Diversity analysis of Central Asia and Caucasian lentil (Lens culinaris Medik.) germplasm using SSR fingerprinting

Abstract

Diversity analysis was performed among 39 cultivated lentil (Lens culinaris Medik.) accessions of Central Asia and Caucasian origin using five highly polymorphic microsatellite markers. A total of 33 alleles determined ranging from 3 to 8 per locus. Estimated gene diversity value for 33 loci was 0.66. Genetic similarity indices among 39 accessions ranged from 0.24 to 1.0. Cluster analysis using the unweighted pair group method with arithmetic mean method classified accessions into six major groups at 0.5 similarity coefficient. More than half accessions from Tajikistan formed large cluster. On the other hand, a few accessions from each country showed unique genotypes. Overall, most of the accessions, except ones with closely related origin, were distinguished by the present high quality DNA fingerprinting. This molecular diversity information gives important basis for conservation strategy in gene bank and exotic germplasm introduction in breeding programs in Central Asia and Caucasian countries.

Legume Crops Phylogeny and Genetic Diversity for Science and Breeding

“…The DNA-based markers, such as RFLP (Havey and Muehlbauer, 1989;Muench et al, 1991;Rajora and Mahon, 1994), RAPD (Sharma et al, 1995;Ferguson et al, 2000;Sonnante and Pignone, 2001;Toklu et al, 2009;Tewari et al, 2012), AFLP (Sharma et al, 1996;Zavodna et al, 2000;Duran and Perez de le Vega, 2004;Kahraman et al, 2004;Rubeena et al, 2006;Fiocchetti et al, 2009;Toklu et al, 2009), microsatellite markers (Duran and Perez de le Hamwieh et al, 2009;Liu et al, 2008;Babayeva et al, 2009;Reddy et al, 2010;Gupta et al, 2012, Tewari et al, 2012Zaccardelli et al, 2012), inter simple sequence repeat (ISSR) (Zavodna et al, 2000;Sonnante and Pignone, 2001;Toklu et al, 2009), internal transcribed spacers (ITS) (Fernandez et al, 2000;Mayer and Bagga, 2002;Sonnante et al, 2003), non-transcribed spacer (NTS) (Fernandez et al, 2005), sequenced tagged microsatellite site (STMS) (Inder et al, 2008;Datta et al, 2011), single-nucleotide polymorphisms (SNPs) (Alo et al, 2011), and resistance gene analogue (RGA) (Yaish et al, 2004;Sari et al, 2013) were used to analyze phylogenetic relationships among taxa in the genus Lens, as well as the genetic diversity of cultivated lentil.…”

“…A comparison of 30 landraces from South Asia to 130 from 13 other countries using RAPDs and isozymes was performed by . Numerous small-scale (less than 45 lentil lines) studies have been published using various DNA marker classes: RAPDs (Fikiru et al, 2010;Hoque and Hasan, 2012), ISSRs (Fikiru et al, 2011), AFLPs and SSRs (Sultana and Ghafoor 2008;Babayeva et al, 2009;Reddy et al, 2010;Datta et al, 2011;Zaccardelli et al, 2012). Seed proteins of 13 polymorphic peptides were investigated in 144 lentil landraces collected in Pakistan (Sultana et al, 2006).…”

Genetic Diversity of Cultivated Lentil (Lens culinaris Medik.) and Its Relation to the World's Agro-ecological Zones

“…These include restriction fragment length polymorphisms (RFLPs, Havey and Muehlbauer, 1989), amplified fragment length polymorphisms (AFLPs, Sharma et al, 1996; Alghamdi et al, 2014), random amplified polymorphic DNAs (RAPDs, Abo-Elwafa et al, 1995; Ford et al, 1997; Ferguson et al, 1998; Sonnante and Pignone, 2001), and inter simple sequence repeats (ISSRs, Scippa et al, 2008; Toklu et al, 2009; El-Nahas et al, 2011). Simple sequence repeats (SSRs) have the most widely used DNA markers for assessing genetic diversity in lentil (see Liu et al, 2008; Babayeva et al, 2009; Kaur et al, 2011; Zaccardelli et al, 2012; Dikshit et al, 2015; Idrissi et al, 2015). …”

Characterization of Italian lentil (Lens culinaris Medik.) germplasm by agronomic traits, biochemical and molecular markers

“…Among molecular markers, SSRs (Simple Sequence Repeats) or microsatellites, have been shown to produce polymorphism from a different number of repetitive core motifs present at one locus, and are a valid tool for landrace fingerprinting (Hamwieh et al 2009). Five highly polymorphic SSR markers have been successfully used to fingerprint and assess genetic diversity in a number of cultivated lentil from Central Asia and Caucasian countries (Babayeva et al 2009). A few number of SSRs were able to provide significant insights on genetic diversity in 25 lentil accessions from different areas (Bacchi et al 2010).…”