Diversity and Antimicrobial Activity of Endophytic Fungi Isolated from Chloranthus japonicus Sieb in Qinling Mountains, China

An, Chao; Ma, Saijian; Shi, Xinwei; Xue, Wenjiao; Liu, Chen; Ding, Hao

doi:10.3390/ijms21175958

Cited by 25 publications

(22 citation statements)

References 43 publications

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“…In our previous study, it was found that there was some similarity between the endophytic fungi composition of C. japonicus Sieb (dominant genera: Colletotrichum, Aspergillus, and Diaporthe ) and that of T. chinensis Baker (dominant genera: Collectotrichum, Fusarium, Aspergillus ) from Yingpan town (Qinling Mountains region), Shaanxi province of China. However, the results obtained in this study (dominant genera: Plectosphaerella, Paraphoma, and Fusarium ) showed significant difference from those of the aforementioned studies ( An et al, 2020a ; An et al, 2020b ). Also, rare genera such as Lecanicillium , Sarocladium , Thelonectria , Valutella , Exserohilum , Flavodon , and Rigidoporus obtained in this study were quite different from those in our previous studies, including Biscogniauxia , Leptostroma , Stachybotrys , Didymella , Peniophora , Peniophora , Rhodotorula , Setophaeosphaeria , Clitopilus , Muyocopron , Thanatephorus , and Ceriporia .…”

Section: Discussioncontrasting

confidence: 97%

Diversity and Ginsenoside Biotransformation Potential of Cultivable Endophytic Fungi Associated With Panax bipinnatifidus var. bipinnatifidus in Qinling Mountains, China

Shi

et al. 2022

Front. Pharmacol.

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To obtain novel fungi with potent β-glucosidase for minor ginsenoside production, Panax bipinnatifidus var. bipinnatifidus, which is a traditional medicinal plant containing various ginsenosides, was first employed to isolate endophytic fungi in this study. A total of 93 representative morphotype strains were isolated and identified according to ITS rDNA sequence analyses, and they were grouped into three phyla (Ascomycota, Basidiomycota, and Mucoromycota), five classes (Dothideomycetes, Sordariomycetes, Eurotiomycetes, Agaricomycetes, and Mucoromycetes), and 24 genera. Plectosphaerella (RA, 19.35%) was the most abundant genus, followed by Paraphoma (RA, 11.83%) and Fusarium (RA, 9.70%). The species richness index (S, 34) and the Shannon–Wiener index (H’, 3.004) indicated that P. bipinnatifidus harbored abundant fungal resources. A total of 26 endophytic fungal ethyl acetate extracts exhibited inhibitory activities against at least one pathogenic bacterium or fungus. In total, 11 strains showed strong β-glucosidase activities and also presented with the ability of ginsenoside biotransformation with varied glycoside-hydrolyzing pathways. Excitingly, three genera, namely, Ilyonectria, Sarocladium, and Lecanicillium, and all 11 taxa were first found to have the ability to transform ginsenosides in our study. The results indicated that P. bipinnatifidus could be a new fungi resource with potential novel natural compounds with antimicrobial activity and potent β-glucosidase for varied minor ginsenoside production.

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Section: Discussioncontrasting

confidence: 97%

Diversity and Ginsenoside Biotransformation Potential of Cultivable Endophytic Fungi Associated With Panax bipinnatifidus var. bipinnatifidus in Qinling Mountains, China

Shi

et al. 2022

Front. Pharmacol.

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“…In contrast to the dominance of Colletotrichum spp. in these medicinal plants (Tan et al, 2018;An et al, 2020), Penicillium followed by Aspergillus were the most dominating fungal taxa in the current study. In another study on fungal endophytes isolated from Rhodiola spp.…”

Section: Microbial Communities In a Euchromamentioning

confidence: 56%

“…For example, Tan et al (2018) reported 93% occurrence of Ascomycota in Dysosma versipellis. An et al (2020) reported endophytic fungal diversity of Chloranthus japonicus, where 97.89% of endophytes belonged to the Ascomycota phylum. In contrast to the dominance of Colletotrichum spp.…”

Section: Microbial Communities In a Euchromamentioning

confidence: 99%

Arnebia euchroma, a Plant Species of Cold Desert in the Himalayas, Harbors Beneficial Cultivable Endophytes in Roots and Leaves

et al. 2021

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The endophytic mutualism of plants with microorganisms often leads to several benefits to its host including plant health and survival under extreme environments. Arnebia euchroma is an endangered medicinal plant that grows naturally in extreme cold and arid environments in the Himalayas. The present study was conducted to decipher the cultivable endophytic diversity associated with the leaf and root tissues of A. euchroma. A total of 60 bacteria and 33 fungi including nine yeasts were isolated and characterized at the molecular level. Among these, Proteobacteria was the most abundant bacterial phylum with the abundance of Gammaproteobacteria (76.67%) and genus Pseudomonas. Ascomycota was the most abundant phylum (72.73%) dominated by class Eurotiales (42.42%) and genus Penicillium among isolated fungal endophytes. Leaf tissues showed a higher richness (Schao1) of both bacterial and fungal communities as compared to root tissues. The abilities of endophytes to display plant growth promotion (PGP) through phosphorus (P) and potassium (K) solubilization and production of ACC deaminase (ACCD), indole acetic acid (IAA), and siderophores were also investigated under in vitro conditions. Of all the endophytes, 21.51% produced ACCD, 89.25% solubilized P, 43.01% solubilized K, 68.82% produced IAA, and 76.34% produced siderophores. Six bacteria and one fungal endophyte displayed all the five PGP traits. The study demonstrated that A. euchroma is a promising source of beneficial endophytes with multiple growth-promoting traits. These endophytes can be used for improving stress tolerance in plants under nutrient-deficient and cold/arid conditions.

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“…Du et al (2020) reported colony growth inhibition of Colletotrichum siamense only by 3.6-26.3% under the influence of endophytic fungi from Securinega suffruticosa roots, stems and leaves. The endophytic fungi isolated from the tissues of Chloranthus japonicus exhibited good antagonistic capacities (the percent inhibition of mycelial growth ranged 47.72-88.18%) against different pathogens (An et al, 2020). Thus, antagonistic ability of the endophytic isolates derived from the peel and pulp of Ch.…”

Section: Discussionmentioning

confidence: 96%

Endophytic community of Chaenomeles speciosa fruits: Screening for biodiversity and antifungal activity

Lykholat

Дідур

Drehval

et al. 2022

Regul. Mech. Biosyst.

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Fruit crops of the genus Chaenomeles Lindl are considered today as a superfood due to accumulation of biologically active compounds with antioxidant ability and known health-promoting properties. Successful introduction of this non-traditional culture in the steppe zone of Ukraine characterised by an unfavourable climate suggests the functioning of effective protective mechanisms in plants, including those that can be provided by the influence of endophytic microorganisms. However, there is little information about the endophytic community of Chaenomeles plants. Herein, the current study was aimed to isolate the endophytic fungi from the Ch. speciosa fruits and evaluate their biological activities against the phytopathogens. The study was carried out based on the collection of the Botanical Garden of Oles Honchar Dnipro National University (Dnipro city, Ukraine). Three media, namely PDA, MPA, and Gause’s medium were used for isolation of endophytic fungi. Colonies of isolates for identification were grown on PDA, Czapek's agar, and Czapek’s yeast autolysate media. Six fungal endophytic isolates derived from both peel and pulp of Ch. speciosa fruits have been morphologically identified using macroscopic and microscopic techniques, and assigned to the genus Penicillium (sections Chrysogena, Penicillium, Viridicata), and genus Talaromyces (section Talaromyces). Species P. expansum, P. viridicatum, and P. hirsutum were identified among the peel isolates, while P. chrysogenum, P. cyclopium, and P. purpurogenum were among the pulp isolates. Antagonistic ability of the endophytic isolates against phytopathogenic fungi was evaluated using the dual culture method. The results showed moderate to high antifungal capacity of the endophytic isolates against the phytopathogenic strains of the Fusarium genus. The growth inhibition of F. culmorum mycelium due to the influence of endophytic isolates was 51.5–81.3%, and the inhibition of the growth of F. oxysporum colonies was in the range of 68.4–86.6% as compared with control. There were no significant differences in the antagonistic ability between endophytic isolates derived from the peel and pulp of the fruit. Taken together, our findings indicated the great potential of the endophytic fungi from Ch. speciosa fruits as a source for the development of biocontrol agents and discovery of new bioactive compounds.

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Diversity and Antimicrobial Activity of Endophytic Fungi Isolated from Chloranthus japonicus Sieb in Qinling Mountains, China

Cited by 25 publications

References 43 publications

Diversity and Ginsenoside Biotransformation Potential of Cultivable Endophytic Fungi Associated With Panax bipinnatifidus var. bipinnatifidus in Qinling Mountains, China

Diversity and Ginsenoside Biotransformation Potential of Cultivable Endophytic Fungi Associated With Panax bipinnatifidus var. bipinnatifidus in Qinling Mountains, China

Arnebia euchroma, a Plant Species of Cold Desert in the Himalayas, Harbors Beneficial Cultivable Endophytes in Roots and Leaves

Endophytic community of Chaenomeles speciosa fruits: Screening for biodiversity and antifungal activity

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