The comovirus Cowpea severe mosaic virus (CPSMV) causes a disease in cowpea (Vigna unguiculata) but, despite its importance, there are no studies on the genetic diversity of viral populations. We have determined the nucleotide sequences of part of the coat protein (CP) gene of six Brazilian isolates of CPSMV. Genomic fragments (521 nucleotides) were RT-PCR amplified, cloned, and their sequences were compared with each other and with other comoviruses. Sequence comparisons indicated a high degree of conservation for the CP gene, with 92-100% nucleotide and 97-100% amino acid sequence identity among the isolates. There was no correlation between geographical origin and sequence identity or phylogeny among the isolates. Key words: Comovirus, Vigna unguiculata, CPSMV, sequence diversity. RESUMO Variabilidade de sequências do gene da proteína do capsídeo de isolados do Cowpea severe mosaic virus do Nordeste do BrasilO comovírus Cowpea severe mosaic virus (CPSMV) causa a doença em feijão-caupi (Vigna unguiculata) conhecida como mosaico severo, mas, apesar de sua importância, não há estudos de diversidade genética de populações virais. Foram determinadas as sequências nucleotídicas de parte do gene da proteína do capsídeo (CP) de seis isolados brasileiros de CPSMV. Os fragmentos genômicos (521 nucleotídeos) foram amplificados por RT-PCR, clonados e suas sequências foram comparadas entre si e com as sequências de outros comovírus. As comparações de sequências indicaram um elevado grau de conservação para o gene CP, com identidade de 92-100% (nucleotídeos) e 97-100% (aminoácidos) entre os isolados. Não houve correlação entre origem geográfica e identidade de sequência ou filogenia entre os isolados. Palavras-chave: Comovirus, Vigna unguiculata, CPSMV, diversidade de sequência.
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