Diversity and structure of the microbial community in rhizosphere soil of Fritillaria ussuriensis at different health levels

Abstract

Fritillaria wilt is a kind of soil-borne disease that causes a large reduction in the yield of Fritillaria ussuriensis. The diversity and structure of the soil microbial community are important factors affecting the health of Fritillaria ussuriensis. The analysis of the microbial community in the diseased and healthy soils provided a theoretical basis for revealing the pathological mechanism and prevention of Fritillaria wilt disease. In the present study, we sequenced the soil microorganisms from healthy (H), pathology (P) and blank (B) soil samples by Illumina MiSeq. Determined the soil physicochemical properties respectively, analyzed the soil microbial diversity and structure, and constructed single factor co-correlation networks among microbial genera. The results showed that Ascomycota (48.36%), Mortierellomycota (23.06%), Basidiomycota (19.00%), Proteobacteria (31.74%), and Acidobacteria (20.95%) were dominant in the soil. The diversity of healthy soil was significantly greater than that of diseased soil samples (P and B) (P < 0.05). The populations of Fusarium and Humicola significantly increased in the diseased soil sample (P and B) (P < 0.05). RB41 (4.74%) and Arthrobacter (3.30%) were the most abundant genera in the healthy soil. Total nitrogen (TN), available nitrogen (AN), total potassium (TK), available potassium (AK), and inorganic salt (salt) were significantly correlated with soil microbial communities (P < 0.05). The relationship between fungi and the plant was mostly positive, whereas bacteria showed the opposite trend. In conclusion, the diversity and structure of the soil microbial community were closely related to the health level of Fritillaria ussuriensis. Fusarium and Humicola affect the severity of Fritillaria wilt disease, while RB41 and Arthrobacter are the important indicators for maintaining the health of Fritillaria ussuriensis. Moreover, environmental factors greatly affect the abundance and formation of soil microbial community. The interactions in microbial communities also influence the healthy growth of Fritillaria ussuriensis.

Structural characteristics and diversity of the rhizosphere bacterial communities of wild Fritillaria przewalskii Maxim. in the northeastern Tibetan Plateau

“…In the current study, the bacterial taxonomic compositions of F. przewalskii from different sites exhibited significant differences. Proteobacteria, Acidobacteria, Bacteroidetes, and Actinobacteria phyla formed the dominant microbiota despite habitat fluctuation (Figure 5A), which is consistent with previous studies (Shi et al, 2011;Jiao et al, 2022). Cyanobacteria was only the dominant phylum at RG site.…”

“…The habitat is extremely vulnerable to natural influences, and human activities including over-harvesting, which have now resulted in F. przewalskii being declared endangered (Chen et al, 2013;Jin et al, 2019). Numerous studies indicated that knowing more about rhizosphere microbiota patterns may enhance the effectiveness of measure designed to assist the adaptation of F. przewalskii to the environment, and resist stress (Mahoney et al, 2017;Jiao et al, 2022). In particular, rhizosphere microbes can improve tolerance of Fritillaria to common diseases, such as wilt and root rot diseases, which can reduce crop yields (Baayen et al, 2001;Song et al, 2016).…”

Research progress of rhizosphere microorganisms in Fritillaria L. medicinal plants

“…The abundance and diversity of soil microbial community decreased in the field evaluation of root rot disease in Fritillaria ussuriensis , while the population of pathogens in the healthy soil sample was quite low, indicating that the microbial community structure affected the health of F. ussuriensis ( Song et al, 2016 ). The diversity and structure of the microbial community were closely related to the health level of F. ussuriensis , this can lay a foundation for the systematic study on the interaction between microbial genera in the pathological process of F. ussuriensis ( Jiao et al, 2022 ).…”

Technology dissemination of Beauveria bassiana bioinsecticide and Trichoderma biofungicide for controlling pests and diseases on shallots

“…Aplikasi pestisida yang tinggi oleh petani ditunjukkan oleh tingginya kadar residu insektisida khususnya chlorpyrifos pada umbi bawang merah yaitu sebesar 0.229 mg/kg atau melebihi batas maksimum yang bisa ditoleransi menurut ISO 1313 (Jamaluddin et al, 2015). Selain memiliki dampak negatif terhadap musuh alami hama dan tanaman (fitotoksik), insektisida juga dapat menurunkan kesuburan tanah (Supreeth et al, 2016) dan sangat berbahaya bagi kesehatan manusia bahkan dalam konsentrasi yang rendah (Arisaputri & Bali, 2016;Jiao et al, 2022;Srinivasulu & Ortiz, 2017).…”