Diversity and genetic differentiation inArtemia species and populations detected by AFLP markers

Sun, Yuena; Song, Wenqin; Zhong, Yiren; Zhang, Runsheng; Abatzopoulos, Theodore J.; Chen, Ruiyang

doi:10.1007/bf02442119

Cited by 27 publications

(23 citation statements)

References 12 publications

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“…On the contrary, RFLP results indicate a close relationship between A. urmiana and parthenogenetic Artemia which has been confirmed by many other studies using different approaches (BEARDMORE and ABREU-GROBOIS, 1983;ABREU-GROBOIS and BEARDMORE, 1991;ABATZOPOULOS et al, 1997;TRIANTAPHYLLIDIS et al, 1997a;SUN et al, 1999b;BOSSIER et al, 2004;BAXEVANIS et al, 2006;QIU et al, 2006). Therefore, in view of this close genetic relationship between sexual A. urmiana and the Iranian parthenogens and taking into account that Artemia morphometrics are influenced by the environmental conditions, we suggest that within a genus even genetically similar populations may exhibit striking differences in their morphological features.…”

Section: Resultssupporting

confidence: 73%

Morphometric and Preliminary Genetic Characteristics of Artemia Populations from Iran

Agh

Bossier

Abatzopoulos

et al. 2009

International Review of Hydrobiology

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Six Artemia populations, one bisexual and five parthenogenetic, from different parts of Iran were compared using morphometric and genetic characteristics. The discriminant analysis based on 19 morphometric variables showed that there are significant differences between the studied populations based on their morphological characteristics, where 85.9% of original grouped cases were correctly classified. The bisexual Artemia urmiana however exhibited a 100% separation from the parthenogenetic populations. However, a 1500 bp mitochondrial rDNA fragment showed similar RFLP patterns for all Iranian populations confirming earlier reports of a close genetic relationship between A. urmiana and parthenogenetic Artemia. IntroductionThe brine shrimp Artemia (Crustacea, Anostraca) is found abundantly in athalassohaline and thalassohaline environments at salinity levels ranging from 10 g/l (AGH et al., 2007) to 340 g/l (POST and YOUSSEF, 1977). The genus is a complex of bisexual and parthenogenetic species and probably superspecies defined by the criterion of reproductive isolation (BROWNE and BOWEN, 1991;PILLA and BEARDMORE, 1994). All bisexual species are diploid while asexual populations may be diploid, polyploid or a mixture of different ploidies (ABATZOPOU-LOS et al., 2003). Bisexual species are grouped as (1) the New World species: A. franciscana and A. persimilis and (2) the Old World species: A. salina, A. sinica, A. urmiana, A. tibetiana and Artemia sp. Kazakhstan (PILLA and BEARDMORE, 1994;ABATZOPOULOS et al., 2002b). The parthenogenetic forms are found only in the Old World and are grouped, rather controversially, under the binomen A. parthenogenetica (ABATZOPOULOS et al., 2002a, b). Populations of Artemia are found in more than 600 habitats distributed across the world in salt lakes and natural and man-made salterns ( VAN STAPPEN, 2002). Their distribution reflects the flight paths of some migratory birds and deliberate inoculations for commercial purposes by man (PERSOONE and SORGELOOS, 1980). AGH et al. (2002) and ABATZOPOULOS et al. (2006) reported Artemia populations from 17 biotopes in Iran, all parthenogenetic populations except for the bisexual Artemia urmiana. Due to the wide distribution of saline lakes and lagoons in Iran, the presence of Artemia in many geographic locations seems likely. Although the presence of Artemia in Urmia Lake was reported more than 100 years ago (GÜNTHER, 1899; see also ASEM, 2008), Iranian populations remained among the least studied populations so far.It is both due to the economic importance of Artemia and its critical role in larviculture of fish and shellfish that, in recent years, there has been a worldwide effort to discover new Artemia strains with specific responses to environmental conditions and to characterize them with regard to their potential use in aquaculture. Therefore, the characterization of Artemia populations and/or species has been a continuous endeavor since the second half of the previous century .Many scientists consider the genus Artemia as a c...

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Section: Resultssupporting

confidence: 73%

Morphometric and Preliminary Genetic Characteristics of Artemia Populations from Iran

Agh

Bossier

Abatzopoulos

et al. 2009

International Review of Hydrobiology

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“…Despite the use of flow cytometry in the most recent evaluations of crustacean genome size [11]–[13], so far no flow cytometry-based estimates of the Artemia genome have been published. To date, genomic resources for Artemia have been limited to RAPD [14], [15], RFLP [16], AFLP [17], [18], microsatellite markers [19] and the 15,822 bp mitochondrial genome sequence [20], [21].…”

Section: Introductionmentioning

confidence: 99%

A first AFLP-Based Genetic Linkage Map for Brine Shrimp Artemia franciscana and Its Application in Mapping the Sex Locus

et al. 2013

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We report on the construction of sex-specific linkage maps, the identification of sex-linked markers and the genome size estimation for the brine shrimp Artemia franciscana. Overall, from the analysis of 433 AFLP markers segregating in a 112 full-sib family we identified 21 male and 22 female linkage groups (2n = 42), covering 1,041 and 1,313 cM respectively. Fifteen putatively homologous linkage groups, including the sex linkage groups, were identified between the female and male linkage map. Eight sex-linked AFLP marker alleles were inherited from the female parent, supporting the hypothesis of a WZ–ZZ sex-determining system. The haploid Artemia genome size was estimated to 0.93 Gb by flow cytometry. The produced Artemia linkage maps provide the basis for further fine mapping and exploring of the sex-determining region and are a possible marker resource for mapping genomic loci underlying phenotypic differences among Artemia species.

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“…Artemia tibetiana from Lagkor Co (type‐locality) has been characterized by biometrics of cysts and nauplii, cytogenetics, allozymes, randomly amplified polymorphic DNAs (RAPDs), cross‐breeding tests (Abatzopoulos et al. 1998, 2002b), Amplified Fragment Length Polymorphism (AFLP) profiles (Sun et al. 1999b), thermal tolerance of cysts (Clegg et al.…”

Section: Introductionmentioning

confidence: 99%

Evolutionary assessment of Artemia tibetiana (Crustacea, Anostraca) based on morphometry and 16S rRNA RFLP analysis

Baxevanis

Triantaphyllidis²,

Kappas

et al. 2005

J Zoological System

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Over the last few years, molecular-based assays in the genus Artemia have considerably enriched prior systematic assessments based on morphometry. For the first time, morphometric and 16S rRNA PCR-RFLP analyses on all type-locality bisexual species of the genus have been employed here. Emphasis was put on the Asian species (Artemia urmiana, A. tibetiana, A. sinica) where patterns of divergence and reproductive isolation are rather discrete, and especially on A. tibetiana for which recent reports have questioned its specific status. Discriminant analysis of morphometric characters has shown significant differentiation among species. Classification scores were 99.4 and 100% for males and females, respectively. Mitochondrial DNA RFLP patterns have given indications for lower, albeit similar patterns of differentiation compared with those obtained by morphometry. Artemia tibetiana and A. urmiana are mitochondrially indistinguishable which is suggestive of recent ancestry. Our data, in conjunction with past evidence, are supportive of a significant amount of divergence between A. tibetiana and A. sinica. Morphometric and molecular assays can be reciprocally informative provided theory and patterns of speciation are incorporated into systematic assessments.

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Diversity and genetic differentiation inArtemia species and populations detected by AFLP markers

Cited by 27 publications

References 12 publications

Morphometric and Preliminary Genetic Characteristics of Artemia Populations from Iran

Morphometric and Preliminary Genetic Characteristics of Artemia Populations from Iran

A first AFLP-Based Genetic Linkage Map for Brine Shrimp Artemia franciscana and Its Application in Mapping the Sex Locus

Evolutionary assessment of Artemia tibetiana (Crustacea, Anostraca) based on morphometry and 16S rRNA RFLP analysis

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