Diversidade genética de Copernicia prunifera com o uso de marcadores moleculares ISSR

Abstract

RESUMOO objetivo desse estudo foi selecionar primers ISSR (Inter Simple Sequence Repeat) para estudos da estrutura genética, assim como quantificar a variabilidade genética de uma população natural da C. prunifera. (UBC 841). O número ótimo de locos para se estimar confiavelmente a diversidade genética para esse trabalho foi de 76 locos. Foi encontrada alta diversidade genética, com o número de alelos observados (na = 2,00), alelos efetivos (ne = 1,46), índice de diversidade de Nei (He = 0,28) e índice de Shannon (Ho = 0,44). O valor da coancestria se manteve dentro do intervalo de confiança (P > 0,05), indicando ausência de estrutura genética espacial. Os marcadores ISSR se mostraram eficientes na caracterização genotípica dos indivíduos de carnaúba, servindo como subsídio para planos de manejo e conservação da espécie.Palavras-chave: Arecaceae, carnaúba, nordeste do Brasil, variação genética Genetic diversity of Copernicia prunifera using ISSR molecular markers ABSTRACTThe aim of this study was to select primers ISSR (Inter Simple Sequence Repeat) for studies of the genetic structure, and to quantify the genetic variability in a natural population of C. prunifera. Thirty seven individuals were sampled in the municipality Macaíba, Rio Grande do Norte, Northeast Brazil. Seventeen ISSR primers were tested, of which 12 amplified DNA and of these, seven were selected to characterize the structure and genetic diversity of the population. The primers that had the highest percentage of polymorphic loci were UBC 841 (16.36%), UBC 842 (15.45%), UBC 857 (12.73%), UBC 859 (10.90%), UBC 840 (10.90%), UBC 813 (10%) and UBC 827 (8.18%), totaling 93 of the 110 loci generated. The polymorphic information content (PIC) of each selected primer ranged from 0.057 (UBC 859) and 0.444 (UBC 841). The optimal number of loci to reliably estimate the genetic diversity for this work was 76 loci, ie, when the correlation reached 0.998 and the stress was less than 0.05. High genetic diversity was found, with the number of observed alleles (na = 2.00), effective alleles (ne = 1.46), Nei's diversity index (He = 0.28) and Shannon index (Ho = 0.44). The value of the coancestry remained within the confidence interval (P > 0.05), indicating no spatial genetic structure, regardless of the distance between plants. ISSR markers were efficient in genetic characterization of individuals' carnauba serving with allowance for management and conservation of the species.

Genetic diversity of Desmanthus sp accessions using ISSR markers and morphological traits

“…The highest values were found when the primer UBC812 was used with values of 0.456 for H and 0.303 for I. Our values were similar to the ones reported in the literature for dominant markers (Vieira et al, 2015).…”

“…According to Kruskal (1964), when the stress value is equal to or less than 0.05 the estimates are accurate. For Copernicia prunifera, 76 bands were sufficient to estimate the genetic variability (Vieira et al, 2015). The primer UBC1 presented the lowest polymorphism (90%), whereas the primers UBC02, UBC808, UBC810, and UBC834 showed 100% polymorphism ( Table 2).…”

“…Morphological markers are widely used; however, they may present low efficiency in the assessment of available variability (Silva et al, 2013). On the other hand, molecular markers are highly accurate because they detect differences in DNA level and exhibit sufficient polymorphism to discriminate genotypes (Vieira et al, 2015).…”

Genetic diversity in natural populations of Stylosanthes scabra using ISSR markers

“…Four types of markers are used during the genetic diversity characterization: morphological, biochemical, molecular, and cytological. The molecular types present the particularity of use under analysis at any stage of plant development (Martuscello et al, 2015, Vieira et al, 2015. Among molecular markers, ISSRs (Inter Simple Sequence Repeat) stand out, because they do not require prior information of the DNA sequence and present laboratory procedures with good transferability (Dias et al, 2015).…”

Research Article Anthropization as a determinant factor in the genetic structure of Copernicia prunifera (Arecaceae).

“…Despite there being few studies about seed and pollen dispersion of Copernicia prunifera, its main pollinators have been identified as the "marimbondo caboclo" wasp (Polistes canadensis Linnaeus) and the "irapuá" bee (Trigona spinipes Fabricius), while bats and the palm tanager "sanhaçu-do-coqueiro" (Tangara palmarum) are the main seed dispersers (Silva RAR, Fajardo CG, and Vieira FA, unpublished data;Vieira et al, 2015). However, the efficiency of these dispersal vectors for C. prunifera is not yet well established, and natural populations of the species continue to be subjected to extensive anthropogenic pressures.…”