Diverse microbial community from the coalbeds of the Ordos Basin, China

Tang, Yue‐Qin; Ji, Peng; Lai, Guo-Li; Chi, Chang-Qiao; Liu, Ze-Shen; Wu, Xiao‐Lei

doi:10.1016/j.coal.2011.09.009

Cited by 56 publications

(46 citation statements)

References 77 publications

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“…Microbial communities recovered from across the Alberta Basin displayed patterns of endemism, wherein different microbial consortia were unique to defined geographical regions that exhibited variation in coal rank and physicochemical condition correlating with depth. This result was consistent with reports from other coalbed ecosystems that also observed significant diversity between microbial communities spanning different coal formations (8,9,11,60,61). Indeed, differences in coal rank are expected to influence CBM production as low-maturity coals (e.g., subbituminous coals) are less recalcitrant and more biodegradable than higher-maturity coals (e.g., volatile bituminous coals) due to changes in carbon and oxygen content (8).…”

Section: Discussionsupporting

confidence: 81%

“…In summary, our results show that mi-crobial community structure displayed patterns of endemism and habitat selection across coalbed formations within the Alberta Basin, consistent with observations for other geographical locations (8,9,11,60,61). While some phylum-level taxonomic patterns were observed, the relative abundance of specific taxonomic groups was localized to discrete wells likely shaped by habitatspecific environmental conditions, such as coal rank and depthdependent physicochemical conditions.…”

Section: Discussionsupporting

confidence: 71%

“…Microbial diversity surveys using small-subunit rRNA (SSU; or 16S rRNA) gene sequencing have been conducted across geographically distinct coalbed ecosystems (7)(8)(9)(10)(11) and enrichment cultures (6,12). Taxonomic groups, including Firmicutes, Spirochetes, Bacteroidetes, Proteobacteria (Alpha-, Beta-, Gamma-, and Deltaproteobacteria) and Euryarchaeota, with the potential to mediate syntrophic interactions driving CBM production have been observed in many coalbed ecosystems.…”

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confidence: 99%

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Patterns of Endemism and Habitat Selection in Coalbed Microbial Communities

Lawson

Strachan

Williams³

et al. 2015

Appl Environ Microbiol

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dMicrobially produced methane, a versatile, cleaner-burning alternative energy resource to fossil fuels, is sourced from a variety of natural and engineered ecosystems, including marine sediments, anaerobic digesters, shales, and coalbeds. There is a prevailing interest in developing environmental biotechnologies to enhance methane production. Here, we use small-subunit rRNA gene sequencing and metagenomics to better describe the interplay between coalbed methane (CBM) well conditions and microbial communities in the Alberta Basin. Our results show that CBM microbial community structures display patterns of endemism and habitat selection across the Alberta Basin, consistent with observations from other geographical locations. While some phylum-level taxonomic patterns were observed, relative abundances of specific taxonomic groups were localized to discrete wells, likely shaped by local environmental conditions, such as coal rank and depth-dependent physicochemical conditions. To better resolve functional potential within the CBM milieu, a metagenome from a deep volatile-bituminous coal sample was generated. This sample was dominated by Rhodobacteraceae genotypes, resolving a near-complete population genome bin related to Celeribacter sp. that encoded metabolic pathways for the degradation of a wide range of aromatic compounds and the production of methanogenic substrates via acidogenic fermentation. Genomic comparisons between the Celeribacter sp. population genome and related organisms isolated from different environments reflected habitat-specific selection pressures that included nitrogen availability and the ability to utilize diverse carbon substrates. Taken together, our observations reveal that both endemism and metabolic specialization should be considered in the development of biostimulation strategies for nonproductive wells or for those with declining productivity.T he deposition of plant-derived organic matter over geological time has resulted in the formation of stratified hydrocarbon resource environments known as coalbeds. This prevalent energy resource has fueled human development for thousands of years with concomitant environmental impacts, including landscape alteration, waste production, and greenhouse gas emissions (1, 2). In contrast to solid fuel, cleaner-burning coalbed methane (CBM) has become an increasingly attractive global energy resource. While some fraction of CBM is produced under thermogenic conditions, recent studies indicate that microbial communities inhabiting coalbed ecosystems contribute substantially to methane production (3). This has sparked both scientific and biotechnological interest in coalbed microbial communities to enhance CBM production from wells with low methane content or declining productivity (4-6).Microbial diversity surveys using small-subunit rRNA (SSU; or 16S rRNA) gene sequencing have been conducted across geographically distinct coalbed ecosystems (7-11) and enrichment cultures (6, 12). Taxonomic groups, including Firmicutes, Spirochetes, Bacteroidete...

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Section: Discussionsupporting

confidence: 81%

Section: Discussionsupporting

confidence: 71%

mentioning

confidence: 99%

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Patterns of Endemism and Habitat Selection in Coalbed Microbial Communities

Lawson

Strachan

Williams³

et al. 2015

Appl Environ Microbiol

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“…While Methanosaeta, Methanosarcina and Methanobacterium have been found to be dominant in other coal associated microbial communities Tang et al, 2012), the dominance of Methanoregula has not yet been reported.…”

Section: Microbial Methanogenesis Processmentioning

confidence: 99%

“…The terminal step of coal hydrolysis by microorganisms is methanogenesis, in which the acetate, H2 and CO2, and the methanol or methylamine, are transformed into CH4 by acetoclastic, hydrogenotrophic and methylotrophic methanogens, respectively (Table 2) (Strąpoć et al, 2011). Bacterial species from the genus of Bacteroides, Geobacter, Clostridium, and Spirochaeta, are known geopolymer hydrolysers that have been detected in many biogenic CBM reservoirs worldwide, and are typically found associated with archaea belonging to the hydrogenotrophic methanogenic genera Methanobacterium, Methanocorpusculum and Methanolobus, and acetoclastic genera Methanosaeta and Methanosarcina (Doerfert et al, 2009;Ghosh et al, 2014;Midgley et al, 2010;Singh et al, 2012;Strąpoć et al, 2008;Tang et al, 2012). Despite a significant quantity of research on microbial methanogenesis in coal formation, the microbial community composition and its role in coal biodegradation are still not well understood.…”

Section: Microbial Methanogen Consortiamentioning

confidence: 99%

Biogenic methane in Indonesian coal : assessing the potential using culture enrichment, molecular phylogenetic and isotopic analysis

Susilawati¹

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Biogenic coal bed methane (CBM) has gained considerable interest because of its potential to be regenerated. The research reported in this thesis evaluated the potential to develop Indonesian coals as CH 4 bioreactors using culture enrichment, molecular phylogenetic and isotopic composition analysis methods. The results increase the current understanding on microbial methanogenesis in coal, with a specific focus on Indonesian coals.Indonesian coals range in rank from lignite to bituminous, with anthracites occurring in specific areas. The coals are low in ash yield and dominated by vitrinite group macerals, with abundant liptinite group macerals, which is thought to make them good targets for methanogenesis.Stable isotope analysis of gas from pilot production wells confirmed the hypothesis that Indonesian CBM is partly biogenic in origin. Culture enrichment studies of formation water from these CBM pilot wells also indicated the presence of active coal to CH 4 consortia that has the capability for degrading native South Sumatra Basin (SSB) coals and foreign Surat Basin coal from Australia. Among the Indonesian coal basins, the SSB currently has the greatest CBM potential and good accessibility for gas, water, and coal sampling that is required to assess the biogenic CH 4 potential of Indonesian coal. Therefore, SSB was targeted for the experiments conducted in this thesis. The investigation of the microbial methanogen community structure grown on different coal substrates showed temporal changes in community structure over time, and suggested some influence of coal substrate on the microbial community composition. The community structure exhibited greater similarity when grown on coal from the same seam but of different ranks (Mangus iii sub-bituminous (SB) Rv 0.5% and Mangus Anthracite (A) Rv 2.2%) than for coal of similar types (Mangus SB Rv 0.5% and Burung SB Rv 0.39%). The obligate acetoclastic Methanosaeta members favor Burung SB coal, while metabolically versatile Methanosarcina members favor the Mangus coals and the obligate hydrogenotrophic methanogens are significant in the control cultures without coal. Regardless of the community structure similarities across all coal cultures, more CH 4 was generated from the lower rank sub-bituminous coal cultures relative to the one high-rank semi anthracite coal. These results suggest a potential relationship between coal type and rank, microbial community composition and CH 4 production, which warrants further investigation.While CH 4 measurements and molecular phylogenetic analysis confirmed the production of biogenic CH 4 in the cultures, the gas sampled from the culture headspace had δ 13 C-CH 4 values ( 52.2‰ to 22.6‰) that mostly fell outside the range currently considered to indicate a biogenic origin. In this study, the apparent carbon fractionation factor (α c =1.02±0.006) and isotope effect (ε c = 20.1‰±15.3) were found to be more useful indicators of methanogenic pathways than the absolute δ 13 C-CH 4 values. Both values agreed with the calculat...

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“ Psychrodesulfovibrio ” gen. nov.

Galushko

Kuever

2020

Bergey's Manual of Systematics of Archaea and Bacteria

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Psy.chro.de.sul.fo.vi'bri.o. Gr. masc. adj. psychros, cold; N.L. masc. n. Desulfovibrio, a bacterial genus; N.L. masc. n. Psychrodesulfovibrio , a Desulfovibrio living in the cold. Desulfobacterota / Desulfovibrionia / Desulfovibrionales / Desulfovibrionaceae / Psychrodesulfovibrio gen. nov. Cells are vibrio‐shaped, 0.3–0.8 × 0.5–2.0 μm. Occur singly or in pairs. Spore formation is not observed. Stain Gram‐negative. Cells are motile by a single polar flagellum. Strictly anaerobic, having respiratory and fermentative types of metabolism. Chemoorganoheterotrophic. Lactate, pyruvate, succinate, malate, ethanol, and butanol serve as electron donors and carbon sources and are incompletely oxidized to acetate. Formate can serve as an electron donor in the presence of acetate as an organic carbon source. H 2 plus CO 2 is not used. Sulfate serves as a terminal electron acceptor and is reduced to H 2 S. Desulfoviridin is present. Fermentative growth on pyruvate and malate. Mesophilic, the optimum temperature for growth is 28–30°C. Slightly alkaliphilic and aerotolerant. Media containing a reductant and p ‐aminobenzoate are required for growth. Occur in freshwater, brackish water, and habitats with marine NaCl concentrations such as oil fields. DNA G + C content (mol%) : 49.3 (LC). Type species : “ Psychrodesulfovibrio psychrotolerans ” comb. nov. (basonym: Desulfovibrio psychrotolerans Sasi Jyothsna et al., 2008 VP ).

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Diverse microbial community from the coalbeds of the Ordos Basin, China

Cited by 56 publications

References 77 publications

Patterns of Endemism and Habitat Selection in Coalbed Microbial Communities

Patterns of Endemism and Habitat Selection in Coalbed Microbial Communities

Biogenic methane in Indonesian coal : assessing the potential using culture enrichment, molecular phylogenetic and isotopic analysis

“ Psychrodesulfovibrio ” gen. nov.

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