on the ease of its use. For the Ms locus tagging, more markers are needed to be evaluated to isolate maintainer lines from open-pollinated populations.

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Section: Discussionmentioning

confidence: 96%

“…Preliminary reports on use of PCR markers for cytoplasm determination (Chaurasia et al. , Saini et al. , Sheemar and Dhatt ) have been documented.…”

mentioning

confidence: 99%

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Limitations of PCR‐based molecular markers to identify male‐sterile and maintainer plants from Indian onion (Allium cepa L.) populations

Khar

Saini

2016

Plant Breeding

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“…Until now, population studies in onion have been performed using dominant molecular markers, particularly RAPD (Bradeen and Havey, 1995; Kutty et al , 2006; Leite and Anthonisen, 2009), but not ISSR (reviewed by Reddy et al , 2013) markers. Indeed, the latter were used in a study conducted on Indian landraces (Chaurasia and Adsul, 2009). In the present study, we also used ISSR markers and found them to be sufficiently informative and reasonably cost- and labour-effective.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversification of local onion populations under different production systems in Uruguay

Monteverde

Galván

Speranza

2014

Plant Genet. Resour.

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In Uruguay, onion (Allium cepa L.) germplasm is mainly derived from the genetic material introduced by several waves of European immigrants and subsequently multiplied by household farmers, resulting in a wealth of locally adapted populations. This study examined the genetic diversity in a collection of 27 local onion populations and two cultivars derived from them. A total of 843 onion plants were fingerprinted, and 83 inter-simple sequence repeat polymorphic bands were generated. Analysis of molecular variance showed high diversity within the populations (66% of the total variation). Some short-day populations from different geographical origins were grouped together by the unweighted pair-group method using arithmetic averages, principal coordinate analysis and cluster analysis, while the more extensively sampled long- and intermediate-day populations showed a widespread distribution, with no significant subgrouping among them. This weakly structured gene pool is probably the consequence of seed and bulb exchange between farmers and natural inter-pollination. Nevertheless, a Bayesian analysis allowed the distinction of four genetic backgrounds of alleles in the whole collection, and populations were predominately assigned to each genetic background. In addition, mitochondrial variants determining normal (N) pollen fertility or the sterile S or T types were analysed for the same set of plants using specific primers. Most accessions showed a proportion of male-sterile individuals. Cytoplasm type T was the most represented (26 out of 29 accessions), and cytoplasm type S was found in a low proportion of individuals in seven populations. Uruguayan onion germplasm maintains a low degree of genetic differentiation despite the small cultivated area and intense seed exchange, probably due in part to different market purposes based on the growing cycle.

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“…The RFLP approach was applied to identify the CMS genotypes using the probes for the following mitochondrial genes : atpA, atp6, atp9, cob, cox1, nad3, nad4 and nad6 (Szklarczyk et al 2002) and these markers are located in a chloroplast psbA gene amplicon which can distinguish malefertile (N) and male-sterile (S) cytoplasm in onions (Cho et al 2006). Genomic and mitochondrial genome diversity was evaluated by employing RAPD, SSR and RFLP markers (Chaurasia et al 2010).…”

Section: Marker For Cytoplasmic Male Sterility (Cms) In Onionmentioning

confidence: 99%

Molecular markers in the improvement of Allium crops

Chinnappareddy¹,

Khandagale²,

Chennareddy³

et al. 2013

Czech J. Genet. Plant Breed.

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Chinnappareddy L.R.D., Khandagale K., Chennareddy A., Ramappa V.G. (2013) The genus Allium (Family: Alliaceae) is the most important among the bulbous vegetable crops. Characterization of Alliums based on phenotypic traits is influenced by the environment and leads to biased diversity estimates. Recognizing the potential of DNA markers in plant breeding, researchers have adopted the molecular markers for marker-assisted selection (MAS), quantitative trait loci (QTL) mapping and characterization of different quality traits in Alliums. This review presents details about the use of DNA markers in Alliums for cultivar identification, diversity studies, SSR development, colour improvement, total soluble solids (TSS), cytoplasmic male sterility (CMS) and efforts of DNA sequencing. As there are no such reports to describe the above work under a single heading, we decided to mine literature for those who are working in onion, garlic, chives and leek improvement to generate new insights in the subject.

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Diversity in Indian and Some Exotic Onion Cultivars as Revealed by Genomic and Mitochondrial Dna

Cited by 8 publications

References 24 publications

Limitations of PCR‐based molecular markers to identify male‐sterile and maintainer plants from Indian onion (Allium cepa L.) populations

Limitations of PCR‐based molecular markers to identify male‐sterile and maintainer plants from Indian onion (Allium cepa L.) populations

Genetic diversification of local onion populations under different production systems in Uruguay

Molecular markers in the improvement of Allium crops

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