Diversity and genetic differentiation among subpopulations of Gliricidia sepium revealed by PCR-based assays

Abstract

Randomly amplified polymorphic DNA (RAPD), and a mitochondrial marker based on amplification of the V7 region of the mitochondrial small ribosomal RNA (srRNA) gene, were used to partition genetic variation within a single population of Gliricidia sepium sampled from Guatemala. Seventeen per cent of the variation detected with RAPDs was partitioned among subpopulations and indicated a greater level of discrimination than previously detected with isozymes. Cluster analysis indicated a direct relationship between this variation and the geographical distance between subpopulations. A polymorphism identified within the maternally inherited mitochondrial V7 srRNA product, which relied on digestion with restriction endonucleases, confirmed the genetic subdivision identified with RAPDs, and suggested a relatively limited role for seed in gene dispersal.

Characterization of trees, fruits and genetic diversity in natural populations of mangaba

“…The closer to zero, the smaller is the diversity. This index is considered a good tool for the analysis of populations when using dominant markers (Dawson et al, 1995).…”

Simple Sequence Repeat (SSR) Marker Assay-Based Genetic Diversity among Dolichos Bean (Lablab purpureus L. Sweet) Advanced Breeding Lines Differing for Productivity per se Traits

“…Shanon's diversity index, being relatively insensitive to bias caused by inability to detect heterozygous individuals (Dawson et al, 1995) is being used to measure population diversity (Bussel, 1999). Shanon's diversity index is being largely used as a measure of diversity in plant genetic resources (Upadhayaya et al, 2007).…”

Genetic characterization and evaluation of anthropogenic impacts on genetic patterns in cultured and wild populations of mussels (Mytilus galloprovincialis) from Greece

“…Nevertheless, the proportion of polymorphic loci is not regarded as a good method to describe genetic variation, while more appropriate measures like effective number of alleles and heterozygosity are more efficient estimators (Elmaci et al, 2007;Tiira and Primmer, 2009). Especially for RAPD data, I is considerably suitable since it remains unaffected by the method's inability to distinguish heterozygous individuals but also does not require the presumption that populations are in Hardy-Weinberg equilibrium (Dawson et al, 1995).…”