Faba Bean (Vicia faba L.) Nodulating Rhizobia in Panxi, China, Are Diverse at Species, Plant Growth Promoting Ability, and Symbiosis Related Gene Levels

Chen, Yuan X; Zou, Lan; Penttinen, Petri; Chen, Qiang; Li, Qi Q; Wang, Chang Q; Xu, Kai

doi:10.3389/fmicb.2018.01338

Cited by 25 publications

(19 citation statements)

References 46 publications

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“…Noteworthy, strains closely related to R. hidalgonense have also been isolated from other legumes including Acacia gummifera 78 , Indigofera arrecta in Ethiopia 3 , Trifolium spp. in Ethiopia 79 , T. semipilosum in Kenya 80 , Vicia faba in Ethiopia and China 78 , 81 . The concatenated analysis of recA - atpD showed that all these strains formed a highly bootstrapped cluster with R. hidalgonense FH14 T and displayed high nucleotide identities of recA - atpD (> 99.4%).…”

Section: Resultsmentioning

confidence: 99%

Genetic characterization at the species and symbiovar level of indigenous rhizobial isolates nodulating Phaseolus vulgaris in Greece

Efstathiadou

Ntatsi

Savvas

et al. 2021

Sci Rep

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Phaseolus vulgaris (L.), commonly known as bean or common bean, is considered a promiscuous legume host since it forms nodules with diverse rhizobial species and symbiovars. Most of the common bean nodulating rhizobia are mainly affiliated to the genus Rhizobium, though strains belonging to Ensifer, Pararhizobium, Mesorhizobium, Bradyrhizobium, and Burkholderia have also been reported. This is the first report on the characterization of bean-nodulating rhizobia at the species and symbiovar level in Greece. The goals of this research were to isolate and characterize rhizobia nodulating local common bean genotypes grown in five different edaphoclimatic regions of Greece with no rhizobial inoculation history. The genetic diversity of the rhizobial isolates was assessed by BOX-PCR and the phylogenetic affiliation was assessed by multilocus sequence analysis (MLSA) of housekeeping and symbiosis-related genes. A total of fifty fast-growing rhizobial strains were isolated and representative isolates with distinct BOX-PCR fingerpriniting patterns were subjected to phylogenetic analysis. The strains were closely related to R. anhuiense, R. azibense, R. hidalgonense, R. sophoriradicis, and to a putative new genospecies which is provisionally named as Rhizobium sp. I. Most strains belonged to symbiovar phaseoli carrying the α-, γ-a and γ-b alleles of nodC gene, while some of them belonged to symbiovar gallicum. To the best of our knowledge, it is the first time that strains assigned to R. sophoriradicis and harbored the γ-b allele were found in European soils. All strains were able to re-nodulate their original host, indicating that they are true microsymbionts of common bean.

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Section: Resultsmentioning

confidence: 99%

Genetic characterization at the species and symbiovar level of indigenous rhizobial isolates nodulating Phaseolus vulgaris in Greece

Efstathiadou

Ntatsi

Savvas

et al. 2021

Sci Rep

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“…These efficient bacteria are indicated as plant growth-promoting rhizobacteria (PGPR) [ 12 ]. Seed inoculation with PGPR that can colonize plant roots enhances seed germination rate [ 13 ], root growth and plant development under salt stress as a consequence of its potential in a symbiotic N 2 fixation, and root hydraulic conductivity [ 14 ], production of plant hormones like auxins, cytokinins and gibberellins as well as production of 1-aminocyclopropane-1-carboxylate deaminase [ 15 ], maintaining a high K + /Na + ratio, scavenging reactive oxygen species (ROS), stimulating the activity of soil enzymes [ 16 ], and enhancing soil physicochemical properties [ 17 ]. Consequently, a profitable strategy to mitigate the harmful impacts of salt stress with an increase in plant growth could be the co-inoculation of seeds with different PGPR strains [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Foliar-Applied Potassium Silicate Coupled with Plant Growth-Promoting Rhizobacteria Improves Growth, Physiology, Nutrient Uptake and Productivity of Faba Bean (Vicia faba L.) Irrigated with Saline Water in Salt-Affected Soil

Hafez

Osman

El-Razek

et al. 2021

Plants

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The continuity of traditional planting systems in the last few decades has encountered its most significant challenge in the harsh changes in the global climate, leading to frustration in the plant growth and productivity, especially in the arid and semi-arid regions cultivated with moderate or sensitive crops to abiotic stresses. Faba bean, like most legume crops, is considered a moderately sensitive crop to saline soil and/or saline water. In this connection, a field experiment was conducted during the successive winter seasons 2018/2019 and 2019/2020 in a salt-affected soil to explore the combined effects of plant growth-promoting rhizobacteria (PGPR) and potassium (K) silicate on maintaining the soil quality, performance, and productivity of faba bean plants irrigated with either fresh water or saline water. Our findings indicated that the coupled use of PGPR and K silicate under the saline water irrigation treatment had the capability to reduce the levels of exchangeable sodium percentage (ESP) in the soil and to promote the activity of some soil enzymes (urease and dehydrogenase), which recorded nearly non-significant differences compared with fresh water (control) treatment, leading to reinstating the soil quality. Consequently, under salinity stress, the combined application motivated the faba bean vegetative growth, e.g., root length and nodulation, which reinstated the K+/Na+ ions homeostasis, leading to the lessening or equalizing of the activity level of enzymatic antioxidants (CAT, POD, and SOD) compared with the controls of both saline water and fresh water treatments, respectively. Although the irrigation with saline water significantly increased the osmolytes concentration (free amino acids and proline) in faba bean plants compared with fresh water treatment, application of PGPR or K-silicate notably reduced the osmolyte levels below the control treatment, either under stress or non-stress conditions. On the contrary, the concentrations of soluble assimilates (total soluble proteins and total soluble sugars) recorded pronounced increases under tested treatments, which enriched the plant growth, the nutrients (N, P, and K) uptake and translocation to the sink organs, which lastly improved the yield attributes (number of pods plant−1, number of seeds pod−1, 100-seed weight). It was concluded that the combined application of PGPR and K-silicate is considered a profitable strategy that is able to alleviate the harmful impact of salt stress alongside increasing plant growth and productivity.

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“…It plays a key role in soil fertility restoration through nitrogen fixation by its successful association with Rhizobium sp. (root nodulation bacterium), therefore making a significant contribution to agricultural sustainability [7].…”

Section: Introductionmentioning

confidence: 99%

Mapping of Two New Rust Resistance Genes Uvf-2 and Uvf-3 in Faba Bean

et al. 2021

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Faba bean is gaining attention in Australia as a rotation grain legume where most of the country’s produce is exported. Rust, caused by Uromyces viciae-fabae, is a major constraint to Faba bean production in eastern Australia and its chemical control results in increased cost of production. The deployment of diverse sources of resistance in new cultivars underpins economic and eco-friendly control of rust diseases of crops. A selection from cultivar Doza (Doza#12034) and a European accession Ac1655 exhibited seedling rust resistance against U. viciae-fabae pathotype 24–40. Doza#12034 and Ac1655 were crossed with a susceptible genotype Fiord and recombinant inbred line (RIL) F6 populations were generated. Rust tests on Fiord/Doza#12034 and Fiord/Ac1655 F4 and F6 populations demonstrated monogenic inheritance of resistance in both crosses and the underlying resistance loci were named Uvf-2 and Uvf-3, respectively. Genetic mapping of both RIL populations located Uvf-2 and Uvf-3 in chromosomes III and V, respectively. The SNPs that showed linkage with Uvf-2 and Uvf-3 were converted into kompititive allele specific PCR (KASP) assays. Markers KASP_Vf_0703 and KASP_C250539 flanked Uvf-2 at 4.9 cM and 2.9 cM distances, whereas Uvf-3 was flanked by KASP_Ac×F165 (2.5 cM) and KASP_vf_1090 (10.1 cM). Markers KASP_Vf_0703 and KASP_Ac×F165 can be used for marker-assisted selection of Uvf-2 and Uvf-3, respectively, after confirming parental polymorphisms.

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Faba Bean (Vicia faba L.) Nodulating Rhizobia in Panxi, China, Are Diverse at Species, Plant Growth Promoting Ability, and Symbiosis Related Gene Levels

Cited by 25 publications

References 46 publications

Genetic characterization at the species and symbiovar level of indigenous rhizobial isolates nodulating Phaseolus vulgaris in Greece

Genetic characterization at the species and symbiovar level of indigenous rhizobial isolates nodulating Phaseolus vulgaris in Greece

Foliar-Applied Potassium Silicate Coupled with Plant Growth-Promoting Rhizobacteria Improves Growth, Physiology, Nutrient Uptake and Productivity of Faba Bean (Vicia faba L.) Irrigated with Saline Water in Salt-Affected Soil

Mapping of Two New Rust Resistance Genes Uvf-2 and Uvf-3 in Faba Bean

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