Diversity analysis of some selected rice genotypes through SSR- based molecular markers

Abstract

An experiment was carried out to assess the genetic diversity of advanced rice (Oryza sativa L.) breeding lines using three SSR markers viz., RM147, RM167 and RM215. Thirty T-aman advanced breeding lines at F 9 generation along with 4 check varieties were assessed. All three primers showed polymorphism. A total of 29 alleles were detected among the rice genotypes with an average of 9.67 alleles per locus. Polymorphism information content (PIC) ranged from 0.47 to 0.88 with an average of 0.71. A dendrogram was constructed based on total microsatellite polymorphism and 34 genotypes were grouped into four major clusters at 0.36 similarity co-efficient differentiating the early maturing genotypes from the late maturing types. The information about the genetic diversity might be utilized in future breeding programs for developing rice varieties with much shorter growth duration. The results also suggested that microsatellite markers which are linked to genes or QTLs responsible for growth duration properties are suitable tools for marker assisted selection (MAS) to select the rice genotypes of shorter growth duration.

Characterization of Rice Genotypes Based on Physical, Chemical and Molecular Methods

“…Presence of polymorphism between genotypes revealed that the presence of genetic diversity at the molecular level was high among the selected genotype which implies that, the genetic diversity is more than the morphological diversity and there is ample scope to utilize the material in the breeding programme. These results corroborate the earlier findings of Shahriar et al, (2014), Kundur et al, (2015) and Maruthi (2016) in rice.…”

Genetic variation via SSR polymorphic information content and ecological distribution of Nigerian sesame

“…A moderately high level of diversity was found among the 12 loci examined ranging between 0.38 and 0.79 with average of 0.62. The implication of the number of allele diversity and their frequency among the accessions is referred to as polymorphic information content (PIC) (Shahriar et al, 2014). PIC value of each marker is estimated on the (Table 3).…”

Genetic variability analysis of partially salt tolerant local and inbred rice (Oryza sativa L.) through molecular markers

“…The results obtained from these studies are also comparable to this study. Shahriar et al (2014) reported an average PIC value of 0.71 among 34 Bangladeshi Aman rice using 3 SSR markers. In another study, Siddique et al (2016) found a PIC value of 0.90 among 96 Bangladeshi Aman rice employing 8 SSR markers.…”

“…The results obtained from these studies are also comparable to this study. Shahriar et al. (2014) reported an average PIC value of 0.71 among 34 Bangladeshi Aman rice using 3 SSR markers.…”