Diversity and bioactive potential of culturable fungal endophytes of Dysosma versipellis; a rare medicinal plant endemic to China

Tan, Xiaoming; Zhou, Yaqin; Zhou, Xiaolei; Xia, Xianghua; Wei, Ying; He, Lili; Tang, Hongzhen; Yu, Liying

doi:10.1038/s41598-018-24313-2

Cited by 63 publications

(42 citation statements)

References 78 publications

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“…The results showed that there was a speci c microbiome in 27 samples of tree medicinal licorices, and the relative abundance of endophytic fungi was correlated with the host plant species and root depth. For example, Ascomycota was the dominant phylum in all samples, followed by Basidiomycota, which result consistent with previous studies [48,49]. The phylum Ascomycota, as the largest phylum of fungi, has diverse populations and plays an important role in genetics [50], ecology [51] and phylogeny [52].…”

Section: Discussionsupporting

confidence: 90%

Root-associated endophytic fungal community composition and structure of three medicinal licorices in Xinjiang

Dang¹,

Wang²,

Li³

et al. 2020

Preprint

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The purpose of this study was to explore the diversity and composition of endophytic fungal community in the root of three medicinal licorices, and learn more about its biological characteristics by analyzing its interaction with soil and root factors. A total of 2,118,633 effective sequences and 1,063 effective operational taxonomic units (OTUs) with 97% identity were obtained by high-throughput sequencing among 27 samples. In this study, a total of 8 phyla and 140 genera were annotated, among them, the phylum Ascomycota and Basidiomycota, and the genera Fusarium, Paraphoma and Helminthosporium were significantly dominant in the 27 samples. Wilcoxon rank sum test showed that the Shannon index was significantly different distribution between Glycyrrhiza uralensis and Glycyrrhiza inflata, especially 0-20cm at the root depth, the Chao1 index in Glycyrrhiza inflate was significantly affected by root depth, and there were significant differences in beta diversity between Glycyrrhiza uralensis and Glycyrrhiza inflata. Distance-based redundancy analysis (db-RDA) showed that soil physicochemical properties (available potassium and ammonium nitrogen), and the root factor (liquiritin and water content) were the main contributing factors to the variations in the overall structure of endophytic fungal community in this study. This study provides useful information for formulating strategies to improve the quantity and quality of medicinal licorices.

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Section: Discussionsupporting

confidence: 90%

Root-associated endophytic fungal community composition and structure of three medicinal licorices in Xinjiang

Dang¹,

Wang²,

Li³

et al. 2020

Preprint

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“…In addition to their interactions with plants, some members of Burkholderia have been shown to have an impact on fungal reproduction, and were suggested to be part of the mycorrhizal intracellular microbiome [9,73]. The genus Cadophora comprises several root endophytes with antimicrobial activity [74,75]. Cadophora species were also found to produce high levels of plant cell wall-degrading enzymes, as well as small, secreted proteins and aquaporins that typically function in tissue colonization and nutrient acquisition in the intercellular spaces of host plants [76,77].…”

Section: Discussionmentioning

confidence: 99%

Experimental Evidence of Microbial Inheritance in Plants and Transmission Routes from Seed to Phyllosphere and Root

Abdelfattah

Wisniewski

Schena

et al. 2020

Preprint

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Background While the environment is considered the primary origin for establishing the microbiome of newly developing plants, the potential role of seeds as a source of transmitting microorganisms has not received much attention. Here we tested the hypothesis that the plant microbiome is at least partially inherited through vertical transmission. We investigated where microbes reside within a seed, and how microbes are subsequently transmitted from the seed to seedling’s belowground and aboveground plant tissues. To this aim, an experimental culturing device was constructed to grow oak seedlings in a microbe-free environment while keeping the belowground and aboveground tissues separated.Results Amplicon sequencing of fungal ITS2 and bacterial 16S rDNA revealed that the seed microbiome is diverse and non-randomly distributed within an acorn. The large majority (> 95%) of fungi and bacteria present in the acorn were transmitted to the seedling. The microbial composition of the phyllosphere strongly resembled the composition found in the embryo, whereas the roots and pericarp each had a distinct microbial community. Similar relationships were observed for both fungi and bacteria.Conclusion Our findings demonstrate a high level of microbial diversity and spatial partitioning of the fungal and bacterial community within both seed and seedling, indicating inheritance, niche differentiation, and divergent transmission routes for the establishment of root and phyllosphere communities in oak seedlings. The study provides new information on the importance of the maternal environment as a source of the seedling microbiome, with significant implications for our understanding of the continuity of the plant microbiome across generations.

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“…Taxol, a widely employed anticancer drug, was originally isolated from the bark of Taxus brevifolia , and have also been produced by a series of endophytic fungi from different plants [ 12 , 13 ]. Tan et al isolated two endophytic fungi producing the podophyllotoxin, a main active compound from the host plant Dysosma versipellis [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Diversity and Antimicrobial Activity of Endophytic Fungi Isolated from Chloranthus japonicus Sieb in Qinling Mountains, China

Shi

et al. 2020

IJMS

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The plant Chloranthus japonicus Sieb is known for its anticancer properties and mainly distributed in China, Japan, and Korea. In this study, we firstly investigated the diversity and antimicrobial activity of the culturable endophytic fungi from C. japonicus. A total of 332 fungal colonies were successfully isolated from 555 tissue segments of the medicinal plant C. japonicus collected from Qinling Mountains, China. One hundred and thirty representative morphotype strains were identified according to ITS rDNA sequence analyses and were grouped into three phyla (Ascomycota, Basidiomycota, Mucoromycota), five classes (Dothideomycetes, Sordariomycetes, Eurotiomycetes, Agaricomycetes, Mucoromycetes), and at least 30 genera. Colletotrichum (RA, 60.54%) was the most abundant genus, followed by Aspergillus (RA, 11.75%) and Diaporthe (RA, 9.34%). The Species Richness Index (S, 56) and the Shannon-Wiener Index (H′, 2.7076) indicated that C. japonicus harbored abundant fungal resources. Thirteen out of 130 endophytic fungal ethyl acetate extracts exhibited inhibitory activities against at least one pathogenic bacterium or fungus. Among of these, F8158, which was identified as Trichoderma cf. harzianum, exhibited good antagonistic capacities (the percent inhibition of mycelial growth ranged from 47.72~88.18) for different pathogens and has a potential application in biological control. In addition, it is noteworthy that the strain F8157 (Thanatephorus cucumeris, an opportunistic pathogen) showed antibacterial and antifungal activity, which is reported firstly in this study, and should be investigated further. Taken together, these results indicated that the endophytic fungi from C. japonicus may be of potential interest in screening bio-control agents and discovering of new bioactive compounds.

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Diversity and bioactive potential of culturable fungal endophytes of Dysosma versipellis; a rare medicinal plant endemic to China

Cited by 63 publications

References 78 publications

Root-associated endophytic fungal community composition and structure of three medicinal licorices in Xinjiang

Root-associated endophytic fungal community composition and structure of three medicinal licorices in Xinjiang

Experimental Evidence of Microbial Inheritance in Plants and Transmission Routes from Seed to Phyllosphere and Root

Diversity and Antimicrobial Activity of Endophytic Fungi Isolated from Chloranthus japonicus Sieb in Qinling Mountains, China

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