Diversity among isolates of squash mosaic virus.

Haudenshield, J. S.; Palukaitis, Peter

doi:10.1099/0022-1317-79-10-2331

Cited by 26 publications

(25 citation statements)

References 19 publications

(22 reference statements)

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“…No binding occurred even to virions of the related comoviruses RCMV and CPSMV (for similarities between comoviruses see Haudenshield & Palukaitis, 1998). These results suggest that in vivo only homologous virions can be moved from cell to cell by the viral transport tubule and that the MP is not able to assist the movement of virions even of related viruses.…”

Section: Discussionmentioning

confidence: 72%

The C-terminal region of the movement protein of Cowpea mosaic virus is involved in binding to the large but not to the small coat protein

Carvalho

Wellink²,

Ribeiro

et al. 2003

Journal of General Virology

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Cowpea mosaic virus (CPMV) moves from cell to cell as virus particles which are translocated through a plasmodesmata-penetrating transport tubule made up of viral movement protein (MP) copies. To gain further insight into the roles of the viral MP and capsid proteins (CP) in virus movement, full-length and truncated forms of the MP were expressed in insect cells using the baculovirus expression system. Using ELISA and blot overlay assays, affinity purified MP was shown to bind specifically to intact CPMV virions and to the large CP, but not to the small CP. This binding was not observed with a C-terminal deletion mutant of the MP, although this mutant retained the capacity to bind to other MP molecules and to form tubules. These results suggest that the C-terminal 48 amino acids constitute the virion-binding domain of the MP.

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Section: Discussionmentioning

confidence: 72%

The C-terminal region of the movement protein of Cowpea mosaic virus is involved in binding to the large but not to the small coat protein

Carvalho

Wellink²,

Ribeiro

et al. 2003

Journal of General Virology

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show abstract

“…However, the extensions of this approach to other comoviruses were not particularly revealing, and no obvious discriminating criteria could be derived for subgrouping Andean potato mottle virus (APMV) (46) and several strains of squash mosaic viruses (SqMV) (15,19). The results were especially ambiguous in the case of APMV, which shares a high degree of similarity with BPMV but a comparatively low degree of similarity with CPSMV (15). This discrepancy is inconsistent with the previous placement of BPMV and CPSMV in the same subgroup (9).…”

Section: Fig 2 Averaged Electron Density Of Rcmv (A)mentioning

confidence: 99%

“…It is therefore reasonable to suggest that, as in the case of BPMV, a pentameric annular structure is not formed, supporting the previous subgrouping of CPSMV with BPMV. When the structural alignments are extended to include the sequences of the S proteins of APMV (46) and SqMV (15,19), subgrouping with BPMV is predicted in each case (Fig. 8).…”

Section: Fig 2 Averaged Electron Density Of Rcmv (A)mentioning

confidence: 99%

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Structural Fingerprinting: Subgrouping of Comoviruses by Structural Studies of Red Clover Mottle Virus to 2.4-Å Resolution and Comparisons with Other Comoviruses

Lin

Clark

Chen

et al. 2000

J Virol

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Red clover mottle virus (RCMV) is a member of the comoviruses, a group of picornavirus-like plant viruses. The X-ray structure of RCMV strain S has been determined and refined to 2.4 Å. The overall structure of RCMV is similar to that of two other comoviruses, Cowpea mosaic virus (CPMV) and Bean pod mottle virus (BPMV). The sequence of the coat proteins of RCMV strain O were modeled into the capsid structure of strain S without causing any distortion, confirming the close resemblance between the two strains. By comparing the RCMV structure with that of other comoviruses, a structural fingerprint at the N terminus of the small subunit was identified which allowed subgrouping of comoviruses into CPMV-like and BPMV-like viruses.Red clover mottle virus (RCMV) is a member of the comoviruses, a group of plant viruses in the picornavirus superfamily with nonenveloped, icosahedral capsids and bipartite, singlestranded, positive-sense RNA genomes. As with other comoviruses, the two RNA molecules (termed RNA 1 and RNA 2) are encapsidated separately in isometric particles made up of 60 copies each of a large (L) and a small (S) coat protein (31,34,47). A number of strains of RCMV have been characterized (reference 28 and references therein), and the complete nucleotide sequence of the genome of one of them, RCMV strain S (34), has been determined (41,43,44). Inspection of the RNA sequence together with in vitro translation data (42,45) indicates that RCMV has a mode of gene expression similar to that of the type member of the group, Cowpea mosaic virus (CPMV). Comparison of the RNA 2 sequences of RCMV strain S with those of CPMV, Bean pod mottle virus (BPMV) and Cowpea severe mosaic virus (CPSMV) appears to divide comoviruses into two subgroups, with RCMV and CPMV forming one subgroup and CPSMV and BPMV forming the other (9).One notable feature of RCMV is the ability of different strains of the virus to form viable pseudorecombinants (35). Pseudorecombinants between strains S and O have proved useful in mapping symptom and host range determinants (11,36). The viability of the pseudorecombinants indicates that the coat proteins encoded by the RNA 2 of one strain can be processed correctly by the RNA 1-encoded 24K proteinase of another and are able to efficiently encapsidate the RNA 1 of the heterologous strain. This implies that any differences in the amino acid sequences of the coat proteins from the different strains do not dramatically alter their structure and assembly characteristics.Our basic knowledge of comovirus structure has been derived from crystallographic studies of CPMV and BPMV (10, 31, 48; T. Lin, Z. Chen, R. Usha, C. V. Stauffaacher, J.-B. Dai, T. Schmidt, and J. E. Johnson, submitted for publication). In both cases the structures reveal architectures typical of Pϭ3 plant and animal viruses, with the asymmetric unit consisting of three eight-stranded antiparallel ␤-sandwich domains (38) (Fig. 1). The A domain is located around the fivefold axes and comprises the S subunit of either 24 kDa (CPMV) or 22 kDa ...

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“…However, even with a 32-year span, the analyzed region of the CP genes remained remarkably conserved. This high conservation is also observed for other comoviruses, such as Squash mosaic virus (SQMV), which Sequence variability in the coat protein gene of Cowpea severe mosaic virus... TABLE 1 -Percentage of amino acid (above the diagonal) and nucleotide (below the diagonal) sequence identities between the CP genes of Brazilian CPSMV isolates, the North American isolate DG, and Cowpea mosaic virus (CPMV), the most closely related member from the genus Comovirus showed a nucleotide sequence identity of 96.6% for the CP gene of five isolates from the USA, and Radish mosaic virus (RaMV), with a maximum value of 98.4% of amino acid sequence identity for the CP L (Haudenshield & Palukaitis, 1998;Komatsu et al, 2008).…”

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confidence: 99%

Sequence variability in the coat protein gene of Cowpea severe mosaic virus isolates from northeastern Brazil

Beserra¹,

Andrade²,

Camarço³

et al. 2011

Trop. plant pathol.

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The comovirus Cowpea severe mosaic virus (CPSMV) causes a disease in cowpea (Vigna unguiculata) but, despite its importance, there are no studies on the genetic diversity of viral populations. We have determined the nucleotide sequences of part of the coat protein (CP) gene of six Brazilian isolates of CPSMV. Genomic fragments (521 nucleotides) were RT-PCR amplified, cloned, and their sequences were compared with each other and with other comoviruses. Sequence comparisons indicated a high degree of conservation for the CP gene, with 92-100% nucleotide and 97-100% amino acid sequence identity among the isolates. There was no correlation between geographical origin and sequence identity or phylogeny among the isolates. Key words: Comovirus, Vigna unguiculata, CPSMV, sequence diversity. RESUMO Variabilidade de sequências do gene da proteína do capsídeo de isolados do Cowpea severe mosaic virus do Nordeste do BrasilO comovírus Cowpea severe mosaic virus (CPSMV) causa a doença em feijão-caupi (Vigna unguiculata) conhecida como mosaico severo, mas, apesar de sua importância, não há estudos de diversidade genética de populações virais. Foram determinadas as sequências nucleotídicas de parte do gene da proteína do capsídeo (CP) de seis isolados brasileiros de CPSMV. Os fragmentos genômicos (521 nucleotídeos) foram amplificados por RT-PCR, clonados e suas sequências foram comparadas entre si e com as sequências de outros comovírus. As comparações de sequências indicaram um elevado grau de conservação para o gene CP, com identidade de 92-100% (nucleotídeos) e 97-100% (aminoácidos) entre os isolados. Não houve correlação entre origem geográfica e identidade de sequência ou filogenia entre os isolados. Palavras-chave: Comovirus, Vigna unguiculata, CPSMV, diversidade de sequência.

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Diversity among isolates of squash mosaic virus.

Cited by 26 publications

References 19 publications

The C-terminal region of the movement protein of Cowpea mosaic virus is involved in binding to the large but not to the small coat protein

The C-terminal region of the movement protein of Cowpea mosaic virus is involved in binding to the large but not to the small coat protein

Structural Fingerprinting: Subgrouping of Comoviruses by Structural Studies of Red Clover Mottle Virus to 2.4-Å Resolution and Comparisons with Other Comoviruses

Sequence variability in the coat protein gene of Cowpea severe mosaic virus isolates from northeastern Brazil

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