Diversity and phylogeny of rhizobia associated with Desmodium spp. in Panxi, Sichuan, China

Xu, Kai; Zou, Lan; Penttinen, Petri; Zeng, Xiangzhong; Liu, Ming; Zhao, Ke; Chen, Cuiping; Chen, Yuan Xue; Zhu, Xiaoping

doi:10.1016/j.syapm.2015.10.005

Cited by 19 publications

(18 citation statements)

References 34 publications

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“…The Desmodium nodulating rhizobium strains in Panxi region were quite different from those in other places such as temperate and subtropical region of China and Central and North America, possibly due to the special environmental conditions (Xu et al, 2016 ). The faba bean rhizobia in this area were approximately as diverse as in Sichuan hilly areas and in Yunnan (Table 4 ) (Xu et al, 2015 ; Xiong et al, 2017 ), yet more diverse than in other parts of subtropical China (Tian et al, 2007 ; Xiong et al, 2017 ).…”

Section: Discussionmentioning

confidence: 95%

Faba Bean (Vicia faba L.) Nodulating Rhizobia in Panxi, China, Are Diverse at Species, Plant Growth Promoting Ability, and Symbiosis Related Gene Levels

et al. 2018

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We isolated 65 rhizobial strains from faba bean (Vicia faba L.) from Panxi, China, studied their plant growth promoting ability with nitrogen free hydroponics, genetic diversity with clustered analysis of combined ARDRA and IGS-RFLP, and phylogeny by sequence analyses of 16S rRNA gene, three housekeeping genes and symbiosis related genes. Eleven strains improved the plant shoot dry mass significantly comparing to that of not inoculated plants. According to the clustered analysis of combined ARDRA and IGS-RFLP the isolates were genetically diverse. Forty-one of 65 isolates represented Rhizobium anhuiense, and the others belonged to R. fabae, Rhizobium vallis, Rhizobium sophorae, Agrobacterium radiobacter, and four species related to Rhizobium and Agrobacterium. The isolates carried four and five genotypes of nifH and nodC, respectively, in six different nifH-nodC combinations. When looking at the species-nifH-nodC combinations it is noteworthy that all but two of the six R. anhuiense isolates were different. Our results suggested that faba bean rhizobia in Panxi are diverse at species, plant growth promoting ability and symbiosis related gene levels.

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Section: Discussionmentioning

confidence: 95%

Faba Bean (Vicia faba L.) Nodulating Rhizobia in Panxi, China, Are Diverse at Species, Plant Growth Promoting Ability, and Symbiosis Related Gene Levels

et al. 2018

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“…Subsequently, Granada et al [20] studied the genetic diversity of D. incanum-nodulating isolates collected in Brazil. Recently, Xu et al [56] analyzed 34 strains of rhizobia obtained from the root nodules of four wild Desmodium species found in China, and reported the presence of Rhizobium, Pararhizobium, Mesorhizobium and Bradyrhizobium isolates with a high level of diversity. In this current study, the phylogenetic analysis of D. incanum rhizobia symbionts revealed the majority were Bradyrhizobium and isolates clustered with previously characterized Desmodium-nodulating rhizobia according to data available from other countries.…”

Section: Discussionmentioning

confidence: 99%

Nitrogen-fixing rhizobial strains isolated from Desmodium incanum DC in Argentina: Phylogeny, biodiversity and symbiotic ability

Toniutti

Fornasero

Albicoro

et al. 2017

Systematic and Applied Microbiology

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Desmodium spp. are leguminous plants belonging to the tribe Desmodieae of the subfamily Papilionoideae. They are widely distributed in temperated and subtropical regions and are used as forage plants, for biological control, and in traditional folk medicine. The genus includes pioneer species that resist the xerothermic environment and grow in arid, barren sites. Desmodium species that form nitrogen-fixing symbiosis with rhizobia play an important role in sustainable agriculture. In Argentina, 23 native species of this genus have been found, including Desmodium incanum. In this study, a total of 64 D. incanum-nodulating rhizobia were obtained from root nodules of four Argentinean plant populations. Rhizobia showed different abiotic-stress tolerances and a remarkable genetic diversity using PCR fingerprinting, with more than 30 different amplification profiles. None of the isolates were found at more than one site, thus indicating a high level of rhizobial diversity associated with D. incanum in Argentinean soils. In selected isolates, 16S rDNA sequencing and whole-cell extract MALDI TOF analysis revealed the presence of isolates related to Bradyrhizobium elkanii, Bradyrhizobium japonicum, Bradyrhizobium yuanmingense, Bradyrhizobium liaoningense, Bradyrhizobium denitrificans and Rhizobium tropici species. In addition, the nodC gene studied in the selected isolates showed different allelic variants. Isolates were phenotypically characterized by assaying their growth under different abiotic stresses. Some of the local isolates were remarkably tolerant to high temperatures, extreme pH and salinity, which are all stressors commonly found in Argentinean soils. One of the isolates showed high tolerance to temperature and extreme pH, and produced higher aerial plant dry weights compared to other inoculated treatments. These results indicated that local isolates could be efficiently used for D. incanum inoculation.

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“…(Desmodieae) growing in Panxi, Sichuan, China, nodC sequences for one Pararhizobium , one Rhizobium / Agrobacterium , and two Rhizobium isolates characterized on 16S rRNA and housekeeping gene sequences were identical to those for Ensifer strains isolated from L. leucocephala in China (Panxi) and Brazil [ 96 ]. It was concluded that horizontal transfer of nodC genes had occurred between the different genera, but that further work was required to determine the direction of gene transfer [ 96 ]. Evidence was also found for lateral transfer of symbiosis genes between rhizobial genera associated with G. soja (‘wild soybean’).…”

Section: Lateral Transfer Of Symbiosis Genesmentioning

confidence: 99%

Horizontal Transfer of Symbiosis Genes within and Between Rhizobial Genera: Occurrence and Importance

Andrews

Meyer

James

et al. 2018

Genes

102

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Rhizobial symbiosis genes are often carried on symbiotic islands or plasmids that can be transferred (horizontal transfer) between different bacterial species. Symbiosis genes involved in horizontal transfer have different phylogenies with respect to the core genome of their ‘host’. Here, the literature on legume–rhizobium symbioses in field soils was reviewed, and cases of phylogenetic incongruence between rhizobium core and symbiosis genes were collated. The occurrence and importance of horizontal transfer of rhizobial symbiosis genes within and between bacterial genera were assessed. Horizontal transfer of symbiosis genes between rhizobial strains is of common occurrence, is widespread geographically, is not restricted to specific rhizobial genera, and occurs within and between rhizobial genera. The transfer of symbiosis genes to bacteria adapted to local soil conditions can allow these bacteria to become rhizobial symbionts of previously incompatible legumes growing in these soils. This, in turn, will have consequences for the growth, life history, and biogeography of the legume species involved, which provides a critical ecological link connecting the horizontal transfer of symbiosis genes between rhizobial bacteria in the soil to the above-ground floral biodiversity and vegetation community structure.

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Diversity and phylogeny of rhizobia associated with Desmodium spp. in Panxi, Sichuan, China

Cited by 19 publications

References 34 publications

Faba Bean (Vicia faba L.) Nodulating Rhizobia in Panxi, China, Are Diverse at Species, Plant Growth Promoting Ability, and Symbiosis Related Gene Levels

Faba Bean (Vicia faba L.) Nodulating Rhizobia in Panxi, China, Are Diverse at Species, Plant Growth Promoting Ability, and Symbiosis Related Gene Levels

Nitrogen-fixing rhizobial strains isolated from Desmodium incanum DC in Argentina: Phylogeny, biodiversity and symbiotic ability

Horizontal Transfer of Symbiosis Genes within and Between Rhizobial Genera: Occurrence and Importance

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