Role of Thiobacillus thioparus in the biodegradation of carbon disulfide in a biofilter packed with a recycled organic pelletized material

Abstract

This study reports the biodegradation of carbon disulfide (CS2) in air biofilters packed with a pelletized mixture of composted manure and sawdust. Experiments were carried out in two lab-scale (1.2 L) biofiltration units. Biofilter B was seeded with activated sludge enriched previously on CS2-degrading biomass under batch conditions, while biofilter A was left as a negative inoculation control. This inoculum was characterized by an acidic pH and sulfate accumulation, and contained Achromobacter xylosoxidans as the main putative CS2 biodegrading bacterium. Biofilter operation start-up was unsuccessfully attempted under xerophilic conditions and significant CS2 elimination was only achieved in biofilter A upon the implementation of an intermittent irrigation regime. Sustained removal efficiencies of 90-100 % at an inlet load of up to 12 g CS2 m(-3) h(-1) were reached. The CS2 removal in this biofilter was linked to the presence of the chemolithoautotrophic bacterium Thiobacillus thioparus, known among the relatively small number of species with a reported capacity of growing on CS2 as the sole energy source. DGGE molecular profiles confirmed that this microbe had become dominant in biofilter A while it was not detected in samples from biofilter B. Conventional biofilters packed with inexpensive organic materials are suited for the treatment of low-strength CS2 polluted gases (IL <12 g CS2 m(-3) h(-1)), provided that the development of the adequate microorganisms is favored, either upon enrichment or by inoculation. The importance of applying culture-independent techniques for microbial community analysis as a diagnostic tool in the biofiltration of recalcitrant compounds has been highlighted.

Complete genome sequence of Pseudomonas stutzeri S116 owning bifunctional catalysis provides insights into affecting performance of microbial fuel cells

“…Previously reported SOB belongs to the genera Thiomonas , Acidithiobacillus , Thiothrix , Pseudomonas , Thiobacillus , Halothiobacillus , Chlorobium , Rhodospirillum and Sulfurimonas [ 3 – 5 ]. Currently, studies of SOB have mainly focused on metal recovery from minerals [ 6 ], removal of high concentrations of H 2 S from biogas and industrial waste gas [ 7 , 8 ], and wastewater treatment containing reduced sulfur compounds (S 2− , S 2 O 3 2− , S) [ 9 ]. However, few studies have reported the application of SOB in microbial fuel cells (MFCs) and the related mechanisms have rarely been investigated.…”

Functional biodiversity and plasticity of methanogenic biomass from a full-scale mesophilic anaerobic digester treating nitrogen-rich agricultural wastes

“…rRNA-based PCR amplification to verify their purity (Prenafeta-Boldú et al, 2014). RNA extracts were subsequently transcribed to cDNA by means of PrimeScript™ RT reagent Kit (Perfect Real Time, Takara) following the manufacturer's instructions.…”

Synthesis of Biodegradable Polyisobutylene Disulfides by Living Reversible Recombination Radical Polymerization (R3P): Macrocycles?

“…Polymers containing disulfide (−S–S−) repeat units in the main chain are known as polydisulfide polymers. − Disulfide bonds can be cleaved in a reducing environment to generate thiols (−SH) − and thiols can be transformed into disulfides in an oxidizing atmosphere. This dynamic disulfide/thiol redox system is a unique reversible chemical reaction that can frequently be observed in living organisms and plays a pivotal role in protein folding to generate the active conformation. , The oxidizing extracellular and reducing intracellular environment generates a high redox potential difference .…”