Diverse Triterpenoids from Mastic Produced by Pistacia lentiscus and Their Anti‐Inflammatory Activities

Abstract

Eight undescribed triterpenoids (1 -8), including one apotirucallane-type triterpenoid (1), six tirucallane-type triterpenoids (2-7), and one oleanane-type triterpenoid (8), along with ten known compounds (9 -18) were isolated from the resins of Pistacia lentiscus. Their structures were elucidated by integrating NMR spectroscopic analyses and ESI-HR-MS. Compounds 5, 11-17 exhibited moderate inhibitory abilities against NO production in LPS-induced RAW264.7 cells, with IC 50 values in the range of 18.26 -50.37 μM, compared to that of the positive control dexamethasone (IC 50 = 20.24 μM).

Triterpenoid and Steroid Content of Lipophilic Extracts of Selected Medicinal Plants of the Mediterranean Region

“…It could be expected according to the reports on the triterpenoid content of this plant, concerning mainly the resin called mastic. The composition of triterpenoids in P. lentiscus mastic have been reported as particularly complex, comprising tetra- and pentacyclic triterpenoids as tirucallol, dammaradienone, β-amyrin, lupeol, oleanolic aldehyde, 28-norolean-12-en-3-one, as well as masticadienonic, isomasticadienonic, morolic, oleanonic, ursonic acids, and many other compounds [ 50 , 51 , 52 ]; moreover, the recent reports have been expanding this list [ 53 ]. P. lentiscus is also rich in other terpenoids present in essential oils, such as β-pinene, camphene, myrcene, limonene, β-caryophyllene and linalool [ 50 ].…”

Sesquiterpenes fromRhododendron nivaleand Their Anti‐Inflammatory Activity

“…The anti-inflammatory activity was screened by measuring NO production in RAW 264.7 cells according to methods published. [19] RAW264.7 cells were cultured in 96-well plates at a density of 5 × 10 4 cells/well for 24 h. The cells were stimulated with LPS (1 μg/mL) and then cultured with different concentrations of samples and positive control for 24 h. Then, 50 μL of the supernatant was removed and Griess reagent (50 μL of Reagent A and 50 μL of Reagent B) was added to each well. Finally, the absorbance value was measured at 540 nm.…”