Diversity and Ecophysiology of New Isolates of Extremely Acidophilic CS ₂ -Converting Acidithiobacillus Strains

Abstract

Biofiltration of industrial carbon disulfide (CS 2 )-contaminated waste air streams results in the acidification of biofilters and therefore reduced performance, high water use, and increased costs. To address these issues, we isolated 16 extremely acidophilic CS 2 -converting Acidithiobacillus thiooxidans strains that tolerated up to 6% (vol/vol) sulfuric acid. The ecophysiological properties of five selected strains (2Bp, Sts 4-3, S1p, G8, and BBW1) were compared. These five strains had pH optima between 1 (2Bp) and 2 (S1p). Their affinities for CS 2 ranged between 80 (G8) and 130 (2Bp) M. Strains S1p, G8, and BBW1 had more hydrophobic cell surfaces and produced less extracellular polymeric substance than did strains 2Bp and Sts 4-3. All five strains converted about 80% of the S added as CS 2 to S 0 when CS 2 was supplied in excess. The rate of S 0 consumption varied between 7 (Sts 4-3) and 63 (S1p) nmol O 2 min ؊1 ml culture ؊1 . Low S 0 consumption rates correlated partly with low levels of cell attachment to externally produced S 0 globules. During chemostat growth, the relative amount of CS 2 hydrolase in the cell increased with decreasing growth rates. This resulted in more S 0 accumulation during CS 2 overloads at low growth rates. Intermittent interruptions of the CS 2 supply affected all five strains. Strains S1p, G8, and BBW1 recovered from 24 h of starvation within 4 h, and strains 2Bp and Sts 4-3 recovered within 24 h after CS 2 was resupplied. We recommend the use of mixtures of Acidithiobacillus strains in industrial biofilters.

Hydrogen and Carbon Monoxide-Utilizing Kyrpidia spormannii Species From Pantelleria Island, Italy

“…In fact, the minor gas species represent the driving forces for the establishment of an active microbial community (Shock et al, 2010;Lindsay et al, 2019). Commonly, geothermal soils are characterized by high temperatures and often a low pH, which may be caused by the presence of H 2 S and CS 2 in the emitted gases and their conversion to sulfuric acid by sulfide-oxidizing microbes (Chiodini et al, 2001;D'Alessandro et al, 2009;Smeulders et al, 2011Smeulders et al, , 2013Quatrini and Johnson, 2018). Despite the harsh conditions, these hot and acidic terrestrial areas harbor distinctive microbial communities (Colman et al, 2019a,b;Lindsay et al, 2019).…”

“…Alphaproteobacterial methanotrophs were isolated from the low NH 4 + site (Gagliano et al, 2014). Previously, key players in the conversion of H 2 S, CS 2 , and CH 4 from the Solfatara geothermal area near Naples, Italy, have been isolated and characterized (Pol et al, 2007;Smeulders et al, 2011Smeulders et al, , 2013van Teeseling et al, 2014). Besides H 2 S, CS 2 and CH 4 , H 2 and CO are available and suitable electron donors for novel chemolithoautotrophs in geothermal soils.…”