Diversidade genética estimada com marcadores entre sequências simples repetidas em cultivos comerciais de Cupuaçuzeiro

Silva, Bruna Mezzalira da; Rossi, Ana Aparecida Bandini; Dardengo, Juliana de Freitas Encinas; Araujo, Vitor Arreguy Amado Correa de; Rossi, Fernanda S.; Oliveira, Luiz Orlando de; Clarindo, Wellington Ronildo

doi:10.1590/0103-8478cr20141634

Cited by 16 publications

(16 citation statements)

References 13 publications

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“…The Shannon index (I) was considered an intermediate value; according to Giustina et al (2014), Shannon indices (I) vary from 0 to 1, wherein 0 means null gene diversity and 1 is the maximum gene diversity. Silva et al (2016) assessed the genetic diversity estimated through microsatellite markers in commercial Cupuaçu crops and observed lower Nei diversity values (0.11) and Shannon index (I) (0.17) in comparison to values recorded in the current study. Nei genetic diversity index (H) values ranged from 0.20 to 0.24.…”

Section: Discussioncontrasting

confidence: 48%

Genetic structure of cassava populations (Manihot esculenta Crantz) from Angola assessed through (ISSR) markers

Afonso¹,

Moreira²,

Lêdo³

et al. 2019

Afr. J. Biotechnol.

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Eighteen ISSR primers (between Simple Repeated Sequences) were used to estimate the genetic diversity of Angolan cassava populations in order to identify genetic variabilities and their potential to be used in the diversification of cassava crops in Angola. In order to do so, three populations from three Angolan provinces were analyzed (Cuanza Norte, Uige and Malanje), which totaled 40 genotypes. In total, 116 bands were amplified with 93.24% polymorphism and 6.4 markers per ISSR primer, on average. Nei's gene diversity (H) and Shannon indices (I) applied to the analyzed accessions presented values ranging from H = 0.20 to 0.24 and I = 0.29 to 0.50 which are intermediate values. The total gene diversity (HT) was 0.3113, and it showed high heterozygosity in Manihot populations; this outcome indicated that these populations presented gene variability. ANOVA showed 10.41% total variance between populations and 89.59% within them. Molecular featuring revealed gene diversity in each of the assessed populations; there was gene structuring between populations.

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Section: Discussioncontrasting

confidence: 48%

Genetic structure of cassava populations (Manihot esculenta Crantz) from Angola assessed through (ISSR) markers

Afonso¹,

Moreira²,

Lêdo³

et al. 2019

Afr. J. Biotechnol.

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“…Em estudos semelhantes sobre as espécies, Giustina et al (2014) encontraram valores próximos aos relatados neste estudo para T. speciosum (H = 0,1672 e I = 0,2549) e Rivas et al (2013) encontraram H = 0,2612 e I = 0,3889 em populações naturais de T. subincanum. Silva et al (2016), em um estudo com populações cultivadas de Theobroma grandiflorum encontrou valores próximos de H e I (0,15 e 0,24 respectivamente). Considerando que estes índices variam de 0 a 1, onde 0 é considerado diversidade gênica nula e 1 diversidade gênica máxima , esses dados sugerem que a diversidade genética varia de moderada a baixa dentro da população de cada espécie estudada.…”

Section: Discussionunclassified

Diversidade genética de duas espécies de Theobroma L. em um fragmento florestal no Portal da Amazônia, MT, Brasil

Rossi

Silva

Arenas-de-Souza

et al. 2017

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Recebido em março/2017; Aceito em agosto/2017. RESUMO:O estudo objetivou analisar a diversidade genética de Theobroma speciosum e Theobroma subincanum em um fragmento florestal no município de Alta Floresta, Mato Grosso, Brasil. Foram amostrados aleatoriamente 21 indivíduos T. speciosum e 20 de T. subincanum. Sete primers ISSR foram utilizados e produziram 35 fragmentos dos quais 42,8% apresentaram polimorfismo para T. speciosum e 57,1% para T. subincanum. A diversidade genética de Nei e o Índice de Shannon foram de 0,133 e 0,201 para T. speciosum e 0,244 e 0,351 para T. subincanum respectivamente. O dendrograma UPGMA apresentou quatro grupos para T. speciosum e três grupos para T. subincanum. Os marcadores ISSR foram capazes de acessar a diversidade das duas espécies em estudo gerando informações para programas de conservação e melhoramento genético e demonstraram que a fragmentação vegetal ocasionou um efeito maior sobre a diversidade genética da espécie T. speciosum nas populações estudadas. Os níveis de diversidade encontrados no estudo indicam existência de variabilidade genética e alertam para a importância da conservação in situ destas populações como fonte de genes para o melhoramento genético do gênero Theobroma. Palavras-chave: Cacauí. Cupuí, fragmentação florestal, ISSR.Genetic diversity of two species of Theobroma L. in a forest fragment in Amazon Portal, MT, Brazil ABSTRACT:This study aimed to analyze the genetic diversity of Theobroma speciosum and Theobroma subincanum in forest fragment in the municipality of Alta Floresta, Mato Grosso, Brazil. We randomly sampled twenty-one T. speciosum individuals and twenty T. subincanum individuals. The seven ISSR primers used produced 35 fragments, from which 42,8% were polymorphic for T. speciosum and 57,1 % were polymorphic for T. subincanum. The Nei's genetic diversity and Shannon index respectively were 0,133 and 0,201 for T. speciosum and 0,244 and 0,351 for T. subincanum. The UPGMA dendrogram showed four clusters for T. speciosum and three clusters for T. subincanum. The ISSR markers were able to access the genetic diversity of the two species studied, thus they provided information for conservation and breeding programs, also demonstrated that forest fragmentation caused a greater effect on genetic diversity of the T. speciosum species in the populations studied. The levels of diversity found in the study indicate that there is genetic variability and alert to the importance of in situ conservation of these populations as a source of genes for the genetic improvement of the genus Theobroma.

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“…ISSR (inter-simple sequence repeat) molecular markers is an excellent tool for the genetic diversity analysis and characterization of accessions and cultivars of several species due to the high degree of polymorphism, reproducibility and low cost (Salazar-Laureles et al, 2015;Brito et al, 2016;Silva et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Desmanthus sp accessions using ISSR markers and morphological traits

Costa

Fracetto

et al. 2017

Genet. Mol. Res.

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ABSTRACT. Desmanthus is a genus of forage legumes with high nutritional value, productive potential, and ability to obtain nitrogen in association with diazotrophic bacteria. The use of accurate techniques for genotype identification and characterization is essential for breeding programs. Morphological markers are widely used to know the genetic diversity and the molecular markers are fundamental in these studies. We investigated the genetic diversity among Desmanthus sp genotypes in Pernambuco (Brazilian Northeast State), using morphological traits and ISSR markers. Morphological and molecular characterizations were performed in 18 and 26 accessions, respectively, in plants belonging to the germplasm bank of forage legumes of Universidade Federal Rural de Pernambuco (UFRPE), Academic Unit of Serra Talhada, PE, Brazil. Eight ISSR primers were selected, and 95 loci were generated, with polymorphism of 95.79%. The allele number observed was 1.958, where the effective number was 1.359, and the Nei diversity genetic 2 J.C. Costa et al.Genetics and Molecular Research 16 (2): gmr16029667 index was 0.226. About the morphological markers, seed number was the variable that most contributed to the genetic divergence. A large amount of genetic diversity was observed among Desmanthus species, occurring spontaneously in Pernambuco, Brazil. Thus, the variability found in morphological and ISSR markers is importance for the improvement of Desmanthus spp. Our findings showed that 17L, 27L, 25F, 22F, 19S, 13Au, and 28G accessions could be used in breeding programs to explore the maximum genetic divergence.

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Diversidade genética estimada com marcadores entre sequências simples repetidas em cultivos comerciais de Cupuaçuzeiro

Abstract: RESUMO Quinze primers ISSR (entre sequências simples repetidas) foram utilizados para avaliar a diversidade genética entre e dentro de pomares comerciais de

Cited by 16 publications

References 13 publications

Genetic structure of cassava populations (Manihot esculenta Crantz) from Angola assessed through (ISSR) markers

Genetic structure of cassava populations (Manihot esculenta Crantz) from Angola assessed through (ISSR) markers

Diversidade genética de duas espécies de Theobroma L. em um fragmento florestal no Portal da Amazônia, MT, Brasil

Genetic diversity of Desmanthus sp accessions using ISSR markers and morphological traits

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