Diversity and phylogenetic analysis of endosymbiotic bacteria from field caught Bemisia tabaci from different locations of North India based on 16S rDNA library screening

Singh, Shalini; Priya, Natarajan Gayatri; Kumar, Arvind; Rana, Vipin Singh; Ellango, R.; Joshi, Adita; Priyadarshini, G. Indira; Asokan, R.; Rajagopal, Raman

doi:10.1016/j.meegid.2012.01.015

Cited by 61 publications

(43 citation statements)

References 39 publications

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“…What seems to be the same bacterium has also been found from whiteflies in India. Recently, Singh et al (30) reported that of the 14 whitefly populations belonging to the Asia I and Asia II genetic groups of B. tabaci from different locations in north India, OLO was present in 7. Apart from whiteflies, bacteria belonging to the same genus as OLO have so far been reported only in genera of the family Aphididae (19,31).…”

Section: Discussionmentioning

confidence: 99%

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Characterization of a Newly Discovered Symbiont of the Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae)

Bing

Yang

Zchori-Fein

et al. 2013

Appl Environ Microbiol

123

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bBemisia tabaci (Hemiptera: Aleyrodidae) is a species complex containing >28 cryptic species, some of which are important crop pests worldwide. Like many other sap-sucking insects, whiteflies harbor an obligatory symbiont, "Candidatus Portiera aleyrodidarum," and a number of secondary symbionts. So far, six genera of secondary symbionts have been identified in B. tabaci. In this study, we report and describe the finding of an additional bacterium in the indigenous B. tabaci cryptic species China 1 (formerly known as B. tabaci biotype ZHJ3). Phylogenetic analysis based on the 16S rRNA and gltA genes showed that the bacterium belongs to the Alphaproteobacteria subdivision of the Proteobacteria and has a close relationship with human pathogens of the genus Orientia. Consequently, we temporarily named it Orientia-like organism (OLO). OLO was found in six of eight wild populations of B. tabaci China 1, with the infection rate ranging from 46.2% to 76.8%. Fluorescence in situ hybridization (FISH) of B. tabaci China 1 in nymphs and adults revealed that OLOs are confined to the bacteriome and co-occur with "Ca. Portiera aleyrodidarum." The vertical transmission of OLO was demonstrated by detection of OLO at the anterior pole end of the oocytes through FISH. Quantitative PCR analysis of population dynamics suggested a complex interaction between "Ca. Portiera aleyrodidarum" and OLO. Based on these results, we propose "Candidatus Hemipteriphilus asiaticus" for the classification of this symbiont from B. tabaci.

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Section: Discussionmentioning

confidence: 99%

“…Hemipteriphilus asiaticus" and the aphid symbiont SMLS (98.8% on average) places them within the genus "Candidatus Hemipteriphilus." So far, "Candidatus Hemipteriphilus" has been detected only from Asian populations of Hemiptera (15,19,30,31).…”

Section: Discussionmentioning

confidence: 99%

Characterization of a Newly Discovered Symbiont of the Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae)

Bing

Yang

Zchori-Fein

et al. 2013

Appl Environ Microbiol

123

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“…Besides the obligatory bacterial endosymbiont Portiera aleyrodidarum [39] Rickettsia , Hamiltonella , Wolbachia , Arsenophonus , Cardinium and Fritschea are described for B. tabaci [32], [40], [41] which can occur singly or in mixed communities with others [32], [42]–[44]. Recently, additional Enterobacter , Bacillus , Paracoccus, Acinetobacter and an Orientia- like organism were found sporadically in whiteflies [45], [46]. Aside from detection and identification of those secondary endosymbionts, there is only little information on their additional biological functions provided to B. tabaci.…”

Section: Discussionmentioning

confidence: 99%

Quantification and Localization of Watermelon Chlorotic Stunt Virus and Tomato Yellow Leaf Curl Virus (Geminiviridae) in Populations of Bemisia tabaci (Hemiptera, Aleyrodidae) with Differential Virus Transmission Characteristics

2014

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Bemisia tabaci (Gennadius) is one of the economically most damaging insects to crops in tropical and subtropical regions. Severe damage is caused by feeding and more seriously by transmitting viruses. Those of the genus begomovirus (Geminiviridae) cause the most significant crop diseases and are transmitted by B. tabaci in a persistent circulative mode, a process which is largely unknown. To analyze the translocation and to identify critical determinants for transmission, two populations of B. tabaci MEAM1 were compared for transmitting Watermelon chlorotic stunt virus (WmCSV) and Tomato yellow leaf curl virus (TYLCV). Insect populations were chosen because of their high and respectively low virus transmission efficiency to compare uptake and translocation of virus through insects. Both populations harbored Rickettsia, Hamiltonella and Wolbachia in comparable ratios indicating that endosymbionts might not contribute to the different transmission rates. Quantification by qPCR revealed that WmCSV uptake and virus concentrations in midguts and primary salivary glands were generally higher than TYLCV due to higher virus contents of the source plants. Both viruses accumulated higher in insects from the efficiently compared to the poorly transmitting population. In the latter, virus translocation into the hemolymph was delayed and virus passage was impeded with limited numbers of viruses translocated. FISH analysis confirmed these results with similar virus distribution found in excised organs of both populations. No virus accumulation was found in the midgut lumen of the poor transmitter because of a restrained virus translocation. Results suggest that the poorly transmitting population comprised insects that lacked transmission competence. Those were selected to develop a population that lacks virus transmission. Investigations with insects lacking transmission showed that virus concentrations in midguts were reduced and only negligible virus amounts were found at the primary salivary glands indicating for a missing or modified receptor responsible for virus attachment or translocation.

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“…Group Ctrl_Jun harbored more Bacteroidetes Lutimonas, a strictly aerobic geuns (Yang et al, 2007), the Deltaproteobacteria Desulfobulbus, another sulfating reducer (Castro et al, 2000), and one of the most popular clade, the Roseobacteria of Alphaproteobacteria. All the indicator genera related to Gammaproteobacteria were from Ctrl_Sep&Nov group, such as the methylotrophic Methylophaga (Janvier and Grimont, 1995); an agricultural insect pest (whitefly) associated Portiera group (Singh et al, 2012;Cass et al, 2014), and the widespread and potentially pathogenic Vibrio (Lee et al, 2002). Other Gammaproteobacteria included the members related to the common…”

Section: Response Of Microbial Communities To Introducing Benthos Andmentioning

confidence: 99%

Response of microbial communities to bioturbation by artificially introducing macrobenthos to mudflat sediments for in situ bioremediation in a typical semi-enclosed bay, southeast China

et al. 2015

Marine Pollution Bulletin

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Diversity and phylogenetic analysis of endosymbiotic bacteria from field caught Bemisia tabaci from different locations of North India based on 16S rDNA library screening

Cited by 61 publications

References 39 publications

Characterization of a Newly Discovered Symbiont of the Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae)

Characterization of a Newly Discovered Symbiont of the Whitefly Bemisia tabaci (Hemiptera: Aleyrodidae)

Quantification and Localization of Watermelon Chlorotic Stunt Virus and Tomato Yellow Leaf Curl Virus (Geminiviridae) in Populations of Bemisia tabaci (Hemiptera, Aleyrodidae) with Differential Virus Transmission Characteristics

Response of microbial communities to bioturbation by artificially introducing macrobenthos to mudflat sediments for in situ bioremediation in a typical semi-enclosed bay, southeast China

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