Diversity analysis of different Diaporthe (Phomopsis) species and development of molecular marker to identify quarantine important species Phomopsis phaseolorum

Leguminous Seedborne Pathogens: Seed Health and Sustainable Crop Management

“…The genus Diaporthe , together with its anamorph Phomopsis , consists of a huge number of species, and it includes phytopathogenic, saprophytic, and symbiotic endophytic fungi [ 96 , 97 ]. Taxonomically, it has been classified as Eukarya, kingdom Fungi, subkingdom Dikarya, phylum Ascomycota, class Sordariomycetes, order Diaporthales, family Diaporthaceae , and genus Diaporthe [ 41 ].…”

“…In soybean, Diaporthe species are the causal agents of seed decay, seed rot, pod and stem blights, and stem cankers ( Figure 7 ) [ 98 ]. However, pathogens belonging to this genus are able to infect several plant species, producing a variety of symptoms, including damping off, leaf spots, dieback, wilt, and fruit and root rot [ 97 , 100 , 101 ]. Usually, soybean seeds colonized by Diaporthe/Phomopsis are smaller than healthy ones, but they do not show other evident symptoms.…”

“…Usually, soybean seeds colonized by Diaporthe/Phomopsis are smaller than healthy ones, but they do not show other evident symptoms. Otherwise, in some cases, the seeds can be broken and covered with grayish-white mycelium [ 97 , 102 ]. Extensive taxonomic studies have been performed, and initially, the colony morphology, the host of the infection, physiology, and aggressiveness were utilized to identify the components of the Diaporthe genus [ 97 ].…”

Current understanding of the Diaporthe/Phomopsis complex causing soybean stem canker: A focus on molecular aspects of the interaction

“…Alpha‐ and beta‐conidia of Diaporthe spp. can be distinguished by their form and size; alpha‐conidia are one‐celled, biguttulate, ellipsoidal to oval, subcylindrical, fusiform, hyaline and pointed at both ends, while beta‐conidia are single‐celled, aseptate, filiform, curved at one end, eguttate and hyaline (Hosseini et al., 2020; Nishmitha et al., 2022). In D .…”

“…caulivora and D . longicolla , sizes range from 1.3 to 4.0 × 3.7 to 8.5 μm for alpha‐conidia and from 1.3 to 1.9 × 9.3 to 31.6 μm for beta‐conidia (Hosseini et al., 2020; Nishmitha et al., 2022; Rupe, 2015).…”