Diversity and health status specific fluctuations of intrauterine microbial communities in postpartum dairy cows

Wagener, Kenneth B.; Prunner, I.; Pothmann, Harald; Drillich, M.; Ehling‐Schulz, Monika

doi:10.1016/j.vetmic.2014.11.017

Cited by 57 publications

(49 citation statements)

References 39 publications

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“…Unexpectedly, a high number of isolates was observed in cows with VD0, although it was still lower in VD0 and VD1 compared with VD2 and VD3. This finding is in agreement with Wagener et al [10] who detected E. coli and T. pyogenes in uterine samples from cows VD0 and VD1 less frequently than in cows with VD2 and VD3. Another finding was that no bacteria could be isolated from half of the tested cows.…”

Section: Discussionsupporting

confidence: 93%

“…it takes less work per sample [39]. Although FTIR spectroscopy has been used to identify uterine bacteria [7, 10], to the authors’ knowledge, this is the first study evaluating the use of FTIR spectroscopy together with traditional bacteriological identification methods performed on uterine samples from dairy cows. Further studies are required to confirm these findings with a larger number of samples.…”

Section: Discussionmentioning

confidence: 99%

“…At the Institute of Microbiology, the samples were re-suspended and cultured on blood agar plates at 37 °C for 48–72 h. A subsample from each colony was cultured at 37 °C for 48 h. Identification of bacterial isolates by FTIR spectroscopy was performed as previously described [10, 29]. In brief, the isolates were cultivated as cell lawns on tryptic soy agar (Oxoid, TSA, Hampshire, UK) at 30 °C for 24 h. One loop (1 mm diameter) of cell material was suspended in 100 µL sterile deionized water by vortexing.…”

Section: Methodsmentioning

confidence: 99%

“…The FTIR spectral library used to identify the uterine bacteria was comprised in total over 700 bacterial species [30] that included more than 200 records of bacteria isolated and identified in detail from recent samples obtained from Austrian and German cows with uterine infections [7, 10, 31]. …”

Section: Methodsmentioning

confidence: 99%

“…Other frequently isolated pathogens are Prevotella species, Fusobacterium necrophorum , and Fusobacterium nucleatum [8, 9]. Recent studies revealed that the post-partum uterine bacterial community is comprised of more than 200 bacterial species [10]. However, for many of these species pathogenicity and interactions between species have yet to be described.…”

Section: Introductionmentioning

confidence: 99%

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Identification of Escherichia coli and Trueperella pyogenes isolated from the uterus of dairy cows using routine bacteriological testing and Fourier transform infrared spectroscopy

Jaureguiberry

Madoz

Giuliodori³

et al. 2016

Acta Vet Scand

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BackgroundUterine disorders are common postpartum diseases in dairy cows. In practice, uterine treatment is often based on systemic or locally applied antimicrobials with no previous identification of pathogens. Accurate on-farm diagnostics are not available, and routine testing is time-consuming and cost intensive. An accurate method that could simplify the identification of uterine pathogenic bacteria and improve pathogen-specific treatments could be an important advance to practitioners. The objective of the present study was to evaluate whether a database built with uterine bacteria from European dairy cows could be used to identify bacteria from Argentinean cows by Fourier transformed infrared (FTIR) spectroscopy. Uterine samples from 64 multiparous dairy cows with different types of vaginal discharge (VD) were collected between 5 and 60 days postpartum, analyzed by routine bacteriological testing methods and then re-evaluated by FTIR spectroscopy (n = 27).ResultsFTIR spectroscopy identified Escherichia coli in 12 out of 14 samples and Trueperella pyogenes in 8 out of 10 samples. The agreement between the two methods was good with a Kappa coefficient of 0.73. In addition, the likelihood for bacterial growth of common uterine pathogens such as E. coli and T. pyogenes tended to increase with VD score. The odds for a positive result to E. coli or T. pyogenes was 1.88 times higher in cows with fetid VD than in herdmates with clear normal VD.ConclusionsWe conclude that the presence of E. coli and T. pyogenes in uterine samples from Argentinean dairy cows can be detected with FTIR with the use of a database built with uterine bacteria from European dairy cows. Future studies are needed to determine if FTIR can be used as an alternative to routine bacteriological testing methods.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification of Escherichia coli and Trueperella pyogenes isolated from the uterus of dairy cows using routine bacteriological testing and Fourier transform infrared spectroscopy

Jaureguiberry

Madoz

Giuliodori³

et al. 2016

Acta Vet Scand

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Characterization of microbes associated with cervico-vaginal adhesion in the reproductive system of camels (Camelus dromedaries)

Ghoneim

Al-Ahmad

Fayez

et al. 2021

Trop Anim Health Prod

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The Metritis Complex in Cattle

Sheldon¹,

Sheldon²

2019

Veterinary Reproduction and Obstetrics

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Retained fetal membranesRetained fetal membranes (RFM) are defined as the failure of an animal to expel the fetal membranes, within 24 hours of the end of parturition. Retained placenta is an alternative name used for RFM. There is some variation in the literature about the duration of retention that defines the clinical disorder. Some prefer to define retention as being for12 hours, but the timing is arbitrary and most normal cows expel the fetal membranes within a few hours of parturition. The incidence of RFM varies amongst herds, but is typically 5 to 10% of animals.The importance of RFM is that they are associated with reduced milk yield and an increased risk of metritis. MetritisMetritis is most common within 10 days of parturition. Metritis is characterized by an enlarged uterus and a watery red-brown fluid, to viscous off-white purulent, uterine discharge, which often has a fetid odour (Sheldon et al 2006). The incidence of metritis varies between breed, country and herd, but in a study of the records from 97,318 cows in the USA, the lactation incidence of metritis, including RFM, was 21% (Zwald et al 2004). However, in some studies the incidence is as high as 40% of the herd. The associated clinical signs are used to classify the severity of disease, which varies from unapparent disease to fatal toxaemic metritis. Clinical endometritisClinical endometritis is defined as the presence of a purulent uterine discharge detectable in the vagina of cattle 21 days or more postpartum, or a mucopurulent discharge detectable in the vagina after 26 days postpartum (Sheldon et al 2006). The incidence of clinical endometritis is around 10 to 20%, with variation between breed, country and herd; a typical study reported that 16.9% of 1,865 dairy cows were affected in Canada (LeBlanc et al

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Diversity and health status specific fluctuations of intrauterine microbial communities in postpartum dairy cows

Cited by 57 publications

References 39 publications

Identification of Escherichia coli and Trueperella pyogenes isolated from the uterus of dairy cows using routine bacteriological testing and Fourier transform infrared spectroscopy

Identification of Escherichia coli and Trueperella pyogenes isolated from the uterus of dairy cows using routine bacteriological testing and Fourier transform infrared spectroscopy

Characterization of microbes associated with cervico-vaginal adhesion in the reproductive system of camels (Camelus dromedaries)

The Metritis Complex in Cattle

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